Mouse Anti-FHL3 Recombinant Antibody (2C10) (CBMAB-A2984-LY)

The product is antibody recognizes FHL3. The antibody 2C10 immunoassay techniques such as: WB, ELISA.
See all FHL3 antibodies

Summary

Host Animal

Mouse

Specificity

Human

Clone

2C10

Antibody Isotype

IgG2a, κ

Application

WB, ELISA

Basic Information

Immunogen

FHL3 (NP_004459.2, 3 a.a. ~ 102 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

four and a half LIM domains 3

Introduction

LIM proteins are defined by the possession of a highly conserved double zinc finger motif called the LIM domain.[supplied by OMIM

Entrez Gene ID

2275

UniProt ID

Q13643

Alternative Names

MGC19547; MGC23614; MGC8696; SLIM2

Function

Recruited by SOX15 to FOXK1 promoters where it acts as a transcriptional coactivator of FOXK1.

Biological Process

Actin cytoskeleton organization Source: GO_Central
Muscle organ development Source: ProtInc

Cellular Location

Cytoplasm; Nucleus

References

Alkobtawi, M., Pla, P., & Monsoro-Burq, A. H. (2021). BMP signaling is enhanced intracellularly by FHL3 controlling WNT-dependent spatiotemporal emergence of the neural crest. Cell Reports, 35(12), 109289.

Cao, G., Li, P., He, X., Jin, M., Li, M., Chen, S., ... & Chen, B. (2021). FHL3 Contributes to EMT and Chemotherapy Resistance Through Up-Regulation of Slug and Activation of TGF β/Smad-Independent Pathways in Gastric Cancer. Frontiers in oncology, 11, 649029.

Li, P., Cao, G., Zhang, Y., Shi, J., Cai, K., Zhen, L., ... & Wu, Y. (2020). FHL3 promotes pancreatic cancer invasion and metastasis through preventing the ubiquitination degradation of EMT associated transcription factors. Aging (Albany NY), 12(1), 53.

Nishikawa, M., Sato, K., Nakano, S., Yamakawa, H., Nagase, T., & Ueda, H. (2019). Specific activation of PLEKHG2-induced serum response element-dependent gene transcription by four-and-a-half LIM domains (FHL) 1, but not FHL2 or FHL3. Small GTPases, 10(5), 361-366.

Han, S., Cui, C., Wang, Y., He, H., Shen, X., Chen, Y., ... & Yin, H. (2019). FHL3 negatively regulates the differentiation of skeletal muscle satellite cells in chicken. 3 Biotech, 9(6), 1-8.

Han, W., Hu, P., Wu, F., Wang, S., Hu, Y., Li, S., ... & Peng, X. (2019). FHL3 links cell growth and self-renewal by modulating SOX4 in glioma. Cell Death & Differentiation, 26(5), 796-811.

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Mouse Anti-FHL3 Recombinant Antibody (2C10)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Rabbit Anti-FHL3 Recombinant Antibody (CBXF-1119)

Mouse Anti-FHL3 Recombinant Antibody (CBXF-3026)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote

Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: