Mouse Anti-FBXL7 Recombinant Antibody (CBXF-0389) (CBMAB-F0352-CQ)

Name: Mouse Anti-FBXL7 Recombinant Antibody (CBXF-0389)
SKU: CBMAB-F0352-CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-FBXL7 Recombinant Antibody (CBXF-0389) (CBMAB-F0352-CQ) in WB

Western blot analysis showing the elevated FBXL7 protein expression in A549 and H1650 cells transduced with oe-FBXL7. ...View More

Zhou, J., Lin, Y., Kang, X., Liu, Z., Zou, J., & Xu, F. (2023). Hypoxia-mediated promotion of glucose metabolism in non-small cell lung cancer correlates with activation of the EZH2/FBXL7/PFKFB4 axis. Cell Death & Disease, 14(5), 326.

Mouse Anti-FBXL7 Recombinant Antibody (CBXF-0389) (CBMAB-F0352-CQ) in IHC

Immunohistochemical staining analysis of Ki-67, EZH2, PFKFB4, and FBXL7 proteins in the tumor tissues of nude mice subcutaneously inoculated with cells transduced with sh-EZH2 alone or combined with oe-PFKFB4. ...View More

Mouse Anti-FBXL7 Recombinant Antibody (CBXF-0389) (CBMAB-F0352-CQ) in IHC

Representative images of immunohistochemical staining of FBXL7, AURKA and BIR5 in esophageal (ESCC), larynx (LSCC) and oropharynx squamous cell carcinoma (OPSCC). ...View More

Camuzi, D., Buexm, L. A., Lourenço, S. D. Q. C., Grazziotin, R., Guaraldi, S., Valverde, P., ... & Soares-Lima, S. C. (2022). FBXL7 body hypomethylation is frequent in tumors from the digestive and respiratory tracts and is associated with risk-factor exposure. International Journal of Molecular Sciences, 23(14), 7801.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXF-0389

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 367-405 within an internal region of human FBL7.

Specificity

Human, Mouse, Rat, Horse, Dog, Pig, Chicken

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 392-490

More Infomation

Target

Full Name

F-box and leucine-rich repeat protein 7

Introduction

This gene encodes a member of the F-box protein family which is characterized by a 42-48 amino acid motif, the F-box, which binds to the S-phase kinase-associated protein 1 (Skp1) protein. The F-box proteins constitute one of the four subunits of E3 ubiquitin protein ligases called SCFs (SKP1-Cul1-F-box), which play a role in phosphorylation-dependent ubiquitination of proteins. The F-box proteins are divided into 3 subfamilies based on the other domain in the protein: F-box proteins that also have a WD-40 domain (Fbws subfamily), F-box proteins that also have leucine-rich repeats (Fbls subfamily) and F-box proteins that contain other motifs or lack known protein-interaction domains (Fbxs subfamily). The protein encoded by this gene belongs to the Fbls subfamily. Alternative splicing results in multiple transcript variants of this gene.

Entrez Gene ID

23194

UniProt ID

Q9UJT9

Alternative Names

F-Box And Leucine Rich Repeat Protein 7; FBL6; FBL7; F-Box And Leucine-Rich Repeat Protein 7; F-Box/LRR-Repeat Protein 7; F-Box Protein FBL6/FBL7; F-Box Protein Fbl7; KIAA0840;

Research Area

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex (PubMed:25778398).

During mitosis, it mediates the ubiquitination and subsequent proteasomal degradation of AURKA, causing mitotic arrest (By similarity).

It also regulates mitochondrial function by mediating the ubiquitination and proteasomal degradation of the apoptosis inhibitor BIRC5 (PubMed:25778398, PubMed:28218735).

Biological Process

Cell division Source: UniProtKB-KW
G2/M transition of mitotic cell cycle Source: UniProtKB
Mitotic cell cycle Source: UniProtKB
Protein polyubiquitination Source: UniProtKB
Protein ubiquitination Source: UniProtKB
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Ubiquitin-dependent protein catabolic process Source: UniProtKB

Cellular Location

Centrosome. Localizes to the centrosome during spindle formation.

Wang, Y., Shen, X., Gong, L., Zhao, Y., & Xiong, X. (2022). Functional characterization of FBXL7 as a novel player in human cancers. Cell Death Discovery, 8(1), 1-8.

Ni, J., Shen, L., Xu, L., Jin, Q., & Wang, G. (2021). 7-Ethoxyrosmanol alleviates hyperglycemia-induced vascular endothelial dysfunction by regulating FBXL7 expression. Journal of Bioenergetics and Biomembranes, 53(5), 553-560.

Moro, L., Simoneschi, D., Kurz, E., Arbini, A. A., Jang, S., Guaragnella, N., ... & Pagano, M. (2020). Epigenetic silencing of the ubiquitin ligase subunit FBXL7 impairs c-SRC degradation and promotes epithelial-to-mesenchymal transition and metastasis. Nature cell biology, 22(9), 1130-1142.

Boone, P. M., Paterson, S., Mohajeri, K., Zhu, W., Genetti, C. A., Tai, D. J., ... & Rodan, L. H. (2020). Biallelic mutation of FBXL7 suggests a novel form of Hennekam syndrome. American Journal of Medical Genetics Part A, 182(1), 189-194.

Kong, S., Fang, Y., Wang, B., Cao, Y., He, R., & Zhao, Z. (2020). miR‐152‐5p suppresses glioma progression and tumorigenesis and potentiates temozolomide sensitivity by targeting FBXL7. Journal of Cellular and Molecular Medicine, 24(8), 4569-4579.

Moro, L., & Pagano, M. (2020). Epigenetic suppression of FBXL7 promotes metastasis. Molecular & Cellular Oncology, 7(6), 1833698.

Chiu, H. W., Chang, J. S., Lin, H. Y., Lee, H. H., Kuei, C. H., Lin, C. H., ... & Lin, Y. F. (2018). FBXL7 upregulation predicts a poor prognosis and associates with a possible mechanism for paclitaxel resistance in ovarian cancer. Journal of clinical medicine, 7(10), 330.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-FBXL7 Recombinant Antibody (CBXF-0388) (CAT#: V2LY-0925-LY407)

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For research use only. Not intended for any clinical use.

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