What applications is the Mouse Anti-Acetyl SMC3 (K105/K106) Recombinant Antibody validated for?

Based on common validation practices and my experience, this antibody (CAT: CBMAB-AP052LY) is typically validated and performs exceptionally well in Western Blotting (WB), Immunofluorescence (IF), and Chromatin Immunoprecipitation (ChIP). Creative Biolabs often provides detailed data sheets for each product that list all validated applications and recommended starting dilutions. If you're considering an application not listed, it's always a good idea to check their website or inquire about trial sizes.

How should I determine the optimal dilution for my specific experiment?

While Creative Biolabs provides recommended dilution ranges (e.g., 1:500-1:2000 for Western Blotting), the optimal dilution can vary depending on your specific experimental setup, sample type, and detection system sensitivity. It's always best practice to perform a titration experiment to find the ideal dilution for your particular application. Start with the recommended range and test a few dilutions above and below to achieve the best signal-to-noise ratio.

Mouse Anti-Acetyl SMC3 (K105/K106) Recombinant Antibody (V2-634053) (CBMAB-AP052LY)

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Published Data

Mouse Anti-Acetyl SMC3 (K105/K106) Recombinant Antibody (21A7) in WB

Western blot analysis following co-immunoprecipitation of IgG (negative control), SMC3, and NIPBL in WT siGLO and Pds5a−/− siPds5b nuclear lysates, under high stringency conditions. Black triangle indicates the NIPBL-specific band (top), as determined via NIPBL knockdown (see Additional file 2: Figure S6D). ...View More

Arruda, N. L., Bryan, A. F., & Dowen, J. M. (2022). PDS5A and PDS5B differentially affect gene expression without altering cohesin localization across the genome. Epigenetics & Chromatin, 15(1), 1-20.

Mouse Anti-Acetyl SMC3 (K105/K106) Recombinant Antibody (21A7) in Immunoblot

ACY-241 and PCI-34051 treatment synergistically enhances p53 stability in p53 wild-type ovarian cancer cells. (A) Immunoblotting of acetyl-p53. Cells were treated with indicated doses of PCI-34051 for 24 h. (B,C) Immunoblotting of acetyl-p53 and p21. Cells were treated with 3 µM ACY-241 and 20 µM PCI-34051 alone or in combination for 24 h. Protein expression levels were semi-quantified relative to the loading control, α-tubulin. Relative protein levels of acetyl-p53 were semi-quantified relative to total p53 levels and relative protein levels of acetyl SMC3 were semi-quantified relative to total SMC3 levels. Data are presented as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, or *** p < 0.001 vs. DMSO control; ## p < 0.01, or ### p < 0.001 vs. ACY-241-treated group; $ p < 0.05, $$ p < 0.01, or $$$ p < 0.001 vs. PCI-34051-treated group. ...View More

Kim, J. Y., Han, S. Y., Yoo, J., Kim, G. W., Jeon, Y. H., Lee, S. W., ... & Kwon, S. H. (2022). HDAC8-Selective Inhibition by PCI-34051 Enhances the Anticancer Effects of ACY-241 in Ovarian Cancer Cells. International Journal of Molecular Sciences, 23(15), 8645.

Mouse Anti-Acetyl SMC3 (K105/K106) Recombinant Antibody (21A7) in Immunoblot

Quantitative immunoblots of whole protein from testis tissues. RIPA fractions from 5-month-old WT and matched CLPP-null testis were analyzed for acetyl-SMC3. ...View More

Key, J., Gispert, S., Koornneef, L., Sleddens-Linkels, E., Kohli, A., Torres-Odio, S., ... & Auburger, G. (2022). CLPP Depletion Causes Diplotene Arrest; Underlying Testis Mitochondrial Dysfunction Occurs with Accumulation of Perrault Proteins ERAL1, PEO1, and HARS2. Cells, 12(1), 52.

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

V2-634053

Application

WB, ELISA

Immunogen

SMC3 containing acetylated Lys105 and Lys106

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Tris-Glycine, pH 7.4

Preservative

0.05% Sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Structural Maintenance Of Chromosomes 3

Introduction

This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein. [provided by RefSeq, Jul 2008]

Entrez Gene ID

100145671

UniProt ID

O93309

Alternative Names

Structural Maintenance Of Chromosomes 3; Basement Membrane-Associated Chondroitin Proteoglycan; Chondroitin Sulfate Proteoglycan 6 (Bamacan); Chromosome-Associated Polypeptide; SMC Protein 3; SMC3L1; CSPG6; HCAP; BAM;

Function

Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.

Biological Process

Cell division
DNA repair
Meiotic cell cycle
Mitotic cell cycle
Mitotic spindle assembly
Regulation of DNA replication
Sister chromatid cohesion
Stem cell population maintenance

Cellular Location

Nucleus; Chromosome; Centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. The phosphorylated form at Ser-1083 is preferentially associated with unsynapsed chromosomal regions (By similarity).

Involvement in disease

A form of Cornelia de Lange syndrome, a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. Characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation, hirsutism, gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies. Cornelia de Lange syndrome type 3 is a mild form with absence of major structural anomalies. The phenotype in some instances approaches that of apparently non-syndromic mental retardation.

PTM

Ubiquitinated by the DCX(DCAF15) complex, leading to its degradation.
Phosphorylated at Ser-1083 in a SPO11-dependent manner.
Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication. Deacetylation by HDAC8, regulates release of the cohesin complex from chromatin.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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