Mouse Anti-FICD Recombinant Antibody (CBFYH-0197) (V2LY-0925-LY1963)

Name: Mouse Anti-FICD Recombinant Antibody (CBFYH-0197)
SKU: V2LY-0925-LY1963
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYH-0197

Application

ELISA, WB

Immunogen

Full-length recombinant FICD.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

FIC Domain Containing

Entrez Gene ID

11153

UniProt ID

Q9BVA6

Function

Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context (By similarity).

The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place (By similarity).

Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP (PubMed:25601083).

In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it (By similarity).

In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity (By similarity).

Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates (PubMed:19362538, PubMed:25601083).

Biological Process

Negative regulation of GTPase activity Source: UniProtKB
Protein adenylylation Source: UniProtKB
Protein deadenylylation Source: UniProtKB
Regulation of IRE1-mediated unfolded protein response Source: UniProtKB
Response to endoplasmic reticulum stress Source: UniProtKB
Response to unfolded protein Source: UniProtKB-KW

Cellular Location

Endoplasmic reticulum membrane

Topology

Cytoplasmic: 1-23
Helical: 24-44
Lumenal: 45-458

PTM

Auto-AMPylated in vitro; it is unclear whether auto-AMPylation is relevant in vivo.
N-glycosylated; predominantly glycosylated at Asn-275.

Perera, L. (2021). Post-translational regulation of BiP by FICD-mediated AMPylation and deAMPylation (Doctoral dissertation, University of Cambridge).

Fauser, J., Gulen, B., Pogenberg, V., Pett, C., Pourjafar-Dehkordi, D., Krisp, C., ... & Itzen, A. (2021). Specificity of AMPylation of the human chaperone BiP is mediated by TPR motifs of FICD. Nature communications, 12(1), 1-14.

Perera, L. A., Preissler, S., Zaccai, N. R., Prévost, S., Devos, J. M., Haertlein, M., & Ron, D. (2021). Structures of a deAMPylation complex rationalise the switch between antagonistic catalytic activities of FICD. Nature communications, 12(1), 1-18.

Camara, A., George, A., Hebner, E., Mahmood, A., Paluru, J., & Mattoo, S. (2020). A Fluorescence Polarization-Based High-Throughput Screen to Identify the First Small-Molecule Modulators of the Human Adenylyltransferase HYPE/FICD. International journal of molecular sciences, 21(19), 7128.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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