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Rabbit Anti-LIMK2 Recombinant Antibody (CBYJL-1725) (CBMAB-L1619-YJ)

Provided herein is a Rabbit monoclonal antibody, which binds to Lim Domain Kinase 2 (LIMK2). The antibody can be used for immunoassay techniques, such as WB, FC.
See all LIMK2 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Monkey, Mouse
Clone
CBYJL-1725
Antibody Isotype
IgG
Application
WB, FC

Basic Information

Specificity
Human, Monkey, Mouse
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
LIM domain kinase 2
Introduction
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene.
Entrez Gene ID
Human3985
Mouse16886
Monkey716614
UniProt ID
HumanP53671
MouseO54785
MonkeyA0A5F8ARW0
Function
Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics (PubMed:10436159, PubMed:11018042).
Acts downstream of several Rho family GTPase signal transduction pathways (PubMed:10436159, PubMed:11018042).
Involved in astral microtubule organization and mitotic spindle orientation during early stages of mitosis by mediating phosphorylation of TPPP (PubMed:22328514).
Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro (PubMed:8537403).
Suppresses ciliogenesis via multiple pathways; phosphorylation of CFL1, suppression of directional trafficking of ciliary vesicles to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1 (PubMed:25849865).
Biological Process
Actin cytoskeleton organizationManual Assertion Based On ExperimentIBA:GO_Central
Astral microtubule organizationManual Assertion Based On ExperimentIDA:UniProtKB
Cornea development in camera-type eyeIEA:Ensembl
Establishment of vesicle localizationManual Assertion Based On ExperimentIMP:UniProtKB
Head developmentIEA:Ensembl
Negative regulation of cilium assemblyManual Assertion Based On ExperimentIMP:UniProtKB
PhosphorylationManual Assertion Based On ExperimentTAS:UniProtKB
Positive regulation of protein localization to nucleusManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of protein phosphorylationManual Assertion Based On ExperimentIMP:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
SpermatogenesisIEA:Ensembl
Cellular Location
Cytoplasm, cytoskeleton, spindle
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Isoform LIMK2a:
Cytoplasm
Nucleus
Isoform LIMK2b:
Cytoplasm
Cytoplasm, perinuclear region
Nucleus
Mainly present in the cytoplasm and is scarcely translocated to the nucleus.
PTM
Phosphorylated on serine and/or threonine residues by ROCK1.

Shah, K., & Cook, M. (2023). LIMK2: A Multifaceted kinase with pleiotropic roles in human physiology and pathologies. Cancer Letters, 216207.

Villalonga, E., Mosrin, C., Normand, T., Girardin, C., Serrano, A., Žunar, B., ... & Vallée, B. (2023). LIM Kinases, LIMK1 and LIMK2, Are Crucial Node Actors of the Cell Fate: Molecular to Pathological Features. Cells, 12(5), 805.

Malvi, P., Reddy, D. S., Kumar, R., Chava, S., Burela, S., Parajuli, K., ... & Wajapeyee, N. (2023). LIMK2 promotes melanoma tumor growth and metastasis through G3BP1-ESM1 pathway-mediated apoptosis inhibition. Oncogene, 42(18), 1478-1491.

Nikhil, K., Haymour, H. S., Kamra, M., & Shah, K. (2021). Phosphorylation-dependent regulation of SPOP by LIMK2 promotes castration-resistant prostate cancer. British Journal of Cancer, 124(5), 995-1008.

Sooreshjani, M. A., Nikhil, K., Kamra, M., Nguyen, D. N., Kumar, D., & Shah, K. (2021). LIMK2-NKX3. 1 engagement promotes castration-resistant prostate cancer. Cancers, 13(10), 2324.

Malvi, P., Janostiak, R., Chava, S., Manrai, P., Yoon, E., Singh, K., ... & Wajapeyee, N. (2020). LIMK2 promotes the metastatic progression of triple-negative breast cancer by activating SRPK1. Oncogenesis, 9(8), 77.

Mao, R., Deng, R., Wei, Y., Han, L., Meng, Y., Xie, W., & Jia, Z. (2019). LIMK1 and LIMK2 regulate cortical development through affecting neural progenitor cell proliferation and migration. Molecular Brain, 12(1), 1-11.

Nikhil, K., Chang, L., Viccaro, K., Jacobsen, M., McGuire, C., Satapathy, S. R., ... & Shah, K. (2019). Identification of LIMK2 as a therapeutic target in castration resistant prostate cancer. Cancer letters, 448, 182-196.

Xiong, M., Chen, L., Zhou, L., Ding, Y., Kazobinka, G., Chen, Z., & Hou, T. (2019). NUDT21 inhibits bladder cancer progression through ANXA2 and LIMK2 by alternative polyadenylation. Theranostics, 9(24), 7156.

Vallée, B., Cuberos, H., Doudeau, M., Godin, F., Gosset, D., Vourc'h, P., ... & Bénédetti, H. (2018). LIMK2-1, a new isoform of human LIMK2, regulates actin cytoskeleton remodeling via a different signaling pathway than that of its two homologs, LIMK2a and LIMK2b. Biochemical Journal, 475(23), 3745-3761.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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