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Mouse Anti-HIC1 Recombinant Antibody (4E11) (CBMAB-A3892-LY)

The product is antibody recognizes HIC1. The antibody 4E11 immunoassay techniques such as: WB, ELISA.
See all HIC1 antibodies
Published Data
Fig.1 Targeted HIC1 and RassF1A methylation reduced expression, reversed by 5-aza-20-deoxycytidine (5-Aza) treatment.
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Fig.2 Immunostaining of MSCs, me_HR-transformed MSCs, and 5-Aza-treated me_HR-transformed.
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Summary

Host Animal
Mouse
Specificity
Human, Rat
Clone
4E11
Antibody Isotype
IgG2b, κ
Application
WB, ELISA

Basic Information

Immunogen
HIC1 (NP_006488, 396 a.a. ~ 453 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
hypermethylated in cancer 1
Entrez Gene ID
Human3090
Rat303310
UniProt ID
HumanQ14526
RatD4A974
Alternative Names
ZBTB29; hic-1
Function
Transcriptional repressor (PubMed:12052894, PubMed:15231840).

Recognizes and binds to the consensus sequence '5-[CG]NG[CG]GGGCA[CA]CC-3' (PubMed:15231840).

May act as a tumor suppressor (PubMed:20154726).

Involved in development of head, face, limbs and ventral body wall (By similarity).

Involved in down-regulation of SIRT1 and thereby is involved in regulation of p53/TP53-dependent apoptotic DNA-damage responses (PubMed:16269335).

The specific target gene promoter association seems to be depend on corepressors, such as CTBP1 or CTBP2 and MTA1 (PubMed:12052894, PubMed:20547755).

In cooperation with MTA1 (indicative for an association with the NuRD complex) represses transcription from CCND1/cyclin-D1 and CDKN1C/p57Kip2 specifically in quiescent cells (PubMed:20547755).

Involved in regulation of the Wnt signaling pathway probably by association with TCF7L2 and preventing TCF7L2 and CTNNB1 association with promoters of TCF-responsive genes (PubMed:16724116).

Seems to repress transcription from E2F1 and ATOH1 which involves ARID1A, indicative for the participation of a distinct SWI/SNF-type chromatin-remodeling complex (PubMed:18347096, PubMed:19486893).

Probably represses transcription of ACKR3, FGFBP1 and EFNA1 (PubMed:16690027, PubMed:19525223, PubMed:20154726).
Biological Process
Intrinsic apoptotic signaling pathway in response to DNA damage Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Negative regulation of Wnt signaling pathway Source: UniProtKB
Positive regulation of DNA damage response, signal transduction by p53 class mediator Source: UniProtKB
Regulation of transcription, DNA-templated Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Wnt signaling pathway Source: UniProtKB-KW
Cellular Location
Nucleus
PTM
Acetylated on several residues, including Lys-333. Lys-333 is deacetylated by SIRT1.
Sumoylated on Lys-333 by a PIAS family member, which enhances interaction with MTA1, positively regulates transcriptional repression activity and is enhanced by HDAC4.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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