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Mouse Anti-ALDOA Recombinant Antibody (C10) (CBMAB-F1413-CQ)

This product is a mouse antibody that recognizes ALDOA. The antibody CBXF-0110 can be used for immunoassay techniques such as: WB, ICC, IHC-P, IHC-Fr, ELISA.
See all ALDOA antibodies
Published Data
Mouse Anti-ALDOA Recombinant Antibody (C10) in WB
Levels of PPAR-γ in cytosolic or nuclear extracts were determined by western blot analysis. Aldolase A is used as a marker of cytosolic marker, while lamin B1 was used as a nuclear marker (upper panel). The densitometry data represent the mean ± standard error from three immunoblots and are shown as the relative density of protein band normalized to aldolase A or lamin B1 levels (lower panel). ...View More
Lee, Y., Lim, J. W., & Kim, H. (2022). α‑lipoic acid inhibits cerulein/resistin‑induced expression of interleukin‑6 by activating peroxisome proliferator‑activated receptor‑γ in pancreatic acinar cells. Molecular Medicine Reports, 26(2), 1-11.
Mouse Anti-ALDOA Recombinant Antibody (C10) in WB
Transcript and protein expression levels of ALDOA, ENO1, GPI, HK2, and PGK1 in 143B cells with overexpression or knockdown of RBM15 and circ-CTNNB1 measured by western blot assay. ...View More
Yang, F., Liu, Y., Xiao, J., Li, B., Chen, Y., Hu, A., ... & Liu, H. (2023). Circ-CTNNB1 drives aerobic glycolysis and osteosarcoma progression via m6A modification through interacting with RBM15. Cell proliferation, 56(1), e13344.
Mouse Anti-ALDOA Recombinant Antibody (C10) in WB
Western blots with quantification of glucose transporter levels from hpBMEC and hiBMEC. Red = higher in hiBMEC, blue = higher in hpBMEC (significant with p < 0.05, LogFC > 0.6). ...View More
Weber, C. M., Moiz, B., Zic, S. M., Alpízar Vargas, V., Li, A., & Clyne, A. M. (2022). Induced pluripotent stem cell-derived cells model brain microvascular endothelial cell glucose metabolism. Fluids and Barriers of the CNS, 19(1), 1-15.
Mouse Anti-ALDOA Recombinant Antibody (C10) in WB
Levels of PPAR-γ were measured by Western blot analysis. Aldolase was used as a marker of cytosol, while lamin B was used as a nuclear marker. ...View More
Bae, S., Lim, J. W., & Kim, H. (2021). β-Carotene inhibits expression of matrix metalloproteinase-10 and invasion in Helicobacter pylori-infected gastric epithelial cells. Molecules, 26(6), 1567.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
C10
Antibody Isotype
IgG2b, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 319-336 of Aldolase A of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Aldolase, Fructose-Bisphosphate A
Introduction
This gene encodes a member of the class I fructose-bisphosphate aldolase protein family. The encoded protein is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Mutations in this gene have been associated with Glycogen Storage Disease XII, an autosomal recessive disorder associated with hemolytic anemia. Disruption of this gene also plays a role in the progression of multiple types of cancers. Related pseudogenes have been identified on chromosomes 3 and 10.
Entrez Gene ID
226
UniProt ID
P04075
Alternative Names
Aldolase, Fructose-Bisphosphate A; Aldolase A, Fructose-Bisphosphate; Lung Cancer Antigen NY-LU-1; Muscle-Type Aldolase; EC 4.1.2.13; ALDA;
Function
Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein (By similarity).
Biological Process
Actin filament organization Source: BHF-UCL
ATP biosynthetic process Source: BHF-UCL
Binding of sperm to zona pellucida Source: CAFA
Canonical glycolysis Source: Reactome
Fructose 1,6-bisphosphate metabolic process Source: BHF-UCL
Fructose metabolic process Source: BHF-UCL
Gluconeogenesis Source: Reactome
Glycolytic process Source: BHF-UCL
Muscle cell cellular homeostasis Source: BHF-UCL
Neutrophil degranulation Source: Reactome
Platelet degranulation Source: Reactome
Protein homotetramerization Source: UniProtKB
Regulation of cell shape Source: BHF-UCL
Striated muscle contraction Source: BHF-UCL
Cellular Location
I band; M line. In skeletal muscle, accumulates around the M line and within the I band, colocalizing with FBP2 on both sides of the Z line in the absence of Ca2+.
Involvement in disease
Glycogen storage disease 12 (GSD12): A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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