Mouse Anti-IAV H1N1 HA Recombinant Antibody (CBMW-H1043) (CBMAB-V208-1193-FY)

Name: Mouse Anti-IAV H1N1 HA Recombinant Antibody (CBMW-H1043)
SKU: CBMAB-V208-1193-FY
Rating: 5 (1 reviews)

See all IAV H1N1 HA antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBMW-H1043

Application

WB, ELISA

Immunogen

A/New Caledonia/20/99

Specificity

IAV

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Influenza A Virus Hemagglutinin H1 Ha

Introduction

Influenza A virus causes influenza in birds and some mammals, and is the only species of the Alphainfluenzavirus genus of the Orthomyxoviridae family of viruses.

UniProt ID

Q6WG00

Alternative Names

Influenza A Virus Hemagglutinin H1 HA

Function

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.

Biological Process

Clathrin-dependent endocytosis of virus by host cell Source: UniProtKB-UniRule
Fusion of virus membrane with host endosome membrane Source: UniProtKB-UniRule
Fusion of virus membrane with host plasma membrane Source: InterPro
Viral budding from plasma membrane Source: UniProtKB-UniRule
Virion attachment to host cell Source: UniProtKB-KW

Cellular Location

Virion membrane; Host apical cell membrane. Targeted to the apical plasma membrane in epithelial polarized cells through a signal present in the transmembrane domain. Associated with glycosphingolipid- and cholesterol-enriched detergent-resistant lipid rafts.

Topology

Extracellular: 18-528
Helical: 529-549
Cytoplasmic: 550-565

PTM

Palmitoylated.
In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. One identified protease that may be involved in this process is secreted in lungs by Clara cells.

Kim, J. H., Shin, J. H., Cho, C. H., Hwang, J., Kweon, D. H., Park, T. J., ... & Park, J. P. (2022). Dual synergistic response for the electrochemical detection of H1N1 virus and viral proteins using high affinity peptide receptors. Talanta, 248, 123613.

Cong, Y., Sun, Y., Deng, X., Yu, H., Lian, X., & Cong, Y. (2022). A SYBR Green-based real-time RT-PCR assay to differentiate the H1N1 influenza virus lineages. Journal of Virological Methods, 300, 114387.

Hu, M., Jones, J. C., Banoth, B., Ojha, C. R., Crumpton, J. C., Kercher, L., ... & Russell, C. J. (2022). Swine H1N1 influenza virus variants with enhanced polymerase activity and HA stability promote airborne transmission in ferrets. Journal of Virology, 96(7), e00100-22.

Zell, R., Groth, M., Krumbholz, A., Lange, J., Philipps, A., & Dürrwald, R. (2020). Cocirculation of swine H1N1 influenza A virus lineages in Germany. Viruses, 12(7), 762.

Bavagnoli, L., Campanini, G., Forte, M., Ceccotti, G., Percivalle, E., Bione, S., ... & Maga, G. (2019). Identification of a novel antiviral micro-RNA targeting the NS1 protein of the H1N1 pandemic human influenza virus and a corresponding viral escape mutation. Antiviral Research, 171, 104593.

Wen, F., Li, L., Zhao, N., Chiang, M. J., Xie, H., Cooley, J., ... & Wan, X. F. (2018). A Y161F hemagglutinin substitution increases thermostability and improves yields of 2009 H1N1 influenza A virus in cells. Journal of virology, 92(2), 10-1128.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-IAV H1N1 HA Recombinant Antibody (CBMW-H1043)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-IAV H1N1 HA Recombinant Antibody (CBMW-H1042) (CAT#: CBMAB-V208-1192-FY)

Rabbit Anti-IAV H1N1 HA Recombinant Antibody (CBMW-H1156) (CAT#: CBMAB-V208-1318-FY)

Mouse Anti-IAV H1N1 HA Recombinant Antibody (CBMW-H1152) (CAT#: CBMAB-V208-1314-FY)

Mouse Anti-IAV H1N1 HA Recombinant Antibody (CBMW-H1153) (CAT#: CBMAB-V208-1315-FY)

Mouse Anti-IAV H1N1 HA Recombinant Antibody (CBMW-H1150) (CAT#: CBMAB-V208-1312-FY)

Rabbit Anti-IAV H1N1 HA Recombinant Antibody (CBMW-H1158) (CAT#: CBMAB-V208-1320-FY)

Mouse Anti-IAV H1N1 HA (AA 18-530) Recombinant Antibody (CBMW-H0350) (CAT#: CBMAB-V208-0396-FY)

Mouse Anti-IAV H1N1 HA (AA 18-344) Recombinant Antibody (CBMW-H0341) (CAT#: CBMAB-V208-0387-FY)

Mouse Anti-IAV H1N1 HA Recombinant Antibody (CBMW-H1154) (CAT#: CBMAB-V208-1316-FY)

Mouse Anti-IAV H1N1 HA Recombinant Antibody (CBMW-H1330) (CAT#: CBMAB-V208-1520-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry