Mouse Anti-EMP3 Recombinant Antibody (CBFYE-0100) (CBMAB-E0207-FY)

Name: Mouse Anti-EMP3 Recombinant Antibody (CBFYE-0100)
SKU: CBMAB-E0207-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-EMP3 Recombinant Antibody (CBFYE-0100) (CBMAB-E0207-FY) in IHC

Representation of IHC images and quantification of EMP3 expression in low-grade glioma and high-grade glioma. ...View More

Zhang, A., Xu, H., Zhang, Z., Liu, Y., Han, X., Yuan, L., ... & Zhang, J. (2021). Establishment of a nomogram with EMP3 for predicting clinical outcomes in patients with glioma: A bi-center study. CNS Neuroscience & Therapeutics, 27(10), 1238-1250.

Mouse Anti-EMP3 Recombinant Antibody (CBFYE-0100) (CBMAB-E0207-FY) in IHC

Representative histologic images of hematoxylin and eosin staining and immunohistochemical staining for EMP1, EMP2, and EMP3 in phyllodes tumors with different histologic grades.

The expression of EMPs is the strongest in the epithelial component of benign phyllodes tumors (PT). Notably, strong stromal expression of EMP1, EMP2, and EMP3 is observed in malignant PT. ...View More

Cha, Y. J., & Koo, J. S. (2020). Expression of EMP1, EMP2, and EMP3 in breast phyllodes tumors. PloS one, 15(8), e0238466.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0100

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Recombinant human EMP-3.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Epitope

AA 1-163

More Infomation

Target

Full Name

epithelial membrane protein 3

Introduction

The protein encoded by this gene belongs to the PMP-22/EMP/MP20 family of proteins. The protein contains four transmembrane domains and two N-linked glycosylation sites. It is thought to be involved in cell proliferation, cell-cell interactions and function as a tumor suppressor. Alternative splicing results in multiple transcript variants.

Entrez Gene ID

2014

UniProt ID

P54852

Alternative Names

Epithelial Membrane Protein 3; Hematopoietic Neural Membrane Protein 1; HNMP-1; EMP-3; YMP; Protein YMP

Research Area

Probably involved in cell proliferation and cell-cell interactions.

Biological Process

Bleb assembly Source: UniProtKB
Cell death Source: UniProtKB

Cellular Location

Membrane

Chen, Q., Jin, J., Huang, X., Wu, F., Huang, H., & Zhan, R. (2021). EMP3 mediates glioblastoma‐associated macrophage infiltration to drive T cell exclusion. Journal of Experimental & Clinical Cancer Research, 40(1), 1-13.

Kahm, Y. J., Kim, R. K., Jung, U., & Kim, I. G. (2021). Epithelial membrane protein 3 regulates lung cancer stem cells via the TGF‑β signaling pathway. International Journal of Oncology, 59(4), 1-9.

Martija, A. A., & Pusch, S. (2021). The Multifunctional Role of EMP3 in the Regulation of Membrane Receptors Associated with IDH-Wild-Type Glioblastoma. International Journal of Molecular Sciences, 22(10), 5261.

Zhou, K., Sun, Y., Dong, D., Zhao, C., & Wang, W. (2021). EMP3 negatively modulates breast cancer cell DNA replication, DNA damage repair, and stem-like properties. Cell death & disease, 12(9), 1-10.

Li, S., Peng, H., & Li, J. (2020). MiR-765 Regulates Breast Cancer Cell Proliferation, Apoptosis, and Adriamycin Resistance Through Targeting Epithelial Membrane Protein 3. Journal of Biomaterials and Tissue Engineering, 10(8), 1193-1198.

Christians, A., Poisel, E., Hartmann, C., von Deimling, A., & Pusch, S. (2019). Characterization of the epithelial membrane protein 3 interaction network reveals a potential functional link to mitogenic signal transduction regulation. International journal of cancer, 145(2), 461-473.

Ma, Q., Zhang, Y., Liang, H., Zhang, F., Liu, F., Chen, S., ... & Liu, Y. (2018). EMP3, which is regulated by miR-663a, suppresses gallbladder cancer progression via interference with the MAPK/ERK pathway. Cancer letters, 430, 97-108.

Kusumoto, Y., Okuyama, H., Shibata, T., Konno, K., Takemoto, Y., Maekawa, D., ... & Tomura, M. (2018). Epithelial membrane protein 3 (Emp3) downregulates induction and function of cytotoxic T lymphocytes by macrophages via TNF-α production. Cellular Immunology, 324, 33-41.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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