Mouse Anti-CD247 Recombinant Antibody (6B10.2) (CBMAB-C1583-YY)

Name: Mouse Anti-CD247 Recombinant Antibody (6B10.2)
SKU: CBMAB-C1583-YY
Rating: 5 (1 reviews)

See all CD247 antibodies

Compare Online Inquiry

Published Data

Mouse Anti-CD247 Recombinant Antibody (6B10.2) (CBMAB-C1583-YY) in Immunoblot and IP

TCRζ was immunoprecipitated from control ZAP-70+/+ Jurkat cells (E6.1) as well as from EGFP-, ZAP-R170C/EGFP-, and ZAP-WT/EGFP-transduced ZAP-70-/- p116 cells after a 3 minute anti-CD3 mAb stimulation. Representative immunoblots showing immunoprecipitated TCRζ levels as well as ζ-associated ZAP-70 are presented (n=2 for each panel). ...View More

Mongellaz, C., Vicente, R., Noroski, L. M., Noraz, N., Courgnaud, V., Chinen, J., ... & Taylor, N. (2023). Combined immunodeficiency caused by pathogenic variants in the ZAP70 C-terminal SH2 domain. Frontiers in Immunology, 14, 1155883. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CD247 Recombinant Antibody (6B10.2) (CBMAB-C1583-YY) in Immunoblot

Jurkat cells were transfected with either siCtrl or siINPP5E. Immunoblots were performed to analyze the phosphorylation levels of indicated proteins in Ctrl or INPP5E-knockdown Jurkat T cells activated by crosslinking with anti-CD3/CD28 for the indicated times. α-tubulin and non-phosphorylated molecules were blotted as loading controls. ...View More

Chiu, T. Y., Lo, C. H., Lin, Y. H., Lai, Y. D., Lin, S. S., Fang, Y. T., ... & Wang, W. J. (2023). INPP5E regulates CD3ζ enrichment at the immune synapse by phosphoinositide distribution control. Communications Biology, 6(1), 911. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CD247 Recombinant Antibody (6B10.2) (CBMAB-C1583-YY) in IF

Jurkat cells were transfected with either siCtrl or siINPP5E. Immunostaining of pCD3ζ-Y83 (upper) and pZAP-70-Y493 (lower) in conjugates of the control or INPP5E-knockdown Jurkat T cells and CMAC-labeled SEE-pulsed APCs. Phalloidin staining was performed to visualize F-actin. Representative images are shown. ...View More

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

6B10.2

Application

WB, IP, IHC-P, FC, IF

Immunogen

Amino acids 89-105 mapping within a cytoplasmic domain of CD3-ζ of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IHC-P	1:50-1:500
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

CD247 Molecule

Entrez Gene ID

919

UniProt ID

P20963

Function

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098, PubMed:7509083).
CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (PubMed:7509083).
Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN) (By similarity).

Biological Process

Adaptive immune response Source: UniProtKB-KW
Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
Negative regulation of inflammatory response to antigenic stimulus Source: Reactome
Peptide cross-linking Source: Reactome
Positive regulation of protein localization to cell surface Source: Ensembl
Regulation of defense response to virus by virus Source: Reactome
Regulation of immune response Source: Reactome
T cell receptor signaling pathway Source: GO_Central

Cellular Location

Cell membrane

Involvement in disease

Immunodeficiency 25 (IMD25): An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.

Topology

Extracellular: 22-30
Helical: 31-51
Cytoplasmic: 52-164

PTM

Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.

Al‐Akioui‐Sanz, K., Moraru, M., & Vilches, C. (2021). A simple genotyping method for CD247 3′‐untranslated region polymorphism rs1052231 and characterization of a reference cell panel. HLA, 98(3), 218-222.

Mamoor, S. (2021). CD247 is differentially expressed in patients with metastatic breast cancer, in the lymph nodes and in the brain.

Chen, S. S., Feng, Y. Y., Ma, R. Y., & Wang, H. L. (2020). CD247 Functions as a Prognostic Biomarker for Cutaneous Malignant Melanoma Based on the Analysis of Tumor-immune Microenvironment.

Ye, W., Zhou, Y., Xu, B., Zhu, D., Rui, X., Xu, M., ... & Jiang, J. (2019). CD247 expression is associated with differentiation and classification in ovarian cancer. Medicine, 98(51).

Abbasi, F., Mansouri, R., Gharibdoost, F., Aslani, S., Mostafaei, S., Kavosi, H., ... & Mahmoudi, M. (2017). Association study of CD226 and CD247 genes single nucleotide polymorphisms in Iranian patients with systemic sclerosis. Iranian Journal of Allergy, Asthma and Immunology, 471-479.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-CD247 Recombinant Antibody (6B10.2)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-CD247 Recombinant Antibody (CBFYC-1177) (CAT#: CBMAB-C1235-FY)

Armenian Hamster Anti-CD247 Recombinant Antibody (H146-968) (CAT#: CBMAB-C0681-CQ)

Mouse Anti-CD247 Recombinant Antibody (CBFYC-0026) (CAT#: CBMAB-C0033-FY)

Rabbit Anti-CD247 Recombinant Antibody (CBYJT-1087) (CAT#: CBMAB-T0097-YJ)

Mouse Anti-CD247 Recombinant Antibody (CBXC-2911) (CAT#: CBMAB-C0231-CQ)

Mouse Anti-CD247 Recombinant Antibody (CBT294) (CAT#: V2LY-0625-LY3625)

Mouse Anti-CD247 Recombinant Antibody (CBT4450) (CAT#: V2LY-0625-LY2418)

Mouse Anti-CD247 (phosphorylated Tyr142) Recombinant Antibody (EM-54) (CAT#: PTM-CBMAB-R4117-CN)

Mouse Anti-CD247 Recombinant Antibody (CBYY-C1280) (CAT#: CBMAB-C2718-YY)

Mouse Anti-CD247 (phosphorylated Tyr153) Recombinant Antibody (EM-17) (CAT#: PTM-CBMAB-R4116-CN)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry