Rabbit Anti-ALDOA Recombinant Antibody (
7D8) (V2LY-0725-LY1260)

Name: Rabbit Anti-ALDOA Recombinant Antibody (7D8)
SKU: V2LY-0725-LY1260
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-ALDOA Recombinant Antibody (7D8) (V2LY-0725-LY1260) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, A549 whole cell lysate, Mouse Liver whole cell lysate, Rat Liver whole cell lysate
All lanes: Aldolase antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 40, 46 kDa
Observed band size: 40 kDa

Rabbit Anti-ALDOA Recombinant Antibody (7D8) (V2LY-0725-LY1260) in IHC

IHC image of V2LY-0725-LY1260 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-ALDOA Recombinant Antibody (7D8) (V2LY-0725-LY1260) in IF

Immunofluorescence staining of Hela Cells with V2LY-0725-LY1260 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

7D8

Application

ELISA, WB, IHC, IF

Immunogen

A synthesized peptide derived from human Aldolase.

Host Species

Rabbit

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200
IF	1:20-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Aldolase, Fructose-Bisphosphate A

Entrez Gene ID

226

UniProt ID

P04075

Function

Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein (By similarity).

Biological Process

Actin filament organization Source: BHF-UCL
ATP biosynthetic process Source: BHF-UCL
Binding of sperm to zona pellucida Source: CAFA
Canonical glycolysis Source: Reactome
Fructose 1,6-bisphosphate metabolic process Source: BHF-UCL
Fructose metabolic process Source: BHF-UCL
Gluconeogenesis Source: Reactome
Glycolytic process Source: BHF-UCL
Muscle cell cellular homeostasis Source: BHF-UCL
Neutrophil degranulation Source: Reactome
Platelet degranulation Source: Reactome
Protein homotetramerization Source: UniProtKB
Regulation of cell shape Source: BHF-UCL
Striated muscle contraction Source: BHF-UCL

Cellular Location

I band; M line. In skeletal muscle, accumulates around the M line and within the I band, colocalizing with FBP2 on both sides of the Z line in the absence of Ca2+.

Involvement in disease

Glycogen storage disease 12 (GSD12): A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.

Li, X., Jiang, F., Ge, Z., Chen, B., Yu, J., Xin, M., ... & Wu, L. (2019). Fructose-Bisphosphate Aldolase A Regulates hypoxic adaptation in hepatocellular carcinoma and involved with tumor malignancy. Digestive diseases and sciences, 64(11), 3215-3227.

Huang, Z., Hua, Y., Tian, Y., Qin, C., Qian, J., Bao, M., ... & Gu, M. (2018). High expression of fructose-bisphosphate aldolase A induces progression of renal cell carcinoma. Oncology reports, 39(6), 2996-3006.

Kawai, K., Uemura, M., Munakata, K., Takahashi, H., Haraguchi, N., Nishimura, J., ... & Mori, M. (2017). Fructose-bisphosphate aldolase A is a key regulator of hypoxic adaptation in colorectal cancer cells and involved in treatment resistance and poor prognosis. International journal of oncology, 50(2), 525-534.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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