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Mouse Anti-ACTN4 Recombinant Antibody (4D10) (CBMAB-0020CQ)

This product is a mouse antibody that recognizes ACTN4. The antibody 4D10 can be used for immunoassay techniques such as: WB, ELISA, IF, IHC-P.
Host Species
Mouse
Application
WB, ELISA, IF, IHC-P
Application Notes
This 4D10 antibody has been reported in applications of WB, IF, IHC.

For western blot, the recommended concentration is 1-5 µg/ml. The band is around 100 KDa.

For IF and IHC, the reported dilution is 1:100, and concentration is 20 µg/ml.

For ELISA, this 4D10 antibody can be used as capture Ab, and recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volumn.

It's recommended that the optimal antibody concentration, dilution, incubation time etc. are best to be carefully titrated and determined by the end user.
Immunogen
Human ACTN4 (aa 592 -702)
Antibody Isotype
IgG2a
Specificity
Human, Mouse, Rat
Clone
4D10
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Introduction
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. Alpha-actinin is highly expressed in the glomerular podocyte, is important in nonmuscle cytoskeletal function, and is upregulated early in the course of some animal models of nephrotic syndrome. Using immunohistochemistry, El-Husseini et al. (2000) showed that rat RNF22 and ACTN4 are expressed in a punctate pattern throughout the cytoplasm and neuritic processes of differentiated PC12 cells. They concluded that the isoform of ACTN4 they identified may act to anchor microfilaments to various cellular structures.
Alternative Name
Actinin, Alpha 4; Focal Segmental Glomerulosclerosis 1; Non-Muscle Alpha-Actinin 4; F-Actin Cross-Linking Protein; Actinin Alpha4 Isoform; ACTININ-4; FSGS1; FSGS
Entrez Gene ID
Human81
Mouse60595
Rat63836
UniProt ID
HumanO43707
MouseP57780
RatQ9QXQ0
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For Research Use Only. Not For Clinical Use.
Cat CBMAB-0020CQ
Conjugates
Size
Price $
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Second antibody and isotype control