Mouse Anti-FICD Recombinant Antibody (CBXF-3030) (V2LY-0925-LY1964)

Name: Mouse Anti-FICD Recombinant Antibody (CBXF-3030)
SKU: V2LY-0925-LY1964
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXF-3030

Application

Immunogen

Human recombinant protein fragment corresponding to amino acids 61-265 of human FICD.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

Application	Note
WB	1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS & BSA & Glycerol

Preservative

Sodium azide

Concentration

1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

FIC Domain Containing

Entrez Gene ID

11153

UniProt ID

Q9BVA6

Function

Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context (By similarity).

The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place (By similarity).

Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP (PubMed:25601083).

In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it (By similarity).

In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity (By similarity).

Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates (PubMed:19362538, PubMed:25601083).

Biological Process

Negative regulation of GTPase activity Source: UniProtKB
Protein adenylylation Source: UniProtKB
Protein deadenylylation Source: UniProtKB
Regulation of IRE1-mediated unfolded protein response Source: UniProtKB
Response to endoplasmic reticulum stress Source: UniProtKB
Response to unfolded protein Source: UniProtKB-KW

Cellular Location

Endoplasmic reticulum membrane

Topology

Cytoplasmic: 1-23
Helical: 24-44
Lumenal: 45-458

PTM

Auto-AMPylated in vitro; it is unclear whether auto-AMPylation is relevant in vivo.
N-glycosylated; predominantly glycosylated at Asn-275.

Perera, L. (2021). Post-translational regulation of BiP by FICD-mediated AMPylation and deAMPylation (Doctoral dissertation, University of Cambridge).

Fauser, J., Gulen, B., Pogenberg, V., Pett, C., Pourjafar-Dehkordi, D., Krisp, C., ... & Itzen, A. (2021). Specificity of AMPylation of the human chaperone BiP is mediated by TPR motifs of FICD. Nature communications, 12(1), 1-14.

Perera, L. A., Preissler, S., Zaccai, N. R., Prévost, S., Devos, J. M., Haertlein, M., & Ron, D. (2021). Structures of a deAMPylation complex rationalise the switch between antagonistic catalytic activities of FICD. Nature communications, 12(1), 1-18.

Camara, A., George, A., Hebner, E., Mahmood, A., Paluru, J., & Mattoo, S. (2020). A Fluorescence Polarization-Based High-Throughput Screen to Identify the First Small-Molecule Modulators of the Human Adenylyltransferase HYPE/FICD. International journal of molecular sciences, 21(19), 7128.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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