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Mouse Anti-KDM1B Recombinant Antibody (5E10C1) (CBMAB-A4531-YC)

Provided herein is a Mouse monoclonal antibody against Human Lysine Demethylase 1B. The antibody can be used for immunoassay techniques, such as WB, FC, ELISA, IHC.
See all KDM1B antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
5E10C1
Antibody Isotype
IgG
Application
WB, FC, ELISA, IHC

Basic Information

Immunogen
Purified recombinant fragment of human AOF1 (aa 6-129) expressed in E. Coli
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Lysine Demethylase 1B
Introduction
Flavin-dependent histone demethylases, such as KDM1B, regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function.
Entrez Gene ID
221656
UniProt ID
Q8NB78
Alternative Names
AOF1; C6orf193; LSD2; bA204B7.3; dJ298J15.2
Function
Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4.
Biological Process
DNA methylation involved in gamete generationIEA:Ensembl
Histone H3-K4 demethylationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Regulation of DNA methylationBy SimilarityISS:UniProtKB
Regulation of gene expression by genetic imprintingBy SimilarityISS:UniProtKB
Cellular Location
Nucleus

Anvar, Z., Chakchouk, I., Sharif, M., Mahadevan, S., Nasiotis, E. T., Su, L., ... & Van den Veyver, I. B. (2023). Loss of the Maternal Effect Gene Nlrp2 Alters the Transcriptome of Ovulated Mouse Oocytes and Impacts Expression of Histone Demethylase KDM1B. Reproductive Sciences, 1-14.

Musella, M., Guarracino, A., Manduca, N., Galassi, C., Ruggiero, E., Potenza, A., ... & Sistigu, A. (2022). Type I IFNs promote cancer cell stemness by triggering the epigenetic regulator KDM1B. Nature Immunology, 23(9), 1379-1392.

Hou, C., Ye, Z., Yang, S., Jiang, Z., Wang, J., & Wang, E. (2022). Lysine demethylase 1B (Kdm1b) enhances somatic reprogramming through inducing pluripotent gene expression and promoting cell proliferation. Experimental Cell Research, 420(1), 113339.

Lea, G. (2022). Role of Histone Lysine Demethylase, KDM1B, in Trophoblast Stem Cell Self-Renewal and Differentiation (Doctoral dissertation).

Cai, S., Wang, J., Zeng, W., Cheng, X., Liu, L., & Li, W. (2020). Lysine-specific histone demethylase 1B (LSD2/KDM1B) represses p53 expression to promote proliferation and inhibit apoptosis in colorectal cancer through LSD2-mediated H3K4me2 demethylation. Aging (albany NY), 12(14), 14990.

Wu, R. J., Zou, Y., Ma, X. D., & Zheng, R. J. (2020). Expression and clinical significance of MiR-215 and KDM1B in patients with diffuse large B cell lymphoma. Zhongguo shi yan xue ye xue za zhi, 28(5), 1570-1577.

Wang, Y., Sun, L., Luo, Y., & He, S. (2019). Knockdown of KDM1B inhibits cell proliferation and induces apoptosis of pancreatic cancer cells. Pathology-Research and Practice, 215(5), 1054-1060.

Lee, Y. K., Lim, J., Yoon, S. Y., Joo, J. C., Park, S. J., & Park, Y. J. (2019). Promotion of cell death in cisplatin-resistant ovarian cancer cells through KDM1B-DCLRE1B modulation. International Journal of Molecular Sciences, 20(10), 2443.

Cui, Y., Hu, T., Chen, R., Yu, S., Dong, W., Lv, X., & Pan, C. (2018). Novel 17-bp deletion in KDM1B gene is significantly associated with testis weight in male piglet. Animal biotechnology, 29(4), 252-258.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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