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Mouse Anti-AFM Recombinant Antibody (V2-180060) (CBMAB-A1470-YC)

Provided herein is a Mouse monoclonal antibody against Human Afamin. The antibody can be used for immunoassay techniques, such as WB, ICC, IHC-P, IHC-Fr, ELISA.
See all AFM antibodies
Published Data
Mouse Anti-AFM Recombinant Antibody (CBYC-A400) (CBMAB-A1470-YC) in ELISA
ELISA analysis of sera from the same 11 GC patients and 13 healthy controls. P-values were calculated using the Mann-Whitney U test (only significant results shown). Bar represents the group mean. ...View More
Penno, M. A., Klingler-Hoffmann, M., Brazzatti, J. A., Boussioutas, A., Putoczki, T., Ernst, M., & Hoffmann, P. (2012). 2D-DIGE analysis of sera from transgenic mouse models reveals novel candidate protein biomarkers for human gastric cancer. Journal of proteomics, 77, 40-58.
Mouse Anti-AFM Recombinant Antibody (CBYC-A400) (CBMAB-A1470-YC) in ELISA
Box-and-whisker plots showing afamin plasma concentrations in 528 healthy blood donors across different age groups. Median afamin plasma concentrations according to age group: 18-24 years (n = 94), 71 mg/L (range 33-106 mg/L); 25-34 years (n =131), 66 mg/L (range 40-98 mg/L); 35-44 years (n = 128), 66 mg/L (range 43-109 mg/L); 45-54 years (n = 127), 70 mg/L (range 33-113 mg/L); and 55-64 years (n = 48), 68 mg/L (range 38-102 mg/L). No significant differences in afamin plasma concentrations were observed across these age groups (Kruskal-Wallis test, p = 0.104). ...View More
Dieplinger, H., & Dieplinger, B. (2015). Afamin-A pleiotropic glycoprotein involved in various disease states. Clinica Chimica Acta, 446, 105-110.

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-180060
Antibody Isotype
IgG
Application
WB, ICC, IHC-P, ELISA

Basic Information

Immunogen
Afamin
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
PBS, pH 7.4, 0.02% sodium azide, 50% glycerol
Buffer
PBS, pH7.4, 50% glycerol
Preservative
0.02% sodium azide
Concentration
1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Afamin
Introduction
AFM is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. AFM is r
Entrez Gene ID
173
UniProt ID
P43652
Alternative Names
ALB2; ALBA; ALF
Function
Functions as carrier for hydrophobic molecules in body fluids (Probable). Essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B. Binds vitamin E. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the transport of vitamin E across the blood-brain barrier.
Biological Process
Protein stabilization
Protein transport within extracellular region
Vitamin transport
Cellular Location
Secreted
PTM
N-glycosylated; more than 90% of the glycans are sialylated.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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