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Mouse Anti-HIC1 (AA 396-453) Recombinant Antibody (CBFYH-1074) (CBMAB-H2017-FY)

This product is mouse antibody that recognizes HIC1. The antibody CBFYH-1074 can be used for immunoassay techniques such as: ELISA, IF, WB.
See all HIC1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYH-1074
Antibody Isotype
IgG2a, κ
Application
ELISA, IF, WB

Basic Information

Immunogen
Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Immunogen sequence: YKSSSEETGS SEDPSPPGGH LEGYPCPHLA YGEPESFGDN LYVCIPCGKG FPSSEQLN
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 396-453

Target

Full Name
hypermethylated in cancer 1
Introduction
This gene functions as a growth regulatory and tumor repressor gene. Hypermethylation or deletion of the region of this gene have been associated with tumors and the contiguous-gene syndrome, Miller-Dieker syndrome. Alternative splicing of this gene results in multiple transcript variants.
Entrez Gene ID
3090
UniProt ID
Q14526
Alternative Names
HIC ZBTB Transcriptional Repressor 1; Zinc Finger And BTB Domain-Containing Protein 29; Hypermethylated In Cancer 1; ZBTB29; Hic-1; Hypermethylated In Cancer 1 Protein; ZNF901
Function
Transcriptional repressor (PubMed:12052894, PubMed:15231840).

Recognizes and binds to the consensus sequence '5-[CG]NG[CG]GGGCA[CA]CC-3' (PubMed:15231840).

May act as a tumor suppressor (PubMed:20154726).

Involved in development of head, face, limbs and ventral body wall (By similarity).

Involved in down-regulation of SIRT1 and thereby is involved in regulation of p53/TP53-dependent apoptotic DNA-damage responses (PubMed:16269335).

The specific target gene promoter association seems to be depend on corepressors, such as CTBP1 or CTBP2 and MTA1 (PubMed:12052894, PubMed:20547755).

In cooperation with MTA1 (indicative for an association with the NuRD complex) represses transcription from CCND1/cyclin-D1 and CDKN1C/p57Kip2 specifically in quiescent cells (PubMed:20547755).

Involved in regulation of the Wnt signaling pathway probably by association with TCF7L2 and preventing TCF7L2 and CTNNB1 association with promoters of TCF-responsive genes (PubMed:16724116).

Seems to repress transcription from E2F1 and ATOH1 which involves ARID1A, indicative for the participation of a distinct SWI/SNF-type chromatin-remodeling complex (PubMed:18347096, PubMed:19486893).

Probably represses transcription of ACKR3, FGFBP1 and EFNA1 (PubMed:16690027, PubMed:19525223, PubMed:20154726).
Biological Process
Intrinsic apoptotic signaling pathway in response to DNA damage Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Negative regulation of Wnt signaling pathway Source: UniProtKB
Positive regulation of DNA damage response, signal transduction by p53 class mediator Source: UniProtKB
Regulation of transcription, DNA-templated Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Wnt signaling pathway Source: UniProtKB-KW
Cellular Location
Nucleus
PTM
Acetylated on several residues, including Lys-333. Lys-333 is deacetylated by SIRT1.
Sumoylated on Lys-333 by a PIAS family member, which enhances interaction with MTA1, positively regulates transcriptional repression activity and is enhanced by HDAC4.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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