EMP3 Antibodies

Background

EMP3 (epithelial membrane protein 3) is a fourfold transmembrane glycoprotein, mainly expressed in the nervous system of vertebrates and various epithelial tissues. This protein is involved in regulating cell proliferation, differentiation and migration, and plays a role in maintaining the stability of cell membranes and intercellular signal transduction. Studies have shown that EMP3 is often abnormally expressed in tumors such as glioma, which may be related to the occurrence, development and prognosis of tumors. Since its function was initially revealed, EMP3 has become an important research object in the cell membrane protein family. The exploration of its structure and function has provided important clues for understanding the regulation of the cellular microenvironment, the biological behavior of tumors, and the molecular mechanisms of related diseases.

Structure Function Application Advantage Our Products

Structure of EMP3

The EMP3 gene encodes a fourfold transmembrane glycoprotein with a molecular weight of approximately 18-22 kDa. Its molecular weight may fluctuate slightly among different species and tissues due to differences in the degree of glycosylation modification. The following are typical data from some research models:

Species	Human	Mouse	Rat
Molecular Weight (kDa)	About 18-22	About 18-21	About 19-22
Primary Structural Differences	Contains four transmembrane domain structure, N and C terminal are located in intracellular	Across the membrane area highly conservative, extracellular loop glycosylation sites	Sequence and human EMP3 homology is high, the function is similar

This protein is composed of 163 amino acids. Its primary structure folds to form four tight transmembrane α -helices, which constitute its core structure. Its secondary structure is mainly composed of these transmembrane helices, forming a compact bundle structure on the cell membrane. There are N-linked glycosylation sites on the two extracellular loops (such as Asn-X-Ser/Thr sequences), and these glycochain modifications are crucial for the stability of the protein, membrane localization, and interaction with other cells. The key functional domains are located in their intracellular regions and are involved in mediating intracellular signal transduction processes.

Fig. 1 Representation of EMP3′s protein structure.¹

Key structural properties of EMP3:

Four-time transmembrane α -helical bundle structure
Hydrophobic across membrane area form stable membrane anchor core
Glycosylation modifications on the extracellular ring mediate cell recognition
Intracellular domains are involved in the regulation of signal transduction

Functions of EMP3

The EMP3 gene encodes epithelial membrane protein 3, whose main function is to act as a cell membrane organizer to participate in intercellular communication and signal transduction. It also plays a complex role in neurodevelopment and tumor progression.

Function	Description
Regulation of cell adhesion and migration	By interacting with other membrane proteins, it regulates intercellular connections and cell movement, influencing the stability of tissue structure and the plasticity of cells.
Regulation of cell proliferation and differentiation	Involved in the regulation of cell growth cycle and specialized process, its abnormal expression is often associated with the disorder of cell growth.
Nervous system development	Expressed in nervous system, and participate in myelination, axon guidance and the function of glial cells.
Tumor suppressive effect	In a wide variety of cancer (such as glioma) plays a potential tumor suppressor gene function and its expression cut or silence may be associated with the occurrence and worse prognosis of tumor.
Membrane domain organization	Assist in the formation of specific lipid rafts or membrane microdomains, thereby influencing receptor aggregation and the activation efficiency of downstream signaling pathways.

Unlike some single-function proteins, the action of EMP3 is highly dependent on cell type and microenvironment, which explains the diversity and complexity of its performance under different physiological and pathological backgrounds.

Applications of EMP3 and EMP3 Antibody in Literature

1. Martija, Antoni Andreu, and Stefan Pusch. "The multifunctional role of EMP3 in the regulation of membrane receptors associated with IDH-wild-type glioblastoma." International journal of molecular sciences 22.10 (2021): 5261. https://doi.org/10.3390/ijms22105261

Epithelial membrane protein 3 (EMP3) is highly expressed in IDH wild-type glioblastoma and is a poor prognostic factor. This article reviews how EMP3 regulates oncogenic membrane receptors and explores its mechanism of action and unsolved issues in tumors.

2. Martija, Antoni Andreu, et al. "EMP3 sustains oncogenic EGFR/CDK2 signaling by restricting receptor degradation in glioblastoma." Acta neuropathologica communications 11.1 (2023): 177. https://doi.org/10.1186/s40478-023-01673-z

Studies have revealed that EMP3 maintains the malignant progression of glioblastoma by regulating EGFR transport and stability, enhancing the EGFR/CDK2 signaling pathway, and leading to drug resistance of tumor cells to targeted inhibitors.

3. Li, Li, et al. "EMP3 as a prognostic biomarker correlates with EMT in GBM." BMC cancer 24.1 (2024): 89. https://doi.org/10.1186/s12885-023-11796-0

Studies have revealed that EMP3 is highly expressed in high-grade gliomas and can serve as an independent prognostic marker. It enhances the proliferation and migration ability of glioblastoma cells by promoting epithelial-mesenchymal transition (EMT).

4. Zhou, Kailing, et al. "EMP3 negatively modulates breast cancer cell DNA replication, DNA damage repair, and stem-like properties." Cell death & disease 12.9 (2021): 844. https://doi.org/10.1038/s41419-021-04140-6

Studies have revealed that EMP3 exerts a tumor suppressor effect in breast cancer by inhibiting cell cycle progression, Akt-mTOR signaling and DNA damage repair, and down-regulating YTHDC1, thereby enhancing the tumor's chemosensitivity to doxorubicin.

5. Chen, Qun, et al. "EMP3 mediates glioblastoma‐associated macrophage infiltration to drive T cell exclusion." Journal of Experimental & Clinical Cancer Research 40.1 (2021): 160. https://doi.org/10.1186/s13046-021-01954-2

Research has revealed that EMP3 is a key immunosuppressive factor in glioblastoma. It promotes the polarization of M2-type macrophages, inhibits T cell infiltration, creates an immunosuppressive microenvironment, and influences the efficacy of anti-PD1 therapy.

Creative Biolabs: EMP3 Antibodies for Research

Creative Biolabs specializes in the production of high-quality EMP3 antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

Custom EMP3 Antibody Development: Tailor-made solutions to meet specific research requirements.
Bulk Production: Large-scale antibody manufacturing for industry partners.
Technical Support: Expert consultation for protocol optimization and troubleshooting.
Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our EMP3 antibodies, custom preparations, or technical support, contact us at email.

Reference

Martija, Antoni Andreu, and Stefan Pusch. "The multifunctional role of EMP3 in the regulation of membrane receptors associated with IDH-wild-type glioblastoma." International journal of molecular sciences 22.10 (2021): 5261. https://doi.org/10.3390/ijms22105261