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Mouse Anti-KDM1B Recombinant Antibody (10E35(6A1-1F5)) (CBMAB-0029-WJ)

This product is a mouse antibody that recognizes KDM1B. The antibody 10E35(6A1-1F5) can be used for immunoassay techniques such as: ICC, IF, IP, WB.
See all KDM1B antibodies

Summary

Host Animal
Mouse
Specificity
Human, Monkey, Mouse, Rat
Clone
10E35(6A1-1F5)
Antibody Isotype
IgG1
Application
ICC, IF, IP, WB

Basic Information

Immunogen
Recombinant protein from the sequence of human JMJD1B.
Specificity
Human, Monkey, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Lysine Demethylase 1B
Introduction
KDM1B (Lysine Demethylase 1B) is a Protein Coding gene. The protein regulates histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function. An important paralog of this gene is KDM1A.
Entrez Gene ID
Human221656
Mouse218214
Rat306819
Monkey704663
UniProt ID
HumanQ8NB78
MouseQ8CIG3
RatD3ZP89
MonkeyA0A0D9R5M4
Alternative Names
lysine demethylase 1B
Function
Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4.
Biological Process
DNA methylation involved in gamete generationIEA:Ensembl
Histone H3-K4 demethylationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Regulation of DNA methylationBy SimilarityISS:UniProtKB
Regulation of gene expression by genetic imprintingBy SimilarityISS:UniProtKB
Cellular Location
Nucleus

Anvar, Z., Chakchouk, I., Sharif, M., Mahadevan, S., Nasiotis, E. T., Su, L., ... & Van den Veyver, I. B. (2023). Loss of the Maternal Effect Gene Nlrp2 Alters the Transcriptome of Ovulated Mouse Oocytes and Impacts Expression of Histone Demethylase KDM1B. Reproductive Sciences, 1-14.

Musella, M., Guarracino, A., Manduca, N., Galassi, C., Ruggiero, E., Potenza, A., ... & Sistigu, A. (2022). Type I IFNs promote cancer cell stemness by triggering the epigenetic regulator KDM1B. Nature Immunology, 23(9), 1379-1392.

Hou, C., Ye, Z., Yang, S., Jiang, Z., Wang, J., & Wang, E. (2022). Lysine demethylase 1B (Kdm1b) enhances somatic reprogramming through inducing pluripotent gene expression and promoting cell proliferation. Experimental Cell Research, 420(1), 113339.

Lea, G. (2022). Role of Histone Lysine Demethylase, KDM1B, in Trophoblast Stem Cell Self-Renewal and Differentiation (Doctoral dissertation).

Cai, S., Wang, J., Zeng, W., Cheng, X., Liu, L., & Li, W. (2020). Lysine-specific histone demethylase 1B (LSD2/KDM1B) represses p53 expression to promote proliferation and inhibit apoptosis in colorectal cancer through LSD2-mediated H3K4me2 demethylation. Aging (albany NY), 12(14), 14990.

Wu, R. J., Zou, Y., Ma, X. D., & Zheng, R. J. (2020). Expression and clinical significance of MiR-215 and KDM1B in patients with diffuse large B cell lymphoma. Zhongguo shi yan xue ye xue za zhi, 28(5), 1570-1577.

Wang, Y., Sun, L., Luo, Y., & He, S. (2019). Knockdown of KDM1B inhibits cell proliferation and induces apoptosis of pancreatic cancer cells. Pathology-Research and Practice, 215(5), 1054-1060.

Lee, Y. K., Lim, J., Yoon, S. Y., Joo, J. C., Park, S. J., & Park, Y. J. (2019). Promotion of cell death in cisplatin-resistant ovarian cancer cells through KDM1B-DCLRE1B modulation. International Journal of Molecular Sciences, 20(10), 2443.

Cui, Y., Hu, T., Chen, R., Yu, S., Dong, W., Lv, X., & Pan, C. (2018). Novel 17-bp deletion in KDM1B gene is significantly associated with testis weight in male piglet. Animal biotechnology, 29(4), 252-258.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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