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Mouse Anti-MAP3K3 Recombinant Antibody (1H3) (CBMAB-A5208-LY)

The product is antibody recognizes MAP3K3. The antibody 1H3 immunoassay techniques such as: WB, ELISA.
See all MAP3K3 antibodies
Published Data
Fig.1 Expression of MEKK3 was Detected in Cervical Cancer by Western Blotting.
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Fig.2 The Expression of MEKK3 and Survivin in Cervical Cancer, CIN, and Chronic Cervicitis.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
1H3
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
MAP3K3 (AAH10464.1, 1 a.a. ~ 89 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Mitogen-Activated Protein Kinase Kinase Kinase 3
Introduction
This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]
Entrez Gene ID
4215
UniProt ID
Q99759
Alternative Names
MAPKKK3; MEKK3
Function
Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.
Biological Process
Blood vessel developmentIEA:Ensembl
Intracellular signal transductionManual Assertion Based On ExperimentIDA:MGI
MAPK cascadeManual Assertion Based On ExperimentTAS:ProtInc
Negative regulation of cellular senescenceManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of cell migration involved in sprouting angiogenesisManual Assertion Based On ExperimentIGI:BHF-UCL
Positive regulation of cell proliferation in bone marrowManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of I-kappaB kinase/NF-kappaB signalingManual Assertion Based On ExperimentIEP:UniProtKB
Positive regulation of p38MAPK cascadeIEA:Ensembl
Protein autophosphorylationManual Assertion Based On ExperimentIDA:MGI
Cellular Location
Cytoplasm
Cytosol
PTM
Phosphorylation at Ser-166 and Ser-337 by SGK1 inhibits its activity.

Ren, J., Huang, Y., Ren, Y., Tu, T., Qiu, B., Ai, D., ... & Ge, W. P. (2023). Somatic variants of MAP3K3 are sufficient to cause cerebral and spinal cord cavernous malformations. Brain, awad104.

Zhou, H., Yang, D., Cheng, H. S., McCoy, M. G., Pérez-Cremades, D., Haemmig, S., ... & Feinberg, M. W. (2022). miR-181b regulates vascular endothelial aging by modulating a MAP3K3 signaling pathway. FASEB journal: official publication of the Federation of American Societies for Experimental Biology, 36(6), e22353.

Huo, R., Wang, J., Sun, Y. F., Weng, J. C., Li, H., Jiao, Y. M., ... & Cao, Y. (2022). Simplex cerebral cavernous malformations with MAP3K3 mutation have distinct clinical characteristics. Frontiers in Neurology, 13, 946324.

Weng, J., Yang, Y., Song, D., Huo, R., Li, H., Chen, Y., ... & Cao, Y. (2021). Somatic MAP3K3 mutation defines a subclass of cerebral cavernous malformation. The American Journal of Human Genetics, 108(5), 942-950.

Yuan, Q., Basit, A., Liang, W., Qu, R., Luan, Y., Ren, C., ... & Wu, D. (2021). Pazopanib ameliorates acute lung injuries via inhibition of MAP3K2 and MAP3K3. Science translational medicine, 13(591), eabc2499.

Hong, T., Xiao, X., Ren, J., Cui, B., Zong, Y., Zou, J., ... & Wang, Y. (2021). Somatic MAP3K3 and PIK3CA mutations in sporadic cerebral and spinal cord cavernous malformations. Brain, 144(9), 2648-2658.

Lv, Y., Huang, Y., Xu, M., Heng, B. C., Yang, C., Cao, C., ... & Deng, X. (2020). The miR‐193a‐3p‐MAP3k3 signaling axis regulates substrate topography‐induced osteogenesis of bone marrow stem cells. Advanced science, 7(1), 1901412.

Gui, D., & Cao, H. (2020). Long non-coding RNA CDKN2B-AS1 promotes osteosarcoma by increasing the expression of MAP3K3 via sponging miR-4458. In Vitro Cellular & Developmental Biology-Animal, 56(1), 24-33.

Tang, H., Lv, W., Sun, W., Bi, Q., & Hao, Y. (2019). miR‑505 inhibits cell growth and EMT by targeting MAP3K3 through the AKT‑NFκB pathway in NSCLC cells. International journal of molecular medicine, 43(3), 1203-1216.

Zhang, Y., Wang, S. S., Tao, L., Pang, L. J., Zou, H., Liang, W. H., ... & Li, F. (2019). Overexpression of MAP3K3 promotes tumour growth through activation of the NF-κB signalling pathway in ovarian carcinoma. Scientific Reports, 9(1), 8401.

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For research use only. Not intended for any clinical use.

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