Mouse Anti-AGK Recombinant Antibody (V2-258056) (CBMAB-M0989-FY)

Name: Mouse Anti-AGK Recombinant Antibody (V2-258056)
SKU: CBMAB-M0989-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AGK Recombinant Antibody (CBFYM-0842) (CBMAB-M0989-FY) in Immunoblot

Immunoblot analysis of the indicated proteins in whole-cell extracts (WL), nuclear (NF), cytoplasmic (CF), total membrane (MF), and plasma membrane (PF) fractions of wild-type CD4+ T cells (CD4) and CD8+ T cells (CD8). ...View More

Hu, Z., Qu, G., Yu, X., Jiang, H., Teng, X. L., Ding, L., ... & Zou, Q. (2019). Acylglycerol kinase maintains metabolic state and immune responses of CD8+ T cells. Cell Metabolism, 30(2), 290-302.

Mouse Anti-AGK Recombinant Antibody (CBFYM-0842) (CBMAB-M0989-FY) in FC

Flow cytometric analysis of p-S6 expression in Agk+/+Cd4-Cre, Agkfl/flCd4-Cre, Ptenfl/flCd4-Cre and Agkfl/flPtenfl/flCd4-Cre naive CD8+ T cells stimulated for 2 h with antibodies against CD3 and CD28. The numbers above the graphs indicate mean fluorescence intensity (MFI). ...View More

Hu, Z., Qu, G., Yu, X., Jiang, H., Teng, X. L., Ding, L., ... & Zou, Q. (2019). Acylglycerol kinase maintains metabolic state and immune responses of CD8+ T cells. Cell Metabolism, 30(2), 290-302.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-258056

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 377-398 near the C-terminus of MULK of human origin.

Host Species

Mouse

Specificity

Mouse, Rat, Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
IF(ICC)	1:50-1:500
ELISA	1:100-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

acylglycerol kinase

Introduction

The protein encoded by this gene is a mitochondrial membrane protein involved in lipid and glycerolipid metabolism. The encoded protein is a lipid kinase that catalyzes the formation of phosphatidic and lysophosphatidic acids. Defects in this gene have been associated with mitochondrial DNA depletion syndrome 10.

Entrez Gene ID

Human	55750
Mouse	69923
Rat	502749

UniProt ID

Human	Q53H12
Mouse	Q9ESW4
Rat	D3Z9L0

Alternative Names

Acylglycerol Kinase; Multiple Substrate Lipid Kinase; Multi-Substrate Lipid Kinase; HsMuLK; MULK; HAGK; Acylglycerol Kinase, Mitochondrial

Function

Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Phosphorylates ceramide (By similarity). Phosphorylates 1,2-dioleoylglycerol more rapidly than 2,3-dioleoylglycerol (By similarity). Independently of its lipid kinase activity, acts as a component of the TIM22 complex. The TIM22 complex mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane by forming a twin-pore translocase that uses the membrane potential as the external driving force. In the TIM22 complex, required for the import of a subset of metabolite carriers into mitochondria, such as ANT1/SLC25A4 and SLC25A24, while it is not required for the import of TIMM23. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth.

Biological Process

Ceramide biosynthetic process
Glycerophospholipid biosynthetic process
Phosphorylation
Protein insertion into mitochondrial inner membrane
Sphingolipid metabolic process
Sphingosine biosynthetic process

Cellular Location

Mitochondrion inner membrane; Mitochondrion intermembrane space. Localizes in the mitochondrion intermembrane space, where it associates with the inner membrane. It is unclear whether the N-terminal hydrophobic region forms a transmembrane region or associates with the membrane without crossing it.

Involvement in disease

Mitochondrial DNA depletion syndrome 10 (MTDPS10): The TIM22 complex and import of proteins into mitochondrion are affected in patients suffering of MTDPS10. An autosomal recessive mitochondrial disorder characterized by congenital cataracts, hypertrophic cardiomyopathy, skeletal myopathy, exercise intolerance, and lactic acidosis. Mental development is normal, but affected individuals may die early from cardiomyopathy.
Cataract 38 (CTRCT38): An opacification of the crystalline lens of the eye becoming evident at birth. It frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function.

Sun, F., Ma, Y., Jie, Q., & Li, Q. (2021). P–422 Acylglycerol Kinase is a new novel marker in preeclampsia. Human Reproduction, 36(Supplement_1), deab130-421.

Zhao, C., Chen, H. Y., Zhao, F., Feng, H. J., & Su, J. P. (2021). Acylglycerol kinase promotes paclitaxel resistance in nasopharyngeal carcinoma cells by regulating FOXM1 via the JAK2/STAT3 pathway. Cytokine, 155595.

Zhu, Q., Zhong, A. L., Hu, H., Zhao, J. J., Weng, D. S., Tang, Y., ... & Xia, J. C. (2020). Acylglycerol kinase promotes tumour growth and metastasis via activating the PI3K/AKT/GSK3β signalling pathway in renal cell carcinoma. Journal of hematology & oncology, 13(1), 1-16.

Huang, S., Cao, Y., Guo, H., Yao, Y., Li, L., Chen, J., ... & Xiong, J. (2020). Up‐regulated acylglycerol kinase (AGK) expression associates with gastric cancer progression through the formation of a novel YAP1‐AGK–positive loop. Journal of cellular and molecular medicine, 24(19), 11133-11145.

Zhao, Q., Sun, P., Qin, S., & Liu, J. (2020). Acylglycerol kinase promotes the stemness of nasopharyngeal carcinoma cells by promoting β‐catenin translocation to the nucleus through activating PI3K/Akt pathway. Environmental Toxicology, 35(12), 1299-1307.

Hu, Z., Qu, G., Yu, X., Jiang, H., Teng, X. L., Ding, L., ... & Zou, Q. (2019). Acylglycerol kinase maintains metabolic state and immune responses of CD8+ T cells. Cell metabolism, 30(2), 290-302.

Vukotic, M., Nolte, H., König, T., Saita, S., Ananjew, M., Krüger, M., ... & Langer, T. (2017). Acylglycerol kinase mutated in Sengers syndrome is a subunit of the TIM22 protein translocase in mitochondria. Molecular cell, 67(3), 471-483.

Kang, Y., Stroud, D. A., Baker, M. J., De Souza, D. P., Frazier, A. E., Liem, M., ... & Stojanovski, D. (2017). Sengers syndrome-associated mitochondrial acylglycerol kinase is a subunit of the human TIM22 protein import complex. Molecular cell, 67(3), 457-470.

Mårtensson, C. U., & Becker, T. (2017). Acylglycerol kinase: mitochondrial protein transport meets lipid biosynthesis. Trends in cell biology, 27(10), 700-702.

Houten, S. M. (2017). Protein moonlighting in inborn errors of metabolism: The case of the mitochondrial acylglycerol kinase. Journal of inherited metabolic disease, 40(6), 755.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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