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Mouse Anti-FLG Recombinant Antibody (SPM181) (CBMAB-F1255-CQ)

This product is a mouse antibody that recognizes FLG. The antibody SPM181 can be used for immunoassay techniques such as: IHC-P, IF, FC.
See all FLG antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
SPM181
Antibody Isotype
IgG1, κ
Application
IHC-P, IF, FC

Basic Information

Immunogen
A recombinant human FLG protein
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1 mg/ml BSA
Preservative
0.05% sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Filaggrin
Introduction
The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.
Entrez Gene ID
2312
UniProt ID
P20930
Alternative Names
Filaggrin; Epidermal Filaggrin; ATOD2;
Function
Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
Biological Process
Establishment of skin barrier Source: UniProtKB
Keratinocyte differentiation Source: BHF-UCL
Multicellular organism development Source: UniProtKB
Peptide cross-linking Source: CAFA
Cellular Location
Cytoplasmic granule. In the stratum granulosum of the epidermis, localized within keratohyalin granules (PubMed:1429717). In granular keratinocytes and in lower corneocytes, colocalizes with calpain-1/CAPN1 (PubMed:21531719).
Involvement in disease
Ichthyosis vulgaris (VI):
The most common form of ichthyosis inherited as an autosomal dominant trait. It is characterized by palmar hyperlinearity, keratosis pilaris and a fine scale that is most prominent over the lower abdomen, arms, and legs. Ichthyosis vulgaris is characterized histologically by absent or reduced keratohyalin granules in the epidermis and mild hyperkeratosis. The disease can be associated with frequent asthma, eczema or hay fever.
Dermatitis atopic 2 (ATOD2):
Atopic dermatitis is a complex, inflammatory disease with multiple alleles at several loci thought to be involved in the pathogenesis. It commonly begins in infancy or early childhood and is characterized by a chronic relapsing form of skin inflammation, a disturbance of epidermal barrier function that culminates in dry skin, and IgE-mediated sensitization to food and environmental allergens. It is manifested by lichenification, excoriation, and crusting, mainly on the flexural surfaces of the elbow and knee.
PTM
Filaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 324 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. The N-terminal of the mature protein is heterogeneous, and is blocked by the formation of pyroglutamate.
Undergoes deimination of some arginine residues (citrullination).

Moosbrugger-Martinz, V., Leprince, C., Méchin, M. C., Simon, M., Blunder, S., Gruber, R., & Dubrac, S. (2022). Revisiting the Roles of Filaggrin in Atopic Dermatitis. International Journal of Molecular Sciences, 23(10), 5318.

Zhu, Y., Mitra, N., Feng, Y., Tishkoff, S., Hoffstad, O., & Margolis, D. (2021). Filaggrin variation differs for European-Americans and African-Americans. The Journal of investigative dermatology, 141(7), 1855.

Smieszek, S. P., Welsh, S., Xiao, C., Wang, J., Polymeropoulos, C., Birznieks, G., & Polymeropoulos, M. H. (2020). Correlation of age-of-onset of atopic dermatitis with filaggrin loss-of-function variant status. Scientific Reports, 10(1), 2721.

Combarros, D., Cadiergues, M. C., & Simon, M. (2020). Update on canine filaggrin: a review. Veterinary Quarterly, 40(1), 162-168.

Furue, M. (2020). Regulation of filaggrin, loricrin, and involucrin by IL-4, IL-13, IL-17A, IL-22, AHR, and NRF2: pathogenic implications in atopic dermatitis. International journal of molecular sciences, 21(15), 5382.

Thyssen, J. P., Jakasa, I., Riethmüller, C., Schön, M. P., Braun, A., Haftek, M., ... & Kezic, S. (2020). Filaggrin expression and processing deficiencies impair corneocyte surface texture and stiffness in mice. Journal of Investigative Dermatology, 140(3), 615-623.

Jurakic Toncic, R., Kezic, S., Jakasa, I., Ljubojevic Hadzavdic, S., Balic, A., Petkovic, M., ... & Marinovic, B. (2020). Filaggrin loss‐of‐function mutations and levels of filaggrin degradation products in adult patients with atopic dermatitis in Croatia. Journal of the European Academy of Dermatology and Venereology, 34(8), 1789-1794.

Čepelak, I., Dodig, S., & Pavić, I. (2019). Filaggrin and atopic march. Biochemia medica, 29(2), 214-227.

Margolis, D. J., Mitra, N., Gochnauer, H., Wubbenhorst, B., D’Andrea, K., Kraya, A., ... & Nathanson, K. L. (2018). Uncommon filaggrin variants are associated with persistent atopic dermatitis in African Americans. Journal of Investigative Dermatology, 138(7), 1501-1506.

Wong, X. C. C., Denil, S. L., Foo, J. N., Chen, H., Tay, A. S. L., Haines, R. L., ... & Common, J. E. (2018). Array-based sequencing of filaggrin gene for comprehensive detection of disease-associated variants. Journal of Allergy and Clinical Immunology, 141(2), 814-816.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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