Mouse Anti-LIMK2 Recombinant Antibody (2H2-E11) (CBMAB-L1618-YJ)

Name: Mouse Anti-LIMK2 Recombinant Antibody (2H2-E11)
SKU: CBMAB-L1618-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2H2-E11

Application

ELISA, IF, WB

Immunogen

Full length recombinant corresponding to aa 1-686 from human LIMK2 (AAH13051) with GST tag. MW of the GST tag alone is 26kD.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.2

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 1-686

More Infomation

Target

Full Name

LIM domain kinase 2

Introduction

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene.

Entrez Gene ID

3985

UniProt ID

P53671

Function

Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics (PubMed:10436159, PubMed:11018042).
Acts downstream of several Rho family GTPase signal transduction pathways (PubMed:10436159, PubMed:11018042).
Involved in astral microtubule organization and mitotic spindle orientation during early stages of mitosis by mediating phosphorylation of TPPP (PubMed:22328514).
Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro (PubMed:8537403).
Suppresses ciliogenesis via multiple pathways; phosphorylation of CFL1, suppression of directional trafficking of ciliary vesicles to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1 (PubMed:25849865).

Biological Process

Actin cytoskeleton organizationManual Assertion Based On ExperimentIBA:GO_Central
Astral microtubule organizationManual Assertion Based On ExperimentIDA:UniProtKB
Cornea development in camera-type eyeIEA:Ensembl
Establishment of vesicle localizationManual Assertion Based On ExperimentIMP:UniProtKB
Head developmentIEA:Ensembl
Negative regulation of cilium assemblyManual Assertion Based On ExperimentIMP:UniProtKB
PhosphorylationManual Assertion Based On ExperimentTAS:UniProtKB
Positive regulation of protein localization to nucleusManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of protein phosphorylationManual Assertion Based On ExperimentIMP:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
SpermatogenesisIEA:Ensembl

Cellular Location

Cytoplasm, cytoskeleton, spindle
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Isoform LIMK2a:
Cytoplasm
Nucleus
Isoform LIMK2b:
Cytoplasm
Cytoplasm, perinuclear region
Nucleus
Mainly present in the cytoplasm and is scarcely translocated to the nucleus.

PTM

Phosphorylated on serine and/or threonine residues by ROCK1.

Shah, K., & Cook, M. (2023). LIMK2: A Multifaceted kinase with pleiotropic roles in human physiology and pathologies. Cancer Letters, 216207.

Villalonga, E., Mosrin, C., Normand, T., Girardin, C., Serrano, A., Žunar, B., ... & Vallée, B. (2023). LIM Kinases, LIMK1 and LIMK2, Are Crucial Node Actors of the Cell Fate: Molecular to Pathological Features. Cells, 12(5), 805.

Malvi, P., Reddy, D. S., Kumar, R., Chava, S., Burela, S., Parajuli, K., ... & Wajapeyee, N. (2023). LIMK2 promotes melanoma tumor growth and metastasis through G3BP1-ESM1 pathway-mediated apoptosis inhibition. Oncogene, 42(18), 1478-1491.

Nikhil, K., Haymour, H. S., Kamra, M., & Shah, K. (2021). Phosphorylation-dependent regulation of SPOP by LIMK2 promotes castration-resistant prostate cancer. British Journal of Cancer, 124(5), 995-1008.

Sooreshjani, M. A., Nikhil, K., Kamra, M., Nguyen, D. N., Kumar, D., & Shah, K. (2021). LIMK2-NKX3. 1 engagement promotes castration-resistant prostate cancer. Cancers, 13(10), 2324.

Malvi, P., Janostiak, R., Chava, S., Manrai, P., Yoon, E., Singh, K., ... & Wajapeyee, N. (2020). LIMK2 promotes the metastatic progression of triple-negative breast cancer by activating SRPK1. Oncogenesis, 9(8), 77.

Mao, R., Deng, R., Wei, Y., Han, L., Meng, Y., Xie, W., & Jia, Z. (2019). LIMK1 and LIMK2 regulate cortical development through affecting neural progenitor cell proliferation and migration. Molecular Brain, 12(1), 1-11.

Nikhil, K., Chang, L., Viccaro, K., Jacobsen, M., McGuire, C., Satapathy, S. R., ... & Shah, K. (2019). Identification of LIMK2 as a therapeutic target in castration resistant prostate cancer. Cancer letters, 448, 182-196.

Xiong, M., Chen, L., Zhou, L., Ding, Y., Kazobinka, G., Chen, Z., & Hou, T. (2019). NUDT21 inhibits bladder cancer progression through ANXA2 and LIMK2 by alternative polyadenylation. Theranostics, 9(24), 7156.

Vallée, B., Cuberos, H., Doudeau, M., Godin, F., Gosset, D., Vourc'h, P., ... & Bénédetti, H. (2018). LIMK2-1, a new isoform of human LIMK2, regulates actin cytoskeleton remodeling via a different signaling pathway than that of its two homologs, LIMK2a and LIMK2b. Biochemical Journal, 475(23), 3745-3761.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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