Mouse Anti-HVCN1 Recombinant Antibody (A344) (CBMAB-AP10807LY)

Name: Mouse Anti-HVCN1 Recombinant Antibody (A344)
SKU: CBMAB-AP10807LY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

A344

Antibody Isotype

IgG1

Application

ELISA, IHC, WB

Basic Information

Immunogen

Fusion protein of HVCN1

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

Affinity purity

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Hydrogen Voltage Gated Channel 1

Introduction

This gene encodes a voltage-gated protein channel protein expressed more highly in certain cells of the immune system. Phagocytic cells produce superoxide anions which require this channel protein, and in B cells this same process facilitates antibody production. This same channel protein, however, can also regulate functions in other cells including spermatozoa. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Entrez Gene ID

84329

UniProt ID

Q96D96

Alternative Names

Hydrogen Voltage Gated Channel 1; Voltage Sensor Domain-Only Protein; Hydrogen Voltage-Gated Channel 1; VSOP; HV1; Voltage-Gated Hydrogen Channel 1;

Function

Mediates the voltage-dependent proton permeability of excitable membranes. Forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis.

Biological Process

Cell redox homeostasis Source: Reactome
Cellular response to pH Source: UniProtKB
Cellular response to zinc ion Source: UniProtKB
Positive regulation of superoxide anion generation Source: Ensembl
Proton transmembrane transport Source: UniProtKB
Regulation of intracellular pH Source: Ensembl
Regulation of ion transmembrane transport Source: UniProtKB-KW
Response to pH Source: HGNC-UCL
Response to zinc ion Source: HGNC-UCL

Cellular Location

Cell membrane; Membrane. Detected mainly at intracellular membranes upon overexpression in HeLa cells (PuMed:20147290), but not in other cell types.

Topology

Cytoplasmic: 1-100
Helical: 101-121
Extracellular: 122-138
Helical: 139-161
Cytoplasmic: 162-169
Helical: 170-190
Extracellular: 191-197
Helical: 198-218
Cytoplasmic: 219-273

PTM

Phosphorylation may enhance channel gating.

More Infomation

References

Ma, J., Gao, X., Li, Y., DeCoursey, T. E., Shull, G. E., & Wang, H. S. (2022). The HVCN1 voltage‐gated proton channel contributes to pH regulation in canine ventricular myocytes. The Journal of Physiology, 600(9), 2089-2103.

Wu, X., Li, Y., Maienschein-Cline, M., Feferman, L., Wu, L., & Hong, L. (2022). RNA-seq analyses reveal roles of the HVCN1 proton channel in cardiac pH homeostasis. Frontiers in Cell and Developmental Biology, 10, 860502.

Ijabi, J., Roozehdar, P., Afrisham, R., Moradi-Sardareh, H., Polizzi, N., Jasoni, C. L., ... & Oraibi, B. A. M. (2022). Apoptosis in hypoxic mice influenced by miR-138-siRNAs-HIF-1α and miR-21-siRNAs-HVCN1.

Wang, F., Ma, X. R., Wu, Y., Xu, Y. C., Gu, H. M., Wang, D. X., ... & Zhao, J. W. (2021). Neutralization of Hv1/HVCN1 with antibody enhances microglia/macrophages myelin clearance by promoting their migration in the brain. Frontiers in Cellular Neuroscience, 15, 768059.

Delgado-Bermúdez, A., Mateo-Otero, Y., Llavanera, M., Bonet, S., Yeste, M., & Pinart, E. (2021). HVCN1 but not potassium channels are related to mammalian sperm cryotolerance. International Journal of Molecular Sciences, 22(4), 1646.

Yeste, M., Llavanera, M., Mateo-Otero, Y., Catalán, J., Bonet, S., & Pinart, E. (2020). HVCN1 channels are relevant for the maintenance of sperm motility during in vitro capacitation of pig spermatozoa. International Journal of Molecular Sciences, 21(9), 3255.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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