Human AFM ELISA Kit (V2LY-0626-LY3832)

Name: Human AFM ELISA Kit
SKU: V2LY-0626-LY3832
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

2.38 ng/mL

Detection Range

5-600 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

69.1 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Afamin

Function

Functions as carrier for hydrophobic molecules in body fluids (Probable). Essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B. Binds vitamin E. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the transport of vitamin E across the blood-brain barrier.

Biological Process

Protein stabilization
Protein transport within extracellular region
Vitamin transport

Cellular Location

Secreted

PTM

N-glycosylated; more than 90% of the glycans are sialylated.

Nuñez-Calonge, R., Cortes, S., Peregrín, P. C., Gonzalez, L. M. G., & Kireev, R. (2021). Seminal Plasma and Serum Afamin Levels Are Associated with Infertility in Men with Oligoasthenoteratozoospermia. Reproductive Sciences, 28(5), 1498-1506.

Wang, W. K., Tsai, C. H., Liu, Y. W., Lai, C. C., Huang, C. C., & Sheen-Chen, S. M. (2020). Afamin expression in breast cancer. Asian journal of surgery, 43(7), 750-754.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant AFM protein, His Tag (CAT#: V2LY-0526-LY1956)

Rat Afm ELISA Kit (CAT#: V2LY-0626-LY1528)

Related Products

Mouse Anti-AFM Recombinant Antibody (V2-180059) (CAT#: CBMAB-A1469-YC)

Rat Afm ELISA Kit (CAT#: V2LY-0626-LY1528)

Mouse Anti-AFM Recombinant Antibody (V2-634159) (CAT#: CBMAB-AP185LY)

Mouse Anti-AFM Recombinant Antibody (V2-180057) (CAT#: CBMAB-A1467-YC)

Human Recombinant AFM protein, His Tag (CAT#: V2LY-0526-LY1956)

Mouse Anti-AFM Recombinant Antibody (V2-180060) (CAT#: CBMAB-A1470-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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