Mouse Anti-MICA Recombinant Antibody (CBFYM-2208) (CBMAB-M2391-FY)

Name: Mouse Anti-MICA Recombinant Antibody (CBFYM-2208)
SKU: CBMAB-M2391-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-2208

Application

FC, IHC-P

Immunogen

BaF3 mouse pro-B cell line transfected with human MICA

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, trehalose

Concentration

0.5 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

MHC Class I Polypeptide-Related Sequence A

Introduction

This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants.

Entrez Gene ID

100507436

UniProt ID

Q29983

Alternative Names

MHC Class I Polypeptide-Related Sequence A; PERB11.1; MIC-A; Truncated MHC Class I Polypeptide-Related Sequence A; MHC Class I Chain-Related Protein A; Stress Inducible Class I Homolog; MHC Class I Related Sequence A; MHC Class I Related Chain A; HLA Class I Antigen

Function

Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.

Biological Process

Cellular response to DNA damage stimulus Source: UniProtKB
Cytolysis Source: UniProtKB-KW
Defense response to bacterium Source: UniProtKB
Defense response to virus Source: UniProtKB
Gamma-delta T cell activation Source: UniProtKB
Immune response to tumor cell Source: UniProtKB
Natural killer cell mediated cytotoxicity Source: UniProtKB
Negative regulation of natural killer cell activation Source: CACAO
Negative regulation of natural killer cell mediated cytotoxicity Source: CACAO
Response to heat Source: UniProtKB
T cell mediated cytotoxicity Source: UniProtKB

Cellular Location

Cytoplasm
Plasma membrane
Cell membrane
Note: Expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Infection with human adenovirus 5 suppresses cell surface expression due to the adenoviral E3-19K protein which causes retention in the endoplasmic reticulum.

Involvement in disease

Anti-MICA antibodies and ligand shedding are involved in the progression of monoclonal gammopathy of undetermined significance (MGUS)to multiple myeloma.
Psoriasis 1 (PSORS1):
A common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis.
Psoriatic arthritis (PSORAS):
An inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoid like pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).

Topology

Extracellular: 24-307
Helical: 308-328
Cytoplasmic: 329-383

PTM

N-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation.
Proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation.

Ren, X., Mao, A., Tan, S., Liu, J., & Wei, X. (2022). Analysis of the association between MICA gene polymorphisms and schizophrenia. Journal of Clinical Laboratory Analysis, 36(11), e24721.

Espinoza, I., Agarwal, S., Sakiyama, M., Shenoy, V., Orr, W. S., Al Diffalha, S., ... & Gomez, C. R. (2021). Expression of MHC class I polypeptide-related sequence A (MICA) in colorectal cancer.

Closa, L., Vidal, F., Herrero, M. J., & Caro, J. L. (2021). High‐throughput genotyping of HLA‐G, HLA‐F, MICA, and MICB and analysis of frequency distributions in healthy blood donors from Catalonia. HLA, 97(5), 420-427.

Otoyama, Y., Arai, J., Goto, K., Nozawa, H., Nakagawa, R., Muroyama, R., ... & Yoshida, H. (2021). Retinoids decrease soluble MICA concentration by inhibiting the enzymatic activity of ADAM9 and ADAM10. Anticancer Research, 41(5), 2307-2320.

Gong, X., Chen, P., Ma, P., Gao, J., Yang, J., Guo, H., ... & He, Y. (2020). MICA polymorphisms associated with antithyroid drug‐induced agranulocytosis in the Chinese Han population. Immunity, Inflammation and Disease, 8(4), 695-703.

Plasil, M., Wijkmark, S., Elbers, J. P., Oppelt, J., Burger, P. A., & Horin, P. (2019). The major histocompatibility complex of Old World camelids: class I and class I‐related genes. Hla, 93(4), 203-215.

Sakiyama, M. J., Espinoza, I., Reddy, A., de Carlo, F., Kumar, A., Levenson, A. S., ... & Gomez, C. R. (2019). Race-associated expression of MHC class I polypeptide-related sequence A (MICA) in prostate cancer. Experimental and molecular pathology, 108, 173-182.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

MICA Matched Antibody Pair (735) (CAT#: APMAB-735LY)

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Isotype control

For research use only. Not intended for any clinical use.

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