GRK4

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. [provided by RefSeq]

Full Name

G protein-coupled receptor kinase 4

Function

Specifically phosphorylates the activated forms of G protein-coupled receptors. GRK4-alpha can phosphorylate rhodopsin and its activity is inhibited by calmodulin; the other three isoforms do not phosphorylate rhodopsin and do not interact with calmodulin. GRK4-alpha and GRK4-gamma phosphorylate DRD3. Phosphorylates ADRB2.

Biological Process

G protein-coupled receptor internalization Source: Ensembl
Protein phosphorylation Source: GO_Central
Receptor internalization Source: UniProtKB
Regulation of G protein-coupled receptor signaling pathway Source: ProtInc
Regulation of rhodopsin mediated signaling pathway Source: Reactome
Regulation of signal transduction Source: GO_Central
Signal transduction Source: InterPro

Cellular Location

Cytoplasm; Cell cortex. Both at the cell surface and dispersed in the cytoplasm under basal conditions. Receptor stimulation results in the internalization of GRK4 to the perinuclear area, where colocalization with DRD3 is observed strongly at 5 and 15 minutes. DRD3 and GRK4 colocalize in lipid rafts of renal proximal tubule cells.

PTM

Palmitoylated.

Anti-GRK4 antibodies

Mouse Anti-GRK4 Recombinant Antibody (6D5) (CBMAB-G5000-LY)

Datasheet

SDS

Target: GRK4

Host: Mouse

Antibody Isotype: IgG2b, κ

Specificity: Human

Clone: 6D5

Application*: E, WB

Mouse Anti-GRK4 Recombinant Antibody (CBLG1-2967) (CBMAB-G5003-LY)

Datasheet

SDS

Target: GRK4

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: CBLG1-2967

Application*: WB

Mouse Anti-GRK4 Recombinant Antibody (CBLG1-2968) (CBMAB-G5004-LY)

Datasheet

SDS

Target: GRK4

Host: Mouse

Antibody Isotype: IgG2a

Specificity: Human

Clone: CBLG1-2968

Application*: WB

Mouse Anti-GRK4 Recombinant Antibody (2C5) (CBMAB-G4997-LY)

Datasheet

SDS

Target: GRK4

Host: Mouse

Antibody Isotype: IgG1, κ

Specificity: Human

Clone: 2C5

Application*: E, WB

Mouse Anti-GRK4 Recombinant Antibody (CBLG1-1784) (CBMAB-G5005-LY)

Datasheet

SDS

Target: GRK4

Host: Mouse

Antibody Isotype: IgG2b, κ

Specificity: Human

Clone: CBLG1-1784

Application*: E, WB

Mouse Anti-GRK4 (AA 36-145) Recombinant Antibody (CBFYH-0486) (CBMAB-H1356-FY)

Datasheet

SDS

Target: GRK4

Host: Mouse

Antibody Isotype: IgG2b, κ

Specificity: Human

Clone: CBFYH-0486

Application*: E, WB

For Research Use Only. Not For Clinical Use.

(P): Predicted

* Abbreviations

IFImmunofluorescence

IHImmunohistochemistry

IPImmunoprecipitation

WBWestern Blot

EELISA

MMicroarray

CIChromatin Immunoprecipitation

FFlow Cytometry

FNFunction Assay

IDImmunodiffusion

RRadioimmunoassay

TCTissue Culture

GSGel Supershift

NNeutralization

BBlocking

AActivation

IInhibition

DDepletion

ESELISpot

DBDot Blot

MCMass Cytometry/CyTOF

CTCytotoxicity

SStimulation

AGAgonist

APApoptosis

IMImmunomicroscopy

BABioassay

CSCostimulation

EMElectron Microscopy

IEImmunoelectrophoresis

PAPeptide Array

ICImmunocytochemistry

PEPeptide ELISA

MDMeDIP

SHIn situ hybridization

IAEnzyme Immunoassay

SEsandwich ELISA

PLProximity Ligation Assay

ECELISA(Cap)

EDELISA(Det)

BIBioimaging

IOImmunoassay

LFLateral Flow Immunoassay

LALuminex Assay

CImmunohistochemistry-Frozen Sections

PImmunohistologyp-Paraffin Sections

ISIntracellular Staining for Flow Cytometry

MSElectrophoretic Mobility Shift Assay

RIRNA Binding Protein Immunoprecipitation (RIP)