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Mouse Anti-MAP4K1 (AA 278-377) Recombinant Antibody (CBFYM-1547) (CBMAB-M1712-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-1547
Antibody Isotype
IgG1, k
Application
ELISA, WB

Basic Information

Immunogen
Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: GLNRGLILDL LDKLKNPGKG PSIGDIEDEE PELPPAIPRR IRSTHRSSSL GIPDADCCRR HMEFRKLRGM ETRPPANTAR LQPPRDLRSS SPRKQLSESS
Specificity
Human
Antibody Isotype
IgG1, k
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 278-377

Target

Full Name
mitogen-activated protein kinase kinase kinase kinase 1
Introduction
MAP4K1 is a Protein Coding gene. Among its related pathways are Kit receptor signaling pathway and TGF-Beta Pathway. Gene Ontology annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is MAP4K5.
Entrez Gene ID
11184
UniProt ID
Q92918
Alternative Names
Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1; Hematopoietic Progenitor Kinase 1; MAPK/ERK Kinase Kinase Kinase 1; MEK Kinase Kinase 1; EC 2.7.11.1; MEKKK 1; HPK1; Hematopoietic Progenitor Kinase; EC 2.7.11
Function
Serine/threonine-protein kinase, which may play a role in the response to environmental stress (PubMed:24362026).
Appears to act upstream of the JUN N-terminal pathway (PubMed:8824585).
May play a role in hematopoietic lineage decisions and growth regulation (PubMed:8824585, PubMed:24362026).
Able to autophosphorylate (PubMed:8824585).
Together with CLNK, it enhances CD3-triggered activation of T-cells and subsequent IL2 production (By similarity).
Biological Process
Cell population proliferationManual Assertion Based On ExperimentIMP:UniProtKB
Cellular response to phorbol 13-acetate 12-myristateManual Assertion Based On ExperimentIDA:UniProtKB
JNK cascadeManual Assertion Based On ExperimentIDA:UniProtKB
Peptidyl-serine phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of MAPK cascadeManual Assertion Based On ExperimentIDA:UniProtKB
Protein autophosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Membrane
PTM
Autophosphorylates: phosphorylation promotes ubiquitination by the Cul7-RING(FBXW8) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
Tyrosine-phosphorylated after activation of hemopoietic cells.
Ubiquitinated by the Cul7-RING(FBXW8) ubiquitin-protein ligase complex following autophosphorylation, leading to its degradation by the proteasome.
More Infomation

Knight, T. E., Edwards, H., Taub, J. W., & Ge, Y. (2021). MAP4K1 expression is a novel resistance mechanism and independent prognostic marker in AML-but can be overcome via targeted inhibition. EBioMedicine, 70.

He, T. S., Huang, J., Chen, T., Zhang, Z., Cai, K., Yu, J., & Xu, L. G. (2021). The Kinase MAP4K1 Inhibits Cytosolic RNA-Induced Antiviral Signaling by Promoting Proteasomal Degradation of TBK1/IKKε. Microbiology Spectrum, 9(3), e01458-21.

Ling, Q., Li, F., Zhang, X., Mao, S., Lin, X., Pan, J., ... & Jin, J. (2021). MAP4K1 functions as a tumor promotor and drug mediator for AML via modulation of DNA damage/repair system and MAPK pathway. EBioMedicine, 69.

Faia, K., Toso, A., Fetalvero, K., Roche, M., Bench, S., O'Hearn, E., ... & Brubaker, J. (2021). MAP4K1 inhibition enhances immune cell activation and anti-tumor immunity in preclinical tumor models. Cancer Research, 81(13_Supplement), 1717-1717.

Leder, G., Carretero, R., Mowat, J., Berndt, S., Neuhaus, R., Font, N. A., ... & Offringa, R. (2021). Enhancement of anti-tumor T-cell immunity by means of an oral small molecule targeting the intracellular immune checkpoint MAP4K1. Cancer Research, 81(13_Supplement), 1722-1722.

Wu, Q., Ma, J., Meng, W., & Hui, P. (2020). DLX6-AS1 promotes cell proliferation, migration and EMT of gastric cancer through FUS-regulated MAP4K1. Cancer Biology & Therapy, 21(1), 17-25.

Bai, Y., Liu, X., Qi, X., Liu, X., Peng, F., Li, H., ... & Shao, S. (2019). PDIA6 modulates apoptosis and autophagy of non-small cell lung cancer cells via the MAP4K1/JNK signaling pathway. EBioMedicine, 42, 311-325.

Lei, J. J., Li, H. Q., Mo, Z. H., Liu, K. J., Zhu, L. J., Li, C. Y., ... & Zhang, L. (2019). Long noncoding RNA CDKN2B‐AS1 interacts with transcription factor BCL11A to regulate progression of cerebral infarction through mediating MAP4K1 transcription. The FASEB Journal, 33(6), 7037-7048.

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For research use only. Not intended for any clinical use.

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