Mouse Anti-CD247 Recombinant Antibody (CBFYC-1177) (CBMAB-C1235-FY)

Name: Mouse Anti-CD247 Recombinant Antibody (CBFYC-1177)
SKU: CBMAB-C1235-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CD247 Recombinant Antibody (CBFYC-1177) (CBMAB-C1235-FY) in Immunoblot

RhoH mediates the interaction of Lck and CD3ζ.
Thymocytes (A) and lymph node (LN)-derived T cells (B) from wild type (WT) and Rhoh-/- mice were left unstimulated or stimulated with anti-CD3/28 Abs for 2 min. Lck autophosphorylation activity was measured by immune complex kinase assay with [γ-32P]ATP. Total lysates were submitted to immunoprecipitation (IP) of Lck and immunoprecipitates and total lysates were immunoblotted (IB) for CD3ζ, p-Tyr and Lck. Lck phosphorylation were analyzed by immunoblotting for anti-pY394-Lck (anti-pY416-Src) or Lck of Lck IP products. ...View More

Chae, H. D., Siefring, J. E., Hildeman, D. A., Gu, Y., & Williams, D. A. (2010). RhoH regulates subcellular localization of ZAP-70 and Lck in T cell receptor signaling. PloS one, 5(11), e13970. Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Mouse Anti-CD247 Recombinant Antibody (CBFYC-1177) (CBMAB-C1235-FY) in Immunoblot

Rhoh-/- LDBM cells were transduced with a retroviral vector co-expressing Myr-ZAP-70, HA-RhoH and EGFP or EGFP alone as a control. EGFP+-sorted cells were injected intravenously into sub-lethally irradiated Rag2-/- recipient mice. Thymocytes from Rag2-/- recipient mice were analyzed 8 weeks after transplantation immunoblotting. ...View More

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-1177

Application

WB, IP, IHC-P, FC, IF

Immunogen

Amino acids 89-105 mapping within a cytoplasmic domain of CD3-ζ of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IHC-P	1:50-1:500
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 153-164

More Infomation

Target

Full Name

CD247 Molecule

Introduction

CD247 (CD247 Molecule) is a Protein Coding gene. Diseases associated with CD247 include Immunodeficiency 25 and T-B+ Severe Combined Immunodeficiency Due To Cd3delta/Cd3epsilon/Cd3zeta. Among its related pathways are G-protein signaling N-RAS regulation pathway and Role of phospholipids in phagocytosis. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and transmembrane signaling receptor activity.

Entrez Gene ID

919

UniProt ID

P20963

Function

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098, PubMed:7509083).
CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (PubMed:7509083).
Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN) (By similarity).

Biological Process

Adaptive immune response Source: UniProtKB-KW
Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
Negative regulation of inflammatory response to antigenic stimulus Source: Reactome
Peptide cross-linking Source: Reactome
Positive regulation of protein localization to cell surface Source: Ensembl
Regulation of defense response to virus by virus Source: Reactome
Regulation of immune response Source: Reactome
T cell receptor signaling pathway Source: GO_Central

Cellular Location

Cell membrane

Involvement in disease

Immunodeficiency 25 (IMD25): An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.

Topology

Extracellular: 22-30
Helical: 31-51
Cytoplasmic: 52-164

PTM

Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.

Al‐Akioui‐Sanz, K., Moraru, M., & Vilches, C. (2021). A simple genotyping method for CD247 3′‐untranslated region polymorphism rs1052231 and characterization of a reference cell panel. HLA, 98(3), 218-222.

Mamoor, S. (2021). CD247 is differentially expressed in patients with metastatic breast cancer, in the lymph nodes and in the brain.

Chen, S. S., Feng, Y. Y., Ma, R. Y., & Wang, H. L. (2020). CD247 Functions as a Prognostic Biomarker for Cutaneous Malignant Melanoma Based on the Analysis of Tumor-immune Microenvironment.

Ye, W., Zhou, Y., Xu, B., Zhu, D., Rui, X., Xu, M., ... & Jiang, J. (2019). CD247 expression is associated with differentiation and classification in ovarian cancer. Medicine, 98(51).

Abbasi, F., Mansouri, R., Gharibdoost, F., Aslani, S., Mostafaei, S., Kavosi, H., ... & Mahmoudi, M. (2017). Association study of CD226 and CD247 genes single nucleotide polymorphisms in Iranian patients with systemic sclerosis. Iranian Journal of Allergy, Asthma and Immunology, 471-479.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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