Mouse Anti-ASH1L Monoclonal Antibody (ASH5H03) (CBMAB-1372-YC)

Name: Mouse Anti-ASH1L Monoclonal Antibody (ASH5H03)
SKU: CBMAB-1372-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ASH1L Monoclonal Antibody (ASH5H03) CBMAB-1372-YC in WB

Western blot analysis of ASH1L protein levels in different cancer cell lines. The GAPDH was used as a loading control. ...View More

Yu, M., Jia, Y., Ma, Z., Ji, D., Wang, C., Liang, Y., ... & Zeng, L. (2022). Structural insight into ASH1L PHD finger recognizing methylated histone H3K4 and promoting cell growth in prostate cancer. Frontiers in Oncology, 12, 906807.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

ASH5H03

Application

IP, WB

Immunogen

Amino acids 1611-1728 of ASH1L of human origin.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Purity

>95%, as determined by SDS-PAGE analysis

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ash1 (absent, small, or homeotic)-like (Drosophila)

Introduction

ASH1L is a member of the trithorax group of transcriptional activators which contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. ASH1L is localized to many small speckles in the nucleus, and also to cell-cell tight junctions.

Entrez Gene ID

55870

UniProt ID

Q9NR48

Alternative Names

ASH1; KMT2H; MRD52; ASH1L1

Function

Histone methyltransferase specifically trimethylating 'Lys-36' of histone H3 forming H3K36me3 (PubMed:21239497).
Also monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro (By similarity).
The physiological significance of the H3K9me1 activity is unclear (By similarity).

Biological Process

Decidualization Source: Ensembl
Flagellated sperm motility Source: Ensembl
Histone H3-K36 dimethylation Source: HGNC
Negative regulation of acute inflammatory response Source: Ensembl
Negative regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
Negative regulation of MAPK cascade Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Post-embryonic development Source: Ensembl
Single fertilization Source: Ensembl
Skeletal system development Source: Ensembl
Tarsal gland development Source: Ensembl
Uterine gland development Source: Ensembl
Uterus morphogenesis Source: Ensembl

Cellular Location

Nucleus; Tight junction; Chromosome. The relevance of tight junction localization is however unclear.

Involvement in disease

Mental retardation, autosomal dominant 52 (MRD52): A form of mental retardation, a disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period.

PTM

Methylated at Gln-1220 by N6AMT1.

Cui, L. X., Tian, Y. Q., Hao, H. S., Zou, H. Y., Pang, Y. W., Zhao, S. J., ... & Du, W. H. (2021). Knockdown of ASH1L methyltransferase induced apoptosis inhibiting proliferation and H3K36 methylation in bovine cumulus cells. Theriogenology, 161, 65-73.

Zhang, C., Xu, L., Zheng, X., Liu, S., & Che, F. (2021). Role of Ash1l in Tourette syndrome and other neurodevelopmental disorders. Developmental Neurobiology, 81(2), 79-91.

Xu, B., Qin, T., Yu, J., Giordano, T. J., Sartor, M. A., & Koenig, R. J. (2020). Novel role of ASH1L histone methyltransferase in anaplastic thyroid carcinoma. Journal of Biological Chemistry, 295(26), 8834-8845.

Lee, Y., Yoon, E., Cho, S., Schmähling, S., Müller, J., & Song, J. J. (2019). Structural basis of MRG15-mediated activation of the ASH1L histone methyltransferase by releasing an autoinhibitory loop. Structure, 27(5), 846-852.

Hou, P., Huang, C., Liu, C. P., Yang, N., Yu, T., Yin, Y., ... & Xu, R. M. (2019). Structural insights into stimulation of Ash1L's H3K36 methyltransferase activity through Mrg15 binding. Structure, 27(5), 837-845.

De, I., & Müller, C. W. (2019). Unleashing the power of ASH1L methyltransferase. Structure, 27(5), 727-728.

Li, G., Ye, Z., Shi, C., Sun, L., Han, M., Zhuang, Y., ... & Wu, X. (2017). The histone methyltransferase Ash1l is required for epidermal homeostasis in mice. Scientific reports, 7(1), 1-10.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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