Mouse Anti-GPR182 Recombinant Antibody (CBFYH-0454) (CBMAB-H0603-FY)

This product is mouse antibody that recognizes GPR182. The antibody CBFYH-0454 can be used for immunoassay techniques such as: ELISA, WB.
See all GPR182 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBFYH-0454

Antibody Isotype

IgG2a, κ

Application

ELISA, WB

Basic Information

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

G protein-coupled receptor 182

Introduction

Adrenomedullin is a potent vasodilator peptide that exerts major effects on cardiovascular function. This gene encodes a seven-transmembrane protein that belongs to the family 1 of G-protein coupled receptors. Studies of the rat counterpart suggest that the encoded protein may function as a receptor for adrenomedullin.

Entrez Gene ID

11318

UniProt ID

O15218

Alternative Names

G Protein-Coupled Receptor 182; Adrenomedullin Receptor; ADMR; G-Protein Coupled Receptor 182; HrhAMR; Gamrh

Function

Orphan receptor.

Biological Process

Cell surface receptor signaling pathway Source: ProtInc

Cellular Location

Cell membrane

Topology

Extracellular: 1-57
Helical: 58-79
Cytoplasmic: 80-90
Helical: 91-113
Extracellular: 114-127
Helical: 128-149
Cytoplasmic: 150-170
Helical: 171-193
Extracellular: 194-217
Helical: 218-239
Cytoplasmic: 240-258
Helical: 259-280
Extracellular: 281-299
Helical: 300-320
Cytoplasmic: 321-404

References

Torphy, R. J., Sun, Y., Lin, R., Caffrey-Carr, A., Fujiwara, Y., Ho, F., ... & Zhu, Y. (2022). GPR182 limits antitumor immunity via chemokine scavenging in mouse melanoma models. Nature Communications, 13(1), 97.

Torphy, R. J. (2021). The Role of Endothelial G Protein-Coupled Receptor 182 (GPR182) in the Tumor Microenvironment (Doctoral dissertation, University of Colorado Denver, Anschutz Medical Campus).

Kwon, H. B., Mackie, D. I., Bonnavion, R., Mercier, A. L., Helker, C. S., Son, T., ... & Stainier, D. Y. (2020). The orphan G-protein coupled receptor 182 is a negative regulator of definitive hematopoiesis through leukotriene B4 signaling. ACS Pharmacology & Translational Science, 3(4), 676-689.

Schmid, C. D., Schledzewski, K., Mogler, C., Waldburger, N., Kalna, V., Marx, A., ... & Koch, P. S. (2018). GPR182 is a novel marker for sinusoidal endothelial differentiation with distinct GPCR signaling activity in vitro. Biochemical and biophysical research communications, 497(1), 32-38.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Datasheet
SDS
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