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Mouse Anti-CD247 Recombinant Antibody (CBT294) (V2LY-0625-LY3625)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT294
Antibody Isotype
IgG
Application
FC, ELISA

Basic Information

Immunogen
Purified recombinant fragment of human CD3 expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
ELISA1:10,000
FC1:200-1:400

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
CD247 Molecule
Entrez Gene ID
919
UniProt ID
P20963
Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098, PubMed:7509083).
CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (PubMed:7509083).
Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN) (By similarity).
Biological Process
Adaptive immune response Source: UniProtKB-KW
Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
Negative regulation of inflammatory response to antigenic stimulus Source: Reactome
Peptide cross-linking Source: Reactome
Positive regulation of protein localization to cell surface Source: Ensembl
Regulation of defense response to virus by virus Source: Reactome
Regulation of immune response Source: Reactome
T cell receptor signaling pathway Source: GO_Central
Cellular Location
Cell membrane
Involvement in disease
Immunodeficiency 25 (IMD25): An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
Topology
Extracellular: 22-30
Helical: 31-51
Cytoplasmic: 52-164
PTM
Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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