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Mouse Anti-NOX4 Recombinant Antibody (CBT3766) (V2LY-0625-LY251)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT3766
Antibody Isotype
IgG1
Application
IHC, ICC, FC

Basic Information

Immunogen
Purified recombinant fragment of human NOX4 (AA: 210-310) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
IHC-P1:200-1:1,000
ICC1:200-1:1,000
FC1:200-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
NADPH Oxidase 4
Entrez Gene ID
50507
UniProt ID
Q9NPH5
Function
Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity.
Isoform 4
Involved in redox signaling in vascular cells. Constitutively and NADPH-dependently generates reactive oxygen species (ROS). Modulates the nuclear activation of ERK1/2 and the ELK1 transcription factor, and is capable of inducing nuclear DNA damage. Displays an increased activity relative to isoform 1.
Biological Process
Bone resorptionIEA:Ensembl
Cardiac muscle cell differentiationIEA:Ensembl
Cell agingBy SimilarityISS:UniProtKB
Cell morphogenesisBy SimilarityISS:UniProtKB
Cellular response to glucose stimulusIEA:Ensembl
Defense responseManual Assertion Based On ExperimentIBA:GO_Central
Gene expressionManual Assertion Based On ExperimentIMP:CACAO
Heart processManual Assertion Based On ExperimentIGI:ARUK-UCL
Homocysteine metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Inflammatory responseManual Assertion Based On ExperimentTAS:UniProtKB
Negative regulation of cell population proliferationBy SimilarityISS:UniProtKB
Positive regulation of DNA biosynthetic processIEA:Ensembl
Positive regulation of ERK1 and ERK2 cascadeIEA:Ensembl
Positive regulation of MAP kinase activityIEA:Ensembl
Positive regulation of protein kinase B signalingIEA:Ensembl
Positive regulation of protein tyrosine kinase activityBy SimilarityISS:ARUK-UCL
Reactive oxygen species biosynthetic process1 PublicationNAS:ARUK-UCL
Reactive oxygen species metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Superoxide anion generationBy SimilarityISS:UniProtKB
Superoxide metabolic processManual Assertion Based On ExperimentIDA:CACAO
Cellular Location
Endoplasmic reticulum membrane
Cell membrane
Cell junction, focal adhesion
May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus.
Isoform 4
Nucleus
Nucleus, nucleolus
Topology
Cytoplasmic: 1-16
Helical: 17-37
Extracellular: 38-62
Helical: 63-83
Cytoplasmic: 84-103
Helical: 104-124
Extracellular: 125-154
Helical: 155-175
Cytoplasmic: 176-188
Helical: 189-209
Extracellular: 210-424
Helical: 425-445
Cytoplasmic: 446-578
PTM
Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function.
More Infomation

Luengo, E., Trigo-Alonso, P., Fernández-Mendívil, C., Nuñez, Á., Del Campo, M., Porrero, C., ... & López, M. G. (2022). Implication of type 4 NADPH oxidase (NOX4) in tauopathy. Redox Biology, 49, 102210.

Li, G., Ye, C., Zhu, Y., Zhang, T., Gu, J., Pan, J., ... & Shen, J. (2022). Oxidative injury in ischemic stroke: a focus on NADPH oxidase 4. Oxidative Medicine and Cellular Longevity, 2022.

Gong, S., Wang, S., & Shao, M. (2022). NADPH oxidase 4: a potential therapeutic target of malignancy. Frontiers in Cell and Developmental Biology, 10, 884412.

Szanto, I. (2022). NADPH Oxidase 4 (NOX4) in Cancer: Linking Redox Signals to Oncogenic Metabolic Adaptation. International Journal of Molecular Sciences, 23(5), 2702.

Piera-Velazquez, S., & Jimenez, S. A. (2021). Oxidative stress induced by reactive oxygen species (ROS) and NADPH oxidase 4 (NOX4) in the pathogenesis of the fibrotic process in systemic sclerosis: a promising therapeutic target. Journal of Clinical Medicine, 10(20), 4791.

Herranz-Itúrbide, M., López-Luque, J., Gonzalez-Sanchez, E., Caballero-Díaz, D., Crosas-Molist, E., Martín-Mur, B., ... & Fabregat, I. (2021). NADPH oxidase 4 (Nox4) deletion accelerates liver regeneration in mice. Redox biology, 40, 101841.

Helfinger, V., Palfi, K., Weigert, A., & Schröder, K. (2019). The NADPH oxidase Nox4 controls macrophage polarization in an NFκB-dependent manner. Oxidative Medicine and Cellular Longevity, 2019.

Rajaram, R. D., Dissard, R., Jaquet, V., & de Seigneux, S. (2019). Potential benefits and harms of NADPH oxidase type 4 in the kidneys and cardiovascular system. Nephrology Dialysis Transplantation, 34(4), 567-576.

Canugovi, C., Stevenson, M. D., Vendrov, A. E., Hayami, T., Robidoux, J., Xiao, H., ... & Madamanchi, N. R. (2019). Increased mitochondrial NADPH oxidase 4 (NOX4) expression in aging is a causative factor in aortic stiffening. Redox biology, 26, 101288.

Gray, S. P., Shah, A. M., & Smyrnias, I. (2019). NADPH oxidase 4 and its role in the cardiovascular system. Vascular Biology, 1(1), H59-H66.

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For research use only. Not intended for any clinical use.

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