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Mouse Anti-FGF1 Monoclonal Antibody (Y37C) (CBMAB-1258-YC)

Provided herein is a mouse monoclonal antibody against Human FGF1. The antibody, clone Y37C, can be used for immunoassay techniques, such as ELISA, ICC, IHC-Fr, IHC-P and WB.
See all FGF1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
Y37C
Antibody Isotype
IgG
Application
ELISA, ICC, IHC-Fr, IHC-P, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Fibroblast Growth Factor 1
Introduction
FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF1 is a member of the fibroblast growth factor (FGF) family, which functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor.
Entrez Gene ID
2246
UniProt ID
P05230
Alternative Names
AFGF; ECGF; FGFA; ECGFA; ECGFB; FGF-1; HBGF1; HBGF-1; GLIO703; ECGF-beta; FGF-alpha
Research Area
Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed:18441324, PubMed:20422052).

Can induce angiogenesis (PubMed:23469107).
Biological Process
Activation of MAPK activity Source: UniProtKB
Activation of protein kinase B activity Source: UniProtKB
Anatomical structure morphogenesis Source: ProtInc
Angiogenesis Source: UniProtKB-KW
Animal organ morphogenesis Source: GO_Central
Branch elongation involved in ureteric bud branching Source: UniProtKB
Cell differentiation Source: GO_Central
Cellular response to heat Source: UniProtKB
Fibroblast growth factor receptor signaling pathway Source: UniProtKB
Lung development Source: GO_Central
Mesonephric epithelium development Source: UniProtKB
Multicellular organism development Source: ProtInc
Organ induction Source: Ensembl
Positive regulation of angiogenesis Source: UniProtKB
Positive regulation of cell division Source: UniProtKB
Positive regulation of cell migration Source: UniProtKB
Positive regulation of cell population proliferation Source: UniProtKB
Positive regulation of cholesterol biosynthetic process Source: BHF-UCL
Positive regulation of endothelial cell migration Source: UniProtKB
Positive regulation of epithelial cell proliferation Source: GO_Central
Positive regulation of gene expression Source: GO_Central
Positive regulation of hepatocyte proliferation Source: Ensembl
Positive regulation of intracellular signal transduction Source: BHF-UCL
Positive regulation of MAP kinase activity Source: UniProtKB
Positive regulation of protein phosphorylation Source: GO_Central
Positive regulation of sprouting angiogenesis Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Regulation of cell migration Source: GO_Central
Regulation of endothelial cell chemotaxis to fibroblast growth factor Source: UniProtKB
Regulation of endothelial tube morphogenesis Source: UniProtKB
Signal transduction Source: ProtInc
Wound healing Source: UniProtKB
Cellular Location
Secreted; Nucleus; Cytoplasm; Cytosol; Cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu2+ ions and S100A13. Secreted in a complex with SYT1 (By similarity). Binding of exogenous FGF1 to FGFR facilitates endocytosis followed by translocation of FGF1 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as LRRC59.
PTM
In the nucleus, phosphorylated by PKC/PRKCD.

Gasser, E., Sancar, G., Downes, M., & Evans, R. M. (2022). Metabolic Messengers: fibroblast growth factor 1. Nature Metabolism, 1-9.

Sluzalska, K. D., Slawski, J., Sochacka, M., Lampart, A., Otlewski, J., & Zakrzewska, M. (2021). Intracellular partners of fibroblast growth factors 1 and 2-implications for functions. Cytokine & growth factor reviews, 57, 93-111.

Weant, J., Eveleth, D. D., Subramaniam, A., Jenkins-Eveleth, J., Blaber, M., Li, L., ... & Bradshaw, R. A. (2021). Regenerative responses of rabbit corneal endothelial cells to stimulation by fibroblast growth factor 1 (FGF1) derivatives, TTHX1001 and TTHX1114. Growth Factors, 1-14.

Lin, Q., Huang, Z., Cai, G., Fan, X., Yan, X., Liu, Z., ... & Tan, Y. (2021). Activating adenosine monophosphate–activated protein kinase mediates fibroblast growth factor 1 protection from nonalcoholic fatty liver disease in mice. Hepatology, 73(6), 2206-2222.

Xiao, M., Tang, Y., Lu, G., Guo, Y., Zhang, J., & Gu, J. (2021). Regulatory role of endogenous and exogenous fibroblast growth factor 1 in the cardiovascular system and related diseases. Pharmacological Research, 169, 105596.

Bentsen, M. A., Rausch, D. M., Mirzadeh, Z., Muta, K., Scarlett, J. M., Brown, J. M., ... & Pers, T. H. (2020). Transcriptomic analysis links diverse hypothalamic cell types to fibroblast growth factor 1-induced sustained diabetes remission. Nature communications, 11(1), 1-16.

Xu, Z., Wu, Y., Wang, F., Li, X., Wang, P., Li, Y., ... & Liu, Y. (2020). Fibroblast growth factor 1 ameliorates diabetes-induced liver injury by reducing cellular stress and restoring autophagy. Frontiers in Pharmacology, 11, 52.

Xu, Z., Zhou, X., & Chen, G. (2019). Expression and mechanism of interleukin 1 (IL-1), interleukin 2 (IL-2), interleukin 8 (IL-8), BMP, fibroblast growth factor 1 (FGF1), and insulin-like growth factor (IGF-1) in lumbar disc herniation. Medical science monitor: international medical journal of experimental and clinical research, 25, 984.

Fan, L., Ding, L., Lan, J., Niu, J., He, Y., & Song, L. (2019). Fibroblast growth factor-1 improves insulin resistance via repression of JNK-mediated inflammation. Frontiers in pharmacology, 10, 1478.

Wu, Y., Li, Y., Jiang, T., Yuan, Y., Li, R., Xu, Z., ... & Xiao, J. (2018). Reduction of cellular stress is essential for Fibroblast growth factor 1 treatment for diabetic nephropathy. Journal of Cellular and Molecular Medicine, 22(12), 6294-6303.

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For research use only. Not intended for any clinical use.

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