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Mouse Anti-GRK4 Recombinant Antibody (6D5) (CBMAB-G5000-LY)

This product is antibody recognizes GRK4. The antibody 6D5 immunoassay techniques such as: ELISA, WB.
See all GRK4 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
6D5
Antibody Isotype
IgG2b, κ
Application
ELISA, WB

Basic Information

Immunogen
Recombinant protein corresponding to aa36-145 from human GRK4 with GST tag. MW of the GST tag alone is 26kD
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
G protein-coupled receptor kinase 4
Introduction
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
Entrez Gene ID
2868
UniProt ID
P32298
Alternative Names
G Protein-Coupled Receptor Kinase 4; EC 2.7.11.16; GPRK2L; GPRK4; G Protein-Coupled Receptor Kinase 2-Like (Drosophila); G Protein-Coupled Receptor Kinase 2-Like;
Function
Specifically phosphorylates the activated forms of G protein-coupled receptors. GRK4-alpha can phosphorylate rhodopsin and its activity is inhibited by calmodulin; the other three isoforms do not phosphorylate rhodopsin and do not interact with calmodulin. GRK4-alpha and GRK4-gamma phosphorylate DRD3. Phosphorylates ADRB2.
Biological Process
G protein-coupled receptor internalization Source: Ensembl
Protein phosphorylation Source: GO_Central
Receptor internalization Source: UniProtKB
Regulation of G protein-coupled receptor signaling pathway Source: ProtInc
Regulation of rhodopsin mediated signaling pathway Source: Reactome
Regulation of signal transduction Source: GO_Central
Signal transduction Source: InterPro
Cellular Location
Cytoplasm; Cell cortex. Both at the cell surface and dispersed in the cytoplasm under basal conditions. Receptor stimulation results in the internalization of GRK4 to the perinuclear area, where colocalization with DRD3 is observed strongly at 5 and 15 minutes. DRD3 and GRK4 colocalize in lipid rafts of renal proximal tubule cells.
PTM
Palmitoylated.

Gerhards, J., Maerz, L. D., Matthees, E. S., Donow, C., Moepps, B., Premont, R. T., ... & Philipp, M. (2023). Kinase Activity Is Not Required for G Protein–Coupled Receptor Kinase 4 Restraining mTOR Signaling during Cilia and Kidney Development. The Journal of the American Society of Nephrology, 34(4), 590-606.

Yang, D., Tang, M., Zhang, M., Ren, H., Li, X., Zhang, Z., ... & Zeng, C. (2023). Down-regulation of G protein-coupled receptor kinase 4 protects against kidney ischemia-reperfusion injury. Kidney International.

Zhang, F., Lei, L., Huang, J., Wang, W., Su, Q., Yan, H., ... & Yang, J. (2022). G-protein-coupled receptor kinase 4 causes renal angiotensin II type 2 receptor dysfunction by increasing its phosphorylation. Clinical Science, 136(12), 989-1003.

Luo, Y., Wang, Z., Xiao, S., Li, R., & Jiang, X. (2022). G Protein-Coupled Receptor Kinase 4 Is a Novel Prognostic Factor in Hepatocellular Carcinoma. Disease Markers, 2022.

Li, L., Fu, W., Gong, X., Chen, Z., Tang, L., Yang, D., ... & Zeng, C. (2021). The role of G protein-coupled receptor kinase 4 in cardiomyocyte injury after myocardial infarction. European Heart Journal, 42(14), 1415-1430.

Yue, W., Tran, H. T., Wang, J. P., Schiermeyer, K., Gildea, J. J., Xu, P., & Felder, R. A. (2021). The hypertension related gene G-protein coupled receptor kinase 4 contributes to breast cancer proliferation. Breast Cancer: Basic and Clinical Research, 15, 11782234211015753.

Yaqub, D., Moore, S., Rozyyev, S., Konkalmatt, P., Asico, L., Hunt, J., ... & Jose, P. (2021). G Protein‐Coupled Receptor Kinase 4 (GRK4) Variant 65L‐Mediated Impairment of Dopamine D1 Receptor Function causes Hypertension. The FASEB Journal, 35.

Luo, Y., Huang, X., Yang, J., Huang, L., Li, R., Wu, Q., & Jiang, X. (2019). Proteomics analysis of G protein-coupled receptor kinase 4-inhibited cellular growth of HEK293 cells. Journal of proteomics, 207, 103445.

Lu, X., Ye, Z., Zheng, S., Ren, H., Zeng, J., Wang, X., ... & Zeng, C. (2018). Long‐Term Exposure of Fine Particulate Matter Causes Hypertension by Impaired Renal D1 Receptor–Mediated Sodium Excretion via Upregulation of G‐Protein–Coupled Receptor Kinase Type 4 Expression in Sprague‐Dawley Rats. Journal of the American Heart Association, 7(1), e007185.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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