Mouse Anti-CSPG4 Recombinant Antibody (CBFYM-1050) (CBMAB-M1203-FY)

Name: Mouse Anti-CSPG4 Recombinant Antibody (CBFYM-1050)
SKU: CBMAB-M1203-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CSPG4 Recombinant Antibody (CBFYM-1050) (CBMAB-M1203-FY) in IF

Co-staining of CSPG4 (red) and CXCL1 (green) in the kidney of wt and db/db mouse and in the kidney of normal human and DN patient. Scale bar: 10 μm for kidney in mice and 40 μm for kidney in humans. ...View More

Xu, Y., Xiang, Z., E, W., Lang, Y., Huang, S., Qin, W., ... & Liu, Z. (2023). Single-cell transcriptomes reveal a molecular link between diabetic kidney and retinal lesions. Communications Biology, 6(1), 912. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CSPG4 Recombinant Antibody (CBFYM-1050) (CBMAB-M1203-FY) in IF

Immunofluorescence staining of CSPG4 (green), PDGFRB (red) and DAPI (blue). Colocalization of CSPG4 and PDGFRB confirmed the specific expression of CSPG4 in MCs and RPCs. Scale bar: 10 μm for glomeruli and 40 μm for retinal vessels. ...View More

Mouse Anti-CSPG4 Recombinant Antibody (CBFYM-1050) (CBMAB-M1203-FY) in WB

Western blots showing the levels of CNPase, MBP and NG2 in the mouse hippocampus. ...View More

Chao, F. L., Zhang, Y., Zhang, L., Jiang, L., Zhou, C. N., Tang, J., ... & Tang, Y. (2021). Fluoxetine promotes hippocampal oligodendrocyte maturation and delays learning and memory decline in APP/PS1 mice. Frontiers in aging neuroscience, 12, 627362. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CSPG4 Recombinant Antibody (CBFYM-1050) (CBMAB-M1203-FY) in WB

Western blots showing that active chondroitinase is secreted following transduction of tissue culture cells with the lentiviral vectors. Neu7 conditioned medium (a source of CSPGs) was placed on transfected cells for 24 h, then analyzed by western blotting. ...View More

Zhao, R. R., Muir, E. M., Alves, J. N., Rickman, H., Allan, A. Y., Kwok, J. C., ... & Rogers, J. H. (2011). Lentiviral vectors express chondroitinase ABC in cortical projections and promote sprouting of injured corticospinal axons. Journal of neuroscience methods, 201(1), 228-238. Distributed under Open Access license CC BY 3.0, without modification.

Mouse Anti-CSPG4 Recombinant Antibody (CBFYM-1050) (CBMAB-M1203-FY) in WB

Chondroitinase ABC with reduced N-glycosylation is secreted in active form from transfected cells. Neu7 conditioned medium (a source of CSPGs) was placed on transfected cells for 24 h, then analysed by Western blotting. ...View More

Muir, E. M., Fyfe, I., Gardiner, S., Li, L., Warren, P., Fawcett, J. W., ... & Rogers, J. H. (2010). Modification of N-glycosylation sites allows secretion of bacterial chondroitinase ABC from mammalian cells. Journal of biotechnology, 145(2), 103-110. Distributed under Open Access license CC BY 3.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-1050

Application

WB, IP, IF, IHC-P

Immunogen

HEK293 cells expressing a truncated integral membrain form of NG2 of amino acids 1592-2222.

Host Species

Mouse

Specificity

Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

More Infomation

Target

Full Name

Chondroitin Sulfate Proteoglycan 4

Entrez Gene ID

1464

UniProt ID

Q6UVK1

Function

Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.

Biological Process

Activation of MAPK activity Source: Ensembl
Angiogenesis Source: UniProtKB-KW
Cell population proliferation Source: Ensembl
Chondroitin sulfate biosynthetic process Source: Reactome
Chondroitin sulfate catabolic process Source: Reactome
Dermatan sulfate biosynthetic process Source: Reactome
Glial cell migration Source: Ensembl
Intracellular signal transduction Source: UniProtKB
Platelet-derived growth factor receptor signaling pathway Source: Ensembl
Positive regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
Ruffle assembly Source: Ensembl
Substrate-dependent cell migration Source: Ensembl
Tissue remodeling Source: UniProtKB-KW

Cellular Location

Cell membrane; Apical cell membrane; Lamellipodium membrane; Cell surface. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes (By similarity).

Topology

Extracellular: 30-2224
Helical: 2225-2245
Cytoplasmic: 2246-2322

PTM

O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.

Uranowska, K., Kalic, T., Valtsanidis, V., Kitzwögerer, M., Breiteneder, H., & Hafner, C. (2021). Expression of chondroitin sulfate proteoglycan 4 (CSPG4) in melanoma cells is downregulated upon inhibition of BRAF. Oncology Reports, 45(4), 1-1.

Uranowska, K., Samadaei, M., Kalic, T., Pinter, M., Breiteneder, H., & Hafner, C. (2021). A chondroitin sulfate proteoglycan 4‑specific monoclonal antibody inhibits melanoma cell invasion in a spheroid model. International journal of oncology, 59(3), 1-12.

Hoffmann, R. M., Crescioli, S., Mele, S., Sachouli, E., Cheung, A., Chui, C. K., ... & Karagiannis, S. N. (2020). A novel antibody-drug conjugate (ADC) delivering a DNA mono-alkylating payload to chondroitin sulfate proteoglycan (CSPG4)-expressing melanoma. Cancers, 12(4), 1029.

Henkel, D., Tatge, H., Schöttelndreier, D., Tao, L., Dong, M., & Gerhard, R. (2020). Receptor binding domains of TcdB from Clostridioides difficile for chondroitin sulfate proteoglycan-4 and frizzled proteins are functionally independent and additive. Toxins, 12(12), 736.

Ferrone, S., & Whiteside, T. L. (2020). Targeting CSPG4 for isolation of melanoma cell-derived exosomes from body fluids. HNO, 68(2), 100-105.

Kasten, B. B., Oliver, P. G., Kim, H., Fan, J., Ferrone, S., Zinn, K. R., & Buchsbaum, D. J. (2018). 212Pb-labeled antibody 225.28 targeted to chondroitin sulfate proteoglycan 4 for triple-negative breast cancer therapy in mouse models. International journal of molecular sciences, 19(4), 925.

Schiffer, D., Mellai, M., Boldorini, R., Bisogno, I., Grifoni, S., Corona, C., ... & Annovazzi, L. (2018). The significance of chondroitin sulfate proteoglycan 4 (CSPG4) in human gliomas. International Journal of Molecular Sciences, 19(9), 2724.

Ilieva, K. M., Cheung, A., Mele, S., Chiaruttini, G., Crescioli, S., Griffin, M., ... & Karagiannis, S. N. (2018). Chondroitin sulfate proteoglycan 4 and its potential as an antibody immunotherapy target across different tumor types. Frontiers in Immunology, 8, 1911.

Hsu, S. H. C., Nadesan, P., Puviindran, V., Stallcup, W. B., Kirsch, D. G., & Alman, B. A. (2018). Effects of chondroitin sulfate proteoglycan 4 (NG2/CSPG4) on soft-tissue sarcoma growth depend on tumor developmental stage. Journal of Biological Chemistry, 293(7), 2466-2475.

Pellegatta, S., Savoldo, B., Di Ianni, N., Corbetta, C., Chen, Y., Patané, M., ... & Dotti, G. (2018). Constitutive and TNFα-inducible expression of chondroitin sulfate proteoglycan 4 in glioblastoma and neurospheres: Implications for CAR-T cell therapy. Science translational medicine, 10(430), eaao2731.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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