Rat Anti-(1-5)-α-L-Arabinan Recombinant Antibody (V2-501861) (CBMAB-XB0003-YC)

Name: Rat Anti-(1-5)-α-L-Arabinan Recombinant Antibody (V2-501861)
SKU: CBMAB-XB0003-YC
Rating: 5 (1 reviews)

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Published Data

Rat Anti-(1-5)-α-L-Arabinan Recombinant Antibody (LM6) in ELISA

Monoclonal antibody binding to arabinan samples and sensitivity to enzyme action.
(a) ELISAs of monoclonal antibodies LM6, LM13, LM16 and LM17 binding to branched and de-branched samples of sugar beet arabinan, and effect of pre-treatments with an endo-arabinanase and an arabinofuranosidase. Antibodies were used to provide 90% of the maximal binding to untreated samples. Error bars indicate the SEM of three replicates.
(b) Monoclonal antibodies LM6, LM13 and LM16 binding to a branched arabinan after titration of arabinofuranosidase action. Lines indicate means of three replicates (SEM < 0.1 absorbance units in all cases). ...View More

Verhertbruggen, Y., Marcus, S. E., Haeger, A., Verhoef, R., Schols, H. A., McCleary, B. V., ... & Paul Knox, J. (2009). Developmental complexity of arabinan polysaccharides and their processing in plant cell walls. The Plant Journal, 59(3), 413-425.

Rat Anti-(1-5)-α-L-Arabinan Recombinant Antibody (LM6) in IF

Indirect immunofluorescence detection of LM6, LM13, LM16 and LM17 arabinan-related epitopes at the surface of intact Arabidopsis seedling roots. Arrowheads indicate the distal extent of root apices. The boxed area in the LM16 micrograph indicates a region of immunofluorescence revealed by longer exposure time. Scale bars = 100 μm. ...View More

Datasheet

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Basic Information

Host Animal

Rat

Clone

V2-501861

Application

ELISA, IF

Immunogen

Arabinoheptaose-BSA

Host Species

Rat

Specificity

Plant

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF	1:10

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Preservative

0.05% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

(1-5)-α-L-Arabinan

Introduction

Arabinans are side chains of the complex rhamnogalacturonan I found in the hemicellulosic part of dicotyledonous cell walls also called pectins. Arabinans can also be found as a minor component of the mycobacterial cell walls.

Alternative Names

(1-5)-α-L-Arabinan

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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