Mouse Anti-ALDOA Recombinant Antibody (CBT2236) (V2LY-0625-LY717)

Name: Mouse Anti-ALDOA Recombinant Antibody (CBT2236)
SKU: V2LY-0625-LY717
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-ALDOA Recombinant Antibody (CBT2236) (V2LY-0625-LY717) in ELISA

Mouse Anti-ALDOA Recombinant Antibody (CBT2236) (V2LY-0625-LY717) in WB

Mouse Anti-ALDOA Recombinant Antibody (CBT2236) (V2LY-0625-LY717) in IF

Mouse Anti-ALDOA Recombinant Antibody (CBT2236) (V2LY-0625-LY717) in FC

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT2236

Application

ICC, FC

Immunogen

Purified recombinant fragment of human ALDOA (AA: 9-145) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ICC	1:100-1:500
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Aldolase, Fructose-Bisphosphate A

Entrez Gene ID

226

UniProt ID

P04075

Function

Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein (By similarity).

Biological Process

Actin filament organization Source: BHF-UCL
ATP biosynthetic process Source: BHF-UCL
Binding of sperm to zona pellucida Source: CAFA
Canonical glycolysis Source: Reactome
Fructose 1,6-bisphosphate metabolic process Source: BHF-UCL
Fructose metabolic process Source: BHF-UCL
Gluconeogenesis Source: Reactome
Glycolytic process Source: BHF-UCL
Muscle cell cellular homeostasis Source: BHF-UCL
Neutrophil degranulation Source: Reactome
Platelet degranulation Source: Reactome
Protein homotetramerization Source: UniProtKB
Regulation of cell shape Source: BHF-UCL
Striated muscle contraction Source: BHF-UCL

Cellular Location

I band; M line. In skeletal muscle, accumulates around the M line and within the I band, colocalizing with FBP2 on both sides of the Z line in the absence of Ca2+.

Involvement in disease

Glycogen storage disease 12 (GSD12): A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.

Li, X., Jiang, F., Ge, Z., Chen, B., Yu, J., Xin, M., ... & Wu, L. (2019). Fructose-Bisphosphate Aldolase A Regulates hypoxic adaptation in hepatocellular carcinoma and involved with tumor malignancy. Digestive diseases and sciences, 64(11), 3215-3227.

Huang, Z., Hua, Y., Tian, Y., Qin, C., Qian, J., Bao, M., ... & Gu, M. (2018). High expression of fructose-bisphosphate aldolase A induces progression of renal cell carcinoma. Oncology reports, 39(6), 2996-3006.

Kawai, K., Uemura, M., Munakata, K., Takahashi, H., Haraguchi, N., Nishimura, J., ... & Mori, M. (2017). Fructose-bisphosphate aldolase A is a key regulator of hypoxic adaptation in colorectal cancer cells and involved in treatment resistance and poor prognosis. International journal of oncology, 50(2), 525-534.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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