Mouse Anti-AFM Recombinant Antibody (V2-180060) (CBMAB-A1470-YC)

Name: Mouse Anti-AFM Recombinant Antibody (V2-180060)
SKU: CBMAB-A1470-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AFM Recombinant Antibody (CBYC-A400) (CBMAB-A1470-YC) in ELISA

ELISA analysis of sera from the same 11 GC patients and 13 healthy controls. P-values were calculated using the Mann-Whitney U test (only significant results shown). Bar represents the group mean. ...View More

Penno, M. A., Klingler-Hoffmann, M., Brazzatti, J. A., Boussioutas, A., Putoczki, T., Ernst, M., & Hoffmann, P. (2012). 2D-DIGE analysis of sera from transgenic mouse models reveals novel candidate protein biomarkers for human gastric cancer. Journal of proteomics, 77, 40-58.

Mouse Anti-AFM Recombinant Antibody (CBYC-A400) (CBMAB-A1470-YC) in ELISA

Box-and-whisker plots showing afamin plasma concentrations in 528 healthy blood donors across different age groups. Median afamin plasma concentrations according to age group: 18-24 years (n = 94), 71 mg/L (range 33-106 mg/L); 25-34 years (n =131), 66 mg/L (range 40-98 mg/L); 35-44 years (n = 128), 66 mg/L (range 43-109 mg/L); 45-54 years (n = 127), 70 mg/L (range 33-113 mg/L); and 55-64 years (n = 48), 68 mg/L (range 38-102 mg/L). No significant differences in afamin plasma concentrations were observed across these age groups (Kruskal-Wallis test, p = 0.104). ...View More

Dieplinger, H., & Dieplinger, B. (2015). Afamin-A pleiotropic glycoprotein involved in various disease states. Clinica Chimica Acta, 446, 105-110.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-180060

Application

WB, ICC, IHC-P, ELISA

Immunogen

Afamin

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.4, 0.02% sodium azide, 50% glycerol

Buffer

PBS, pH7.4, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Afamin

Introduction

AFM is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. AFM is r

Entrez Gene ID

173

UniProt ID

P43652

Alternative Names

ALB2; ALBA; ALF

Function

Functions as carrier for hydrophobic molecules in body fluids (Probable). Essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B. Binds vitamin E. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the transport of vitamin E across the blood-brain barrier.

Biological Process

Protein stabilization
Protein transport within extracellular region
Vitamin transport

Cellular Location

Secreted

PTM

N-glycosylated; more than 90% of the glycans are sialylated.

Nuñez-Calonge, R., Cortes, S., Peregrín, P. C., Gonzalez, L. M. G., & Kireev, R. (2021). Seminal Plasma and Serum Afamin Levels Are Associated with Infertility in Men with Oligoasthenoteratozoospermia. Reproductive Sciences, 28(5), 1498-1506.

Wang, W. K., Tsai, C. H., Liu, Y. W., Lai, C. C., Huang, C. C., & Sheen-Chen, S. M. (2020). Afamin expression in breast cancer. Asian journal of surgery, 43(7), 750-754.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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