Human Recombinant CD247 protein (V2LY-0526-LY2777)

Name: Human Recombinant CD247 protein
SKU: V2LY-0526-LY2777
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Protein Construction

This product is Human Recombinant CD247 protein consist of Amino Acid: 52-164 and predicts a molecular mass of 13.18 kDa.

Molecule Mass

13.18 kDa

Sequence

Amino Acid: 52-164

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD247 Molecule

Function

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098, PubMed:7509083).
CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (PubMed:7509083).
Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN) (By similarity).

Biological Process

Adaptive immune response Source: UniProtKB-KW
Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
Negative regulation of inflammatory response to antigenic stimulus Source: Reactome
Peptide cross-linking Source: Reactome
Positive regulation of protein localization to cell surface Source: Ensembl
Regulation of defense response to virus by virus Source: Reactome
Regulation of immune response Source: Reactome
T cell receptor signaling pathway Source: GO_Central

Cellular Location

Cell membrane

Involvement in disease

Immunodeficiency 25 (IMD25): An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.

Topology

Extracellular: 22-30
Helical: 31-51
Cytoplasmic: 52-164

PTM

Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.

Al‐Akioui‐Sanz, K., Moraru, M., & Vilches, C. (2021). A simple genotyping method for CD247 3′‐untranslated region polymorphism rs1052231 and characterization of a reference cell panel. HLA, 98(3), 218-222.

Mamoor, S. (2021). CD247 is differentially expressed in patients with metastatic breast cancer, in the lymph nodes and in the brain.

Chen, S. S., Feng, Y. Y., Ma, R. Y., & Wang, H. L. (2020). CD247 Functions as a Prognostic Biomarker for Cutaneous Malignant Melanoma Based on the Analysis of Tumor-immune Microenvironment.

Ye, W., Zhou, Y., Xu, B., Zhu, D., Rui, X., Xu, M., ... & Jiang, J. (2019). CD247 expression is associated with differentiation and classification in ovarian cancer. Medicine, 98(51).

Abbasi, F., Mansouri, R., Gharibdoost, F., Aslani, S., Mostafaei, S., Kavosi, H., ... & Mahmoudi, M. (2017). Association study of CD226 and CD247 genes single nucleotide polymorphisms in Iranian patients with systemic sclerosis. Iranian Journal of Allergy, Asthma and Immunology, 471-479.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Cd247 ELISA Kit (CAT#: V2LY-0626-LY3119)

Human CD247 ELISA Kit (CAT#: V2LY-0626-LY3760)

Human Recombinant CD247 protein, GST Tag (CAT#: V2LY-0526-LY2778)

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Human Recombinant CD247 protein, GST Tag (CAT#: V2LY-0526-LY2778)

Mouse Anti-CD247 Recombinant Antibody (CBFYC-0026) (CAT#: CBMAB-C0033-FY)

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Mouse Anti-CD247 Recombinant Antibody (6B10.2) (CAT#: CBMAB-C1583-YY)

Mouse Anti-CD247 Recombinant Antibody (CBT294) (CAT#: V2LY-0625-LY3625)

Mouse Anti-CD247 (phosphorylated Tyr142) Recombinant Antibody (EM-54) (CAT#: PTM-CBMAB-R4117-CN)

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Mouse Anti-CD247 Recombinant Antibody (CBT291) (CAT#: V2LY-0625-LY3628)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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