Rabbit Anti-ALDOA Recombinant Antibody (D73H4) (CBMAB-A2314-YC)

Name: Rabbit Anti-ALDOA Recombinant Antibody (D73H4)
SKU: CBMAB-A2314-YC
Rating: 5 (1 reviews)

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Published Data

Rabbit Anti-ALDOA Recombinant Antibody (D73H4) in WB

Protein expression level of Aldolase A and Enolase in pTRE-D2 SCC13 cells after doxycycline treatment. Each experiment was performed in triplicates. Results are expressed as the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. ...View More

Miro, C., Nappi, A., Cicatiello, A. G., Di Cicco, E., Sagliocchi, S., Murolo, M., ... & Dentice, M. (2021). Thyroid hormone enhances angiogenesis and the Warburg effect in squamous cell carcinomas. Cancers, 13(11), 2743.

Rabbit Anti-ALDOA Recombinant Antibody (D73H4) in WB

Western blot analysis showing cycloheximide (CHX) chase experiments of JOSD2, PHGDH, Aldolase A, and PFK-1 protein degradation up to 48 h, in A549 cells following transfection with siRNA JOSD2 or control siRNA for 24 h prior CHX addition. MG132 (5 µM) was supplied for the last 4 h. The indicated enzyme levels were normalized to porin. ...View More

Krassikova, L., Zhang, B., Nagarajan, D., Queiroz, A. L., Kacal, M., Samakidis, E., ... & Norberg, E. (2021). The deubiquitinase JOSD2 is a positive regulator of glucose metabolism. Cell Death & Differentiation, 28(3), 1091-1109.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

D73H4

Application

Immunogen

Synthetic peptide corresponding to residues surrounding Pro263 of human fructose bisphosphate aldolase A protein.

Host Species

Rabbit

Specificity

Human, Mouse, Monkey

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, pH 7.5, 150 mM NaCl, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Aldolase, Fructose-Bisphosphate A

Introduction

ALDOA is a member of the class I fructose-bisphosphate aldolase protein family. The encoded protein is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate.

Entrez Gene ID

226

UniProt ID

P04075

Alternative Names

Aldolase, Fructose-Bisphosphate A; Aldolase A, Fructose-Bisphosphate; Lung Cancer Antigen NY-LU-1; Muscle-Type Aldolase; EC 4.1.2.13; ALDA;

Function

Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein (By similarity).

Biological Process

Actin filament organization Source: BHF-UCL
ATP biosynthetic process Source: BHF-UCL
Binding of sperm to zona pellucida Source: CAFA
Canonical glycolysis Source: Reactome
Fructose 1,6-bisphosphate metabolic process Source: BHF-UCL
Fructose metabolic process Source: BHF-UCL
Gluconeogenesis Source: Reactome
Glycolytic process Source: BHF-UCL
Muscle cell cellular homeostasis Source: BHF-UCL
Neutrophil degranulation Source: Reactome
Platelet degranulation Source: Reactome
Protein homotetramerization Source: UniProtKB
Regulation of cell shape Source: BHF-UCL
Striated muscle contraction Source: BHF-UCL

Cellular Location

I band; M line. In skeletal muscle, accumulates around the M line and within the I band, colocalizing with FBP2 on both sides of the Z line in the absence of Ca2+.

Involvement in disease

Glycogen storage disease 12 (GSD12): A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.

Li, X., Jiang, F., Ge, Z., Chen, B., Yu, J., Xin, M., ... & Wu, L. (2019). Fructose-Bisphosphate Aldolase A Regulates hypoxic adaptation in hepatocellular carcinoma and involved with tumor malignancy. Digestive diseases and sciences, 64(11), 3215-3227.

Huang, Z., Hua, Y., Tian, Y., Qin, C., Qian, J., Bao, M., ... & Gu, M. (2018). High expression of fructose-bisphosphate aldolase A induces progression of renal cell carcinoma. Oncology reports, 39(6), 2996-3006.

Kawai, K., Uemura, M., Munakata, K., Takahashi, H., Haraguchi, N., Nishimura, J., ... & Mori, M. (2017). Fructose-bisphosphate aldolase A is a key regulator of hypoxic adaptation in colorectal cancer cells and involved in treatment resistance and poor prognosis. International journal of oncology, 50(2), 525-534.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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