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Mouse Anti-KDM1B Recombinant Antibody (CBT3495) (V2LY-0625-LY359)


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Tested Data
Mouse Anti-KDM1B Recombinant Antibody (CBT3495) (V2LY-0625-LY359) in ELISA
Mouse Anti-KDM1B Recombinant Antibody (CBT3495) (V2LY-0625-LY359) in ELISA
Mouse Anti-KDM1B Recombinant Antibody (CBT3495) (V2LY-0625-LY359) in WB
Mouse Anti-KDM1B Recombinant Antibody (CBT3495) (V2LY-0625-LY359) in WB
Mouse Anti-KDM1B Recombinant Antibody (CBT3495) (V2LY-0625-LY359) in WB
Mouse Anti-KDM1B Recombinant Antibody (CBT3495) (V2LY-0625-LY359) in WB
Mouse Anti-KDM1B Recombinant Antibody (CBT3495) (V2LY-0625-LY359) in IF
Mouse Anti-KDM1B Recombinant Antibody (CBT3495) (V2LY-0625-LY359) in IF
Mouse Anti-KDM1B Recombinant Antibody (CBT3495) (V2LY-0625-LY359) in IF
Mouse Anti-KDM1B Recombinant Antibody (CBT3495) (V2LY-0625-LY359) in IF
Mouse Anti-KDM1B Recombinant Antibody (CBT3495) (V2LY-0625-LY359) in IHC
Mouse Anti-KDM1B Recombinant Antibody (CBT3495) (V2LY-0625-LY359) in IHC
Mouse Anti-KDM1B Recombinant Antibody (CBT3495) (V2LY-0625-LY359) in IHC
Mouse Anti-KDM1B Recombinant Antibody (CBT3495) (V2LY-0625-LY359) in IHC
Mouse Anti-KDM1B Recombinant Antibody (CBT3495) (V2LY-0625-LY359) in FC
Mouse Anti-KDM1B Recombinant Antibody (CBT3495) (V2LY-0625-LY359) in FC

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT3495
Antibody Isotype
IgG1
Application
IHC, ICC, FC

Basic Information

Immunogen
Purified recombinant fragment of human AOF1 (AA: 6-129) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
IHC-P1:200-1:1,000
ICC1:200-1:1,000
FC1:200-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Entrez Gene ID
221656
UniProt ID
Q8NB78
Function
Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4.
Biological Process
DNA methylation involved in gamete generationIEA:Ensembl
Histone H3-K4 demethylationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Regulation of DNA methylationBy SimilarityISS:UniProtKB
Regulation of gene expression by genetic imprintingBy SimilarityISS:UniProtKB
Cellular Location
Nucleus
More Infomation

Anvar, Z., Chakchouk, I., Sharif, M., Mahadevan, S., Nasiotis, E. T., Su, L., ... & Van den Veyver, I. B. (2023). Loss of the Maternal Effect Gene Nlrp2 Alters the Transcriptome of Ovulated Mouse Oocytes and Impacts Expression of Histone Demethylase KDM1B. Reproductive Sciences, 1-14.

Musella, M., Guarracino, A., Manduca, N., Galassi, C., Ruggiero, E., Potenza, A., ... & Sistigu, A. (2022). Type I IFNs promote cancer cell stemness by triggering the epigenetic regulator KDM1B. Nature Immunology, 23(9), 1379-1392.

Hou, C., Ye, Z., Yang, S., Jiang, Z., Wang, J., & Wang, E. (2022). Lysine demethylase 1B (Kdm1b) enhances somatic reprogramming through inducing pluripotent gene expression and promoting cell proliferation. Experimental Cell Research, 420(1), 113339.

Lea, G. (2022). Role of Histone Lysine Demethylase, KDM1B, in Trophoblast Stem Cell Self-Renewal and Differentiation (Doctoral dissertation).

Cai, S., Wang, J., Zeng, W., Cheng, X., Liu, L., & Li, W. (2020). Lysine-specific histone demethylase 1B (LSD2/KDM1B) represses p53 expression to promote proliferation and inhibit apoptosis in colorectal cancer through LSD2-mediated H3K4me2 demethylation. Aging (albany NY), 12(14), 14990.

Wu, R. J., Zou, Y., Ma, X. D., & Zheng, R. J. (2020). Expression and clinical significance of MiR-215 and KDM1B in patients with diffuse large B cell lymphoma. Zhongguo shi yan xue ye xue za zhi, 28(5), 1570-1577.

Wang, Y., Sun, L., Luo, Y., & He, S. (2019). Knockdown of KDM1B inhibits cell proliferation and induces apoptosis of pancreatic cancer cells. Pathology-Research and Practice, 215(5), 1054-1060.

Lee, Y. K., Lim, J., Yoon, S. Y., Joo, J. C., Park, S. J., & Park, Y. J. (2019). Promotion of cell death in cisplatin-resistant ovarian cancer cells through KDM1B-DCLRE1B modulation. International Journal of Molecular Sciences, 20(10), 2443.

Cui, Y., Hu, T., Chen, R., Yu, S., Dong, W., Lv, X., & Pan, C. (2018). Novel 17-bp deletion in KDM1B gene is significantly associated with testis weight in male piglet. Animal biotechnology, 29(4), 252-258.

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For research use only. Not intended for any clinical use.

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