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Mouse Anti-AFM Recombinant Antibody (V2-634159) (CBMAB-AP185LY)


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Published Data
Mouse Anti-AFM Recombinant Antibody (790) (CBMAB-AP185LY) in WB
Overexcretion of alpha-1-antitrypsin (A1AT) and afamin (AFM) in urine from patients with PMN. a Representative images of Western blot. Bar chats of relative fold-change of A1AT (b) and AFM (c) of internal validation, and A1AT (d) and AFM (e) of external validation. Asterisk denotes P < 0.05 using student t test. The bars represent the means ± standard deviations. ...View More
Pang, L., Li, Q., Li, Y., Liu, Y., Duan, N., & Li, H. (2018). Urine proteomics of primary membranous nephropathy using nanoscale liquid chromatography tandem mass spectrometry analysis. Clinical Proteomics, 15, 1-15.

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-634159
Antibody Isotype
IgG1
Application
ELISA, IF, IP, WB

Basic Information

Immunogen
Fusion protein of AFM
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
IF(ICC)1:50-1:500
ELISA1:100-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Afamin
Introduction
This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human173
Mouse280662
UniProt ID
HumanP43652
MouseO89020
Alternative Names
Afamin; Alpha-Albumin; Alpha-Alb; ALB2; ALBA; ALF;
Function
Functions as carrier for hydrophobic molecules in body fluids (Probable). Essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B. Binds vitamin E. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the transport of vitamin E across the blood-brain barrier.
Biological Process
Protein stabilization
Protein transport within extracellular region
Vitamin transport
Cellular Location
Secreted
PTM
N-glycosylated; more than 90% of the glycans are sialylated.
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For research use only. Not intended for any clinical use.

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