Human Recombinant LIMK2, Active protein, GST Tag (V2LY-0526-LY5316)

Name: Human Recombinant LIMK2, Active protein, GST Tag
SKU: V2LY-0526-LY5316
Rating: 5 (1 reviews)

See all LIMK2 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant LIMK2, Active protein, GST Tag consist of Amino Acid: Full Length and predicts a molecular mass of 96 kDa.

Molecule Mass

96 kDa

Sequence

Amino Acid: Full Length

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

LIM domain kinase 2

Function

Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics (PubMed:10436159, PubMed:11018042).
Acts downstream of several Rho family GTPase signal transduction pathways (PubMed:10436159, PubMed:11018042).
Involved in astral microtubule organization and mitotic spindle orientation during early stages of mitosis by mediating phosphorylation of TPPP (PubMed:22328514).
Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro (PubMed:8537403).
Suppresses ciliogenesis via multiple pathways; phosphorylation of CFL1, suppression of directional trafficking of ciliary vesicles to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1 (PubMed:25849865).

Biological Process

Actin cytoskeleton organizationManual Assertion Based On ExperimentIBA:GO_Central
Astral microtubule organizationManual Assertion Based On ExperimentIDA:UniProtKB
Cornea development in camera-type eyeIEA:Ensembl
Establishment of vesicle localizationManual Assertion Based On ExperimentIMP:UniProtKB
Head developmentIEA:Ensembl
Negative regulation of cilium assemblyManual Assertion Based On ExperimentIMP:UniProtKB
PhosphorylationManual Assertion Based On ExperimentTAS:UniProtKB
Positive regulation of protein localization to nucleusManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of protein phosphorylationManual Assertion Based On ExperimentIMP:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
SpermatogenesisIEA:Ensembl

Cellular Location

Cytoplasm, cytoskeleton, spindle
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Isoform LIMK2a:
Cytoplasm
Nucleus
Isoform LIMK2b:
Cytoplasm
Cytoplasm, perinuclear region
Nucleus
Mainly present in the cytoplasm and is scarcely translocated to the nucleus.

PTM

Phosphorylated on serine and/or threonine residues by ROCK1.

Shah, K., & Cook, M. (2023). LIMK2: A Multifaceted kinase with pleiotropic roles in human physiology and pathologies. Cancer Letters, 216207.

Villalonga, E., Mosrin, C., Normand, T., Girardin, C., Serrano, A., Žunar, B., ... & Vallée, B. (2023). LIM Kinases, LIMK1 and LIMK2, Are Crucial Node Actors of the Cell Fate: Molecular to Pathological Features. Cells, 12(5), 805.

Malvi, P., Reddy, D. S., Kumar, R., Chava, S., Burela, S., Parajuli, K., ... & Wajapeyee, N. (2023). LIMK2 promotes melanoma tumor growth and metastasis through G3BP1-ESM1 pathway-mediated apoptosis inhibition. Oncogene, 42(18), 1478-1491.

Nikhil, K., Haymour, H. S., Kamra, M., & Shah, K. (2021). Phosphorylation-dependent regulation of SPOP by LIMK2 promotes castration-resistant prostate cancer. British Journal of Cancer, 124(5), 995-1008.

Sooreshjani, M. A., Nikhil, K., Kamra, M., Nguyen, D. N., Kumar, D., & Shah, K. (2021). LIMK2-NKX3. 1 engagement promotes castration-resistant prostate cancer. Cancers, 13(10), 2324.

Malvi, P., Janostiak, R., Chava, S., Manrai, P., Yoon, E., Singh, K., ... & Wajapeyee, N. (2020). LIMK2 promotes the metastatic progression of triple-negative breast cancer by activating SRPK1. Oncogenesis, 9(8), 77.

Mao, R., Deng, R., Wei, Y., Han, L., Meng, Y., Xie, W., & Jia, Z. (2019). LIMK1 and LIMK2 regulate cortical development through affecting neural progenitor cell proliferation and migration. Molecular Brain, 12(1), 1-11.

Nikhil, K., Chang, L., Viccaro, K., Jacobsen, M., McGuire, C., Satapathy, S. R., ... & Shah, K. (2019). Identification of LIMK2 as a therapeutic target in castration resistant prostate cancer. Cancer letters, 448, 182-196.

Xiong, M., Chen, L., Zhou, L., Ding, Y., Kazobinka, G., Chen, Z., & Hou, T. (2019). NUDT21 inhibits bladder cancer progression through ANXA2 and LIMK2 by alternative polyadenylation. Theranostics, 9(24), 7156.

Vallée, B., Cuberos, H., Doudeau, M., Godin, F., Gosset, D., Vourc'h, P., ... & Bénédetti, H. (2018). LIMK2-1, a new isoform of human LIMK2, regulates actin cytoskeleton remodeling via a different signaling pathway than that of its two homologs, LIMK2a and LIMK2b. Biochemical Journal, 475(23), 3745-3761.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant LIMK2, Active protein, GST Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Rabbit Anti-LIMK2 Recombinant Antibody (CBYJL-1725) (CAT#: CBMAB-L1619-YJ)

Mouse Anti-LIMK2 Recombinant Antibody (2H2-E11) (CAT#: CBMAB-L1618-YJ)

Rat Anti-LIMK2 Recombinant Antibody (CBYJL-1724) (CAT#: CBMAB-L1617-YJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry