Mouse Cd247 ELISA Kit (V2LY-0626-LY3119)

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Tested Data
Datasheet
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Basic Information

Sensitivity
0.014 ng/mL
Detection Range
0.03-9 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
18.6 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
CD247 Molecule
Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098, PubMed:7509083).
CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (PubMed:7509083).
Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN) (By similarity).
Biological Process
Adaptive immune response Source: UniProtKB-KW
Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
Negative regulation of inflammatory response to antigenic stimulus Source: Reactome
Peptide cross-linking Source: Reactome
Positive regulation of protein localization to cell surface Source: Ensembl
Regulation of defense response to virus by virus Source: Reactome
Regulation of immune response Source: Reactome
T cell receptor signaling pathway Source: GO_Central
Cellular Location
Cell membrane
Involvement in disease
Immunodeficiency 25 (IMD25): An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
Topology
Extracellular: 22-30
Helical: 31-51
Cytoplasmic: 52-164
PTM
Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.

Al‐Akioui‐Sanz, K., Moraru, M., & Vilches, C. (2021). A simple genotyping method for CD247 3′‐untranslated region polymorphism rs1052231 and characterization of a reference cell panel. HLA, 98(3), 218-222.

Mamoor, S. (2021). CD247 is differentially expressed in patients with metastatic breast cancer, in the lymph nodes and in the brain.

Chen, S. S., Feng, Y. Y., Ma, R. Y., & Wang, H. L. (2020). CD247 Functions as a Prognostic Biomarker for Cutaneous Malignant Melanoma Based on the Analysis of Tumor-immune Microenvironment.

Ye, W., Zhou, Y., Xu, B., Zhu, D., Rui, X., Xu, M., ... & Jiang, J. (2019). CD247 expression is associated with differentiation and classification in ovarian cancer. Medicine, 98(51).

Abbasi, F., Mansouri, R., Gharibdoost, F., Aslani, S., Mostafaei, S., Kavosi, H., ... & Mahmoudi, M. (2017). Association study of CD226 and CD247 genes single nucleotide polymorphisms in Iranian patients with systemic sclerosis. Iranian Journal of Allergy, Asthma and Immunology, 471-479.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human CD247 ELISA Kit (CAT#: V2LY-0626-LY3760)

Human Recombinant CD247 protein (CAT#: V2LY-0526-LY2777)

Human Recombinant CD247 protein, GST Tag (CAT#: V2LY-0526-LY2778)

Related Products

Mouse Anti-CD247 Recombinant Antibody (CBYY-C1280) (CAT#: CBMAB-C2718-YY)

Mouse Anti-CD247 (phosphorylated Tyr142) Recombinant Antibody (EM-54) (CAT#: PTM-CBMAB-R4117-CN)

Mouse Anti-CD247 Recombinant Antibody (CBFYC-0026) (CAT#: CBMAB-C0033-FY)

Mouse Anti-CD247 Recombinant Antibody (CBT291) (CAT#: V2LY-0625-LY3628)

Mouse Anti-CD247 Recombinant Antibody (CBT4450) (CAT#: V2LY-0625-LY2418)

Mouse Anti-CD247 Recombinant Antibody (CBYY-C0165) (CAT#: CBMAB-C0517-YY)

Mouse Anti-CD247 Recombinant Antibody (CBT295) (CAT#: V2LY-0625-LY3624)

Human Recombinant CD247 protein, GST Tag (CAT#: V2LY-0526-LY2778)

Rabbit Anti-CD247 Recombinant Antibody (
21H6) (CAT#: V2LY-0725-LY1102)

Mouse Anti-CD247 Recombinant Antibody (CBT294) (CAT#: V2LY-0625-LY3625)

Isotype control

For research use only. Not intended for any clinical use.

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