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Mouse Anti-FICD Recombinant Antibody (CBXF-3030) (CBMAB-F3736-CQ)

This product is a mouse antibody that recognizes FICD. The antibody CBXF-3030 can be used for immunoassay techniques such as: ELISA, WB.
See all FICD antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBXF-3030
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
Full-length Recombinant protein corresponding to aa1-450 from HYPE (AAH013420) with GST tag
Specificity
Human
Antibody Isotype
IgG2a, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
FIC Domain Containing
Introduction
FICD (FIC Domain Containing) is a Protein Coding gene. Diseases associated with FICD include Depersonalization Disorder and Extragonadal Germ Cell Cancer. Gene Ontology (GO) annotations related to this gene include protein adenylyltransferase activity.
Entrez Gene ID
11153
UniProt ID
Q9BVA6
Alternative Names
FIC Domain Containing; Huntingtin-Interacting Protein 13; Huntingtin-Interacting Protein E; FIC Domain-Containing Protein; Huntingtin Yeast Partner E; AMPylator FICD; HIP-13; HIP13; HYPE;
Function
Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context (By similarity).

The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place (By similarity).

Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP (PubMed:25601083).

In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it (By similarity).

In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity (By similarity).

Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates (PubMed:19362538, PubMed:25601083).
Biological Process
Negative regulation of GTPase activity Source: UniProtKB
Protein adenylylation Source: UniProtKB
Protein deadenylylation Source: UniProtKB
Regulation of IRE1-mediated unfolded protein response Source: UniProtKB
Response to endoplasmic reticulum stress Source: UniProtKB
Response to unfolded protein Source: UniProtKB-KW
Cellular Location
Endoplasmic reticulum membrane
Topology
Cytoplasmic: 1-23
Helical: 24-44
Lumenal: 45-458
PTM
Auto-AMPylated in vitro; it is unclear whether auto-AMPylation is relevant in vivo.
N-glycosylated; predominantly glycosylated at Asn-275.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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