Mouse Anti-ACTN4 Recombinant Antibody (V2-6075) (CBMAB-0020CQ)

Name: Mouse Anti-ACTN4 Recombinant Antibody (V2-6075)
SKU: CBMAB-0020CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ACTN4 Recombinant Antibody (4D10) (CBMAB-0020CQ) in IHC

IHC of FFPE tissue on consecutive slides of normal pancreatic tissue (panel A-C) and pNEN (panel D-F), small inserts show the corresponding negative controls. Staining for Actn-4sv (A, D), CgA (B, E), and Syn (C, F). In normal pancreas the islet cells are stained for CgA and Syn but not for Actn-4sv. In contrast in pNEN a strong staining for the three markers is visible. (Magnification x 200) ...View More

Xu, X., Honda, K., Miura, N., Hori, S., Le Blanc, S., Bergmann, F., ... & Felix, K. (2020). Actinin-4 splice variant-a complementary diagnostic and prognostic marker of pancreatic neuroendocrine neoplasms. Journal of Cancer, 11(8), 2318.

Mouse Anti-ACTN4 Recombinant Antibody (4D10) (CBMAB-0020CQ) in WB

Western blot showing the knocked down expression of Diaph, Actn4, Rac1 and Cdc42 in MA104 cells transfected with the indicated siRNA, using vimentin (Vim) as loading control. The reported value indicates the mean fold-expression protein relative to the loading control, which was taken as 1. ...View More

Trejo-Cerro, O., Aguilar-Hernández, N., Silva-Ayala, D., López, S., & Arias, C. F. (2019). The actin cytoskeleton is important for rotavirus internalization and RNA genome replication. Virus research, 263, 27-33.

Mouse Anti-ACTN4 Recombinant Antibody (4D10) (CBMAB-0020CQ) in IHC

Representative actinin-4 protein expression in the epithelia of alveoli (A) and in adenocarcinoma of the lung (B and C), as determined using immunohistochemistry (IHC). Tissues negative (B) or positive (C) for actiin-4 protein expression are shown. ...View More

Shiraishi, H., Fujiwara, Y., Kakuya, T., Tsuta, K., Motoi, N., Miura, N., ... & Honda, K. (2017). Actinin-4 protein overexpression as a predictive biomarker in adjuvant chemotherapy for resected lung adenocarcinoma. Biomarkers in Medicine, 11(9), 721-731.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-6075

Application

WB, ELISA, IF, IHC-P

Immunogen

Human ACTN4 (aa 592 -702)

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

This 4D10 antibody has been reported in applications of WB, IF, IHC.
For western blot, the recommended concentration is 1-5 µg/ml. The band is around 100 KDa.
For IF and IHC, the reported dilution is 1:100, and concentration is 20 µg/ml.
For ELISA, this 4D10 antibody can be used as capture Ab, and recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volumn.
It's recommended that the optimal antibody concentration, dilution, incubation time etc. are best to be carefully titrated and determined by the end user.
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC-P	3 μg/ml
IF(ICC)	20 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Actinin Alpha 4

Introduction

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. Alpha-actinin is highly expressed in the glomerular podocyte, is important in nonmuscle cytoskeletal function, and is upregulated early in the course of some animal models of nephrotic syndrome. Using immunohistochemistry, El-Husseini et al. (2000) showed that rat RNF22 and ACTN4 are expressed in a punctate pattern throughout the cytoplasm and neuritic processes of differentiated PC12 cells. They concluded that the isoform of ACTN4 they identified may act to anchor microfilaments to various cellular structures.

Entrez Gene ID

Human	81
Mouse	60595
Rat	63836

UniProt ID

Human	O43707
Mouse	P57780
Rat	Q9QXQ0

Alternative Names

Actinin, Alpha 4; Focal Segmental Glomerulosclerosis 1; Non-Muscle Alpha-Actinin 4; F-Actin Cross-Linking Protein; Actinin Alpha4 Isoform; ACTININ-4; FSGS1; FSGS

Function

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA.

Biological Process

Negative regulation of substrate adhesion-dependent cell spreading
Peroxisome proliferator activated receptor signaling pathway
Platelet degranulation
Positive regulation of cell migration
Positive regulation of cellular component movement
Positive regulation of NIK/NF-kappaB signaling
Positive regulation of sodium:proton antiporter activity
Protein transport
Regulation of apoptotic process
Regulation of nucleic acid-templated transcription
Response to hypoxia
Retinoic acid receptor signaling pathway
Tumor necrosis factor-mediated signaling pathway
Vesicle transport along actin filament

Cellular Location

Nucleus; Cytoplasm; Stress fiber; Cell junction. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines (breast cancer cell line MCF-7, oral floor cancer IMC-2, and bladder cancer KU-7).

Involvement in disease

Focal segmental glomerulosclerosis 1 (FSGS1): A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and progressive decline in renal function. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.

Shao, H., & Wells, A. (2021). Binding of alpha-ACTN4 to EGF receptor enables its rapid phosphorylation. Heliyon, 7(1), e06011.

Xing, Z., Li, S., Liu, Z., Zhang, C., & Bai, Z. (2020). CTCF‐induced upregulation of HOXA11‐AS facilitates cell proliferation and migration by targeting miR‐518b/ACTN4 axis in prostate cancer. The Prostate, 80(5), 388-398.

Ishizuya, Y., Uemura, M., Narumi, R., Tomiyama, E., Koh, Y., Matsushita, M., ... & Nonomura, N. (2020). The role of actinin-4 (ACTN4) in exosomes as a potential novel therapeutic target in castration-resistant prostate cancer. Biochemical and biophysical research communications, 523(3), 588-594.

Lin, Y. H., Huang, C. Y., Ke, C. C., Wang, Y. Y., Lai, T. H., Liu, H. C., ... & Lin, Y. H. (2020). ACTN4 mediates SEPT14 mutation-induced sperm head defects. Biomedicines, 8(11), 518.

Tentler, D., Lomert, E., Novitskaya, K., & Barlev, N. A. (2019). Role of ACTN4 in Tumorigenesis, Metastasis, and EMT. Cells, 8(11), 1427.

Ji, J., Xu, R., Ding, K., Bao, G., Zhang, X., Huang, B., ... & Wang, J. (2019). Long noncoding RNA SChLAP1 forms a growth-promoting complex with HNRNPL in human glioblastoma through stabilization of ACTN4 and activation of NF-κB signaling. Clinical Cancer Research, 25(22), 6868-6881.

Zhao, J., Peng, W., Ran, Y., Ge, H., Zhang, C., Zou, H., ... & Qi, H. (2019). Dysregulated expression of ACTN4 contributes to endothelial cell injury via the activation of the p38-MAPK/p53 apoptosis pathway in preeclampsia. Journal of physiology and biochemistry, 75(4), 475-487.

Zhang, Y. Y., Tabataba, H., Liu, X. Y., Wang, J. Y., Yan, X. G., Farrelly, M., ... & Jin, L. (2018). ACTN4 regulates the stability of RIPK1 in melanoma. Oncogene, 37(29), 4033-4045.

Lomert, E., Turoverova, L., Kriger, D., Aksenov, N. D., Nikotina, A. D., Petukhov, A., ... & Tentler, D. (2018). Co-expression of RelA/p65 and ACTN4 induces apoptosis in non-small lung carcinoma cells. Cell Cycle, 17(5), 616-626.

Kakuya, T., Mori, T., Yoshimoto, S., Watabe, Y., Miura, N., Shoji, H., ... & Honda, K. (2017). Prognostic significance of gene amplification of ACTN4 in stage I and II oral tongue cancer. International journal of oral and maxillofacial surgery, 46(8), 968-976.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

ACTN4 Matched Antibody Pair (011) (CAT#: APMAB-011LY)

ACTN4 Matched Antibody Pair (012) (CAT#: APMAB-012LY)

ACTN4 Matched Antibody Pair (013) (CAT#: APMAB-013LY)

ACTN4 Matched Antibody Pair (014) (CAT#: APMAB-014LY)

ACTN4 Matched Antibody Pair (015) (CAT#: APMAB-015LY)

ACTN4 Matched Antibody Pair (016) (CAT#: APMAB-016LY)

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For research use only. Not intended for any clinical use.

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