Mouse Anti-FLG Recombinant Antibody (CBXF-2410) (CBMAB-F1601-CQ)

Name: Mouse Anti-FLG Recombinant Antibody (CBXF-2410)
SKU: CBMAB-F1601-CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-FLG Recombinant Antibody (CBXF-2410) (CBMAB-F1601-CQ) in IF

Effect of tempol administration on filaggrin, ZO-1 and occludin expression. Skin tissues from oxazolone-induced mice showed a lower expression of filaggrin (B, score E), ZO-1 (G, score J) and occludin (L, score O), compared to the respective sham groups (A, score E,F, score J,K, score O). Topical treatments with tempol 1% (C, score E,H, score J,M, score O) and tempol 2% (D, score E,I, score J,N, score O) notably increased the expression of filaggrin and TJs. White arrows indicate positive cells. In every experimental group, the number of mice was n = 10. The figures are shown at 40× magnification. Values are means ± SD. One-way ANOVA test. *** p < 0.001 vs. sham; ### p < 0.001 vs. oxazolone. ...View More

Ardizzone, A., Repici, A., Capra, A. P., De Gaetano, F., Bova, V., Casili, G., ... & Esposito, E. (2023). Efficacy of the Radical Scavenger, Tempol, to reduce inflammation and oxidative stress in a murine model of atopic dermatitis. Antioxidants, 12(6), 1278.

Mouse Anti-FLG Recombinant Antibody (CBXF-2410) (CBMAB-F1601-CQ) in IF

Effect of KYP-2047 administration on the expression level of ZO-1 and FLG by IF in intestine. (A) Control group intestinal cells' immunoreactivity for ZO-1. (B) IRI animals showed a significant loss of intestinal ZO-1. (C,D) An increase in ZO-1 cells' positivity was observed in IRI animals and subsequently treated with KYP-2047 (2.5 mg/kg and 5 mg/kg). (E) Immunofluorescence analysis of ZO-1. (F) The number of positive FLG cells was normal in the sham group. (G) A significant decrease in FLG cells' positivity was observed in IRI animals. (H,I) KYP-2047 treatments (2.5 mg/kg and 5 mg/kg) increased FLG cells' positivity in the intestine. (J) Immunofluorescence analysis of FLG. Data are representative of at least three independent experiments. *** p < 0.001 vs. Sham; ### p < 0.001 vs. IRI. ...View More

Filippone, A., Casili, G., Ardizzone, A., Lanza, M., Mannino, D., Paterniti, I., ... & Campolo, M. (2021). Inhibition of prolyl oligopeptidase prevents consequences of reperfusion following intestinal ischemia. Biomedicines, 9(10), 1354.

Mouse Anti-FLG Recombinant Antibody (CBXF-2410) (CBMAB-F1601-CQ) in WB

Reverse transcription-polymerase chain reaction and western blot analysis of PmRS on skin photoaging-related markers. ...View More

Son, H. U., Choi, H. J., Alam, M. B., Jeong, C. G., Lee, H. I., Kim, S. L., ... & Lee, S. H. (2021). Prunus mume seed exhibits inhibitory effect on skin senescence via SIRT1 and MMP-1 regulation. Oxidative Medicine and Cellular Longevity, 2021(1), 5528795.

Mouse Anti-FLG Recombinant Antibody (CBXF-2410) (CBMAB-F1601-CQ) in WB

Effects C-phycocyanin (C-PC) on (a) western blot band (b) involucrin, (c) filaggrin in ultraviolet B (UVB)-induced HaCaT cells, as assessed using Western blot analysis following treatment C-PC (20, 40, 80 μg/mL) ...View More

Jang, Y. A., & Kim, B. A. (2021). Protective effect of spirulina-derived C-phycocyanin against ultraviolet B-induced damage in HaCaT cells. Medicina, 57(3), 273.

Mouse Anti-FLG Recombinant Antibody (CBXF-2410) (CBMAB-F1601-CQ) in IF

IFA staining for filaggrin in the skin of the mice's backs. ...View More

Lee, W., Jeong, Y., Park, J. H., Lee, C. H., Yun, N., Lee, D. S., ... & Kim, S. (2019). Water-soluble extract from Actinidia arguta (Siebold & Zucc.) Planch. ex Miq. and Perilla frutescens (L.) Britton, ACTPER, ameliorates a dry skin-induced itch in a mice model and promotes filaggrin expression by activating the AhR signaling in HaCaT cells. Nutrients, 11(6), 1366.

Mouse Anti-FLG Recombinant Antibody (CBXF-2410) (CBMAB-F1601-CQ) in WB

Changes in the level of filaggrin protein. ...View More

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXF-2410

Application

WB, IP, IF, IHC-P

Immunogen

Human Filaggrin.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium azide

Concentration

0.1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Filaggrin

Introduction

The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.

Entrez Gene ID

2312

UniProt ID

P20930

Alternative Names

Filaggrin; Epidermal Filaggrin; ATOD2;

Function

Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.

Biological Process

Establishment of skin barrier Source: UniProtKB
Keratinocyte differentiation Source: BHF-UCL
Multicellular organism development Source: UniProtKB
Peptide cross-linking Source: CAFA

Cellular Location

Cytoplasmic granule. In the stratum granulosum of the epidermis, localized within keratohyalin granules (PubMed:1429717). In granular keratinocytes and in lower corneocytes, colocalizes with calpain-1/CAPN1 (PubMed:21531719).

Involvement in disease

Ichthyosis vulgaris (VI):
The most common form of ichthyosis inherited as an autosomal dominant trait. It is characterized by palmar hyperlinearity, keratosis pilaris and a fine scale that is most prominent over the lower abdomen, arms, and legs. Ichthyosis vulgaris is characterized histologically by absent or reduced keratohyalin granules in the epidermis and mild hyperkeratosis. The disease can be associated with frequent asthma, eczema or hay fever.
Dermatitis atopic 2 (ATOD2):
Atopic dermatitis is a complex, inflammatory disease with multiple alleles at several loci thought to be involved in the pathogenesis. It commonly begins in infancy or early childhood and is characterized by a chronic relapsing form of skin inflammation, a disturbance of epidermal barrier function that culminates in dry skin, and IgE-mediated sensitization to food and environmental allergens. It is manifested by lichenification, excoriation, and crusting, mainly on the flexural surfaces of the elbow and knee.

PTM

Filaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 324 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. The N-terminal of the mature protein is heterogeneous, and is blocked by the formation of pyroglutamate.
Undergoes deimination of some arginine residues (citrullination).

Moosbrugger-Martinz, V., Leprince, C., Méchin, M. C., Simon, M., Blunder, S., Gruber, R., & Dubrac, S. (2022). Revisiting the Roles of Filaggrin in Atopic Dermatitis. International Journal of Molecular Sciences, 23(10), 5318.

Zhu, Y., Mitra, N., Feng, Y., Tishkoff, S., Hoffstad, O., & Margolis, D. (2021). Filaggrin variation differs for European-Americans and African-Americans. The Journal of investigative dermatology, 141(7), 1855.

Smieszek, S. P., Welsh, S., Xiao, C., Wang, J., Polymeropoulos, C., Birznieks, G., & Polymeropoulos, M. H. (2020). Correlation of age-of-onset of atopic dermatitis with filaggrin loss-of-function variant status. Scientific Reports, 10(1), 2721.

Combarros, D., Cadiergues, M. C., & Simon, M. (2020). Update on canine filaggrin: a review. Veterinary Quarterly, 40(1), 162-168.

Furue, M. (2020). Regulation of filaggrin, loricrin, and involucrin by IL-4, IL-13, IL-17A, IL-22, AHR, and NRF2: pathogenic implications in atopic dermatitis. International journal of molecular sciences, 21(15), 5382.

Thyssen, J. P., Jakasa, I., Riethmüller, C., Schön, M. P., Braun, A., Haftek, M., ... & Kezic, S. (2020). Filaggrin expression and processing deficiencies impair corneocyte surface texture and stiffness in mice. Journal of Investigative Dermatology, 140(3), 615-623.

Jurakic Toncic, R., Kezic, S., Jakasa, I., Ljubojevic Hadzavdic, S., Balic, A., Petkovic, M., ... & Marinovic, B. (2020). Filaggrin loss‐of‐function mutations and levels of filaggrin degradation products in adult patients with atopic dermatitis in Croatia. Journal of the European Academy of Dermatology and Venereology, 34(8), 1789-1794.

Čepelak, I., Dodig, S., & Pavić, I. (2019). Filaggrin and atopic march. Biochemia medica, 29(2), 214-227.

Margolis, D. J., Mitra, N., Gochnauer, H., Wubbenhorst, B., D’Andrea, K., Kraya, A., ... & Nathanson, K. L. (2018). Uncommon filaggrin variants are associated with persistent atopic dermatitis in African Americans. Journal of Investigative Dermatology, 138(7), 1501-1506.

Wong, X. C. C., Denil, S. L., Foo, J. N., Chen, H., Tay, A. S. L., Haines, R. L., ... & Common, J. E. (2018). Array-based sequencing of filaggrin gene for comprehensive detection of disease-associated variants. Journal of Allergy and Clinical Immunology, 141(2), 814-816.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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