FICD

FICD (FIC Domain Containing) is a Protein Coding gene. Diseases associated with FICD include Depersonalization Disorder and Extragonadal Germ Cell Cancer. Gene Ontology (GO) annotations related to this gene include protein adenylyltransferase activity.

Full Name

FIC Domain Containing

Function

Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context (By similarity).

The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place (By similarity).

Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP (PubMed:25601083).

In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it (By similarity).

In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity (By similarity).

Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates (PubMed:19362538, PubMed:25601083).

Biological Process

Negative regulation of GTPase activity Source: UniProtKB
Protein adenylylation Source: UniProtKB
Protein deadenylylation Source: UniProtKB
Regulation of IRE1-mediated unfolded protein response Source: UniProtKB
Response to endoplasmic reticulum stress Source: UniProtKB
Response to unfolded protein Source: UniProtKB-KW

Cellular Location

Endoplasmic reticulum membrane

Topology

Cytoplasmic: 1-23
Helical: 24-44
Lumenal: 45-458

PTM

Auto-AMPylated in vitro; it is unclear whether auto-AMPylation is relevant in vivo.
N-glycosylated; predominantly glycosylated at Asn-275.