Rabbit Anti-NOX4 Recombinant Antibody (A1390) (CBMAB-AP12724LY)

Name: Rabbit Anti-NOX4 Recombinant Antibody (A1390)
SKU: CBMAB-AP12724LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

A1390

Application

ICC, IF, IHC, WB

Immunogen

Recombinant protein of human NOX4

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

Affinity purity

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

NADPH Oxidase 4

Introduction

This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009]

Entrez Gene ID

Human	50507
Mouse	50490
Rat	85431

UniProt ID

Human	Q9NPH5
Mouse	Q9JHI8
Rat	Q924V1

Alternative Names

NADPH Oxidase 4; Kidney Superoxide-Producing NADPH Oxidase; Renal NAD(P)H-Oxidase; Kidney Oxidase-1; KOX-1; RENOX; EC 1.6.3.-; EC 1.6.3; KOX;

Function

A11904

Biological Process

Bone resorptionIEA:Ensembl
Cardiac muscle cell differentiationIEA:Ensembl
Cell agingBy SimilarityISS:UniProtKB
Cell morphogenesisBy SimilarityISS:UniProtKB
Cellular response to glucose stimulusIEA:Ensembl
Defense responseManual Assertion Based On ExperimentIBA:GO_Central
Gene expressionManual Assertion Based On ExperimentIMP:CACAO
Heart processManual Assertion Based On ExperimentIGI:ARUK-UCL
Homocysteine metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Inflammatory responseManual Assertion Based On ExperimentTAS:UniProtKB
Negative regulation of cell population proliferationBy SimilarityISS:UniProtKB
Positive regulation of DNA biosynthetic processIEA:Ensembl
Positive regulation of ERK1 and ERK2 cascadeIEA:Ensembl
Positive regulation of MAP kinase activityIEA:Ensembl
Positive regulation of protein kinase B signalingIEA:Ensembl
Positive regulation of protein tyrosine kinase activityBy SimilarityISS:ARUK-UCL
Reactive oxygen species biosynthetic process1 PublicationNAS:ARUK-UCL
Reactive oxygen species metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Superoxide anion generationBy SimilarityISS:UniProtKB
Superoxide metabolic processManual Assertion Based On ExperimentIDA:CACAO

Cellular Location

Endoplasmic reticulum membrane
Cell membrane
Cell junction, focal adhesion
May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus.
Isoform 4
Nucleus
Nucleus, nucleolus

Topology

Cytoplasmic: 1-16
Helical: 17-37
Extracellular: 38-62
Helical: 63-83
Cytoplasmic: 84-103
Helical: 104-124
Extracellular: 125-154
Helical: 155-175
Cytoplasmic: 176-188
Helical: 189-209
Extracellular: 210-424
Helical: 425-445
Cytoplasmic: 446-578

PTM

Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function.

Luengo, E., Trigo-Alonso, P., Fernández-Mendívil, C., Nuñez, Á., Del Campo, M., Porrero, C., ... & López, M. G. (2022). Implication of type 4 NADPH oxidase (NOX4) in tauopathy. Redox Biology, 49, 102210.

Li, G., Ye, C., Zhu, Y., Zhang, T., Gu, J., Pan, J., ... & Shen, J. (2022). Oxidative injury in ischemic stroke: a focus on NADPH oxidase 4. Oxidative Medicine and Cellular Longevity, 2022.

Gong, S., Wang, S., & Shao, M. (2022). NADPH oxidase 4: a potential therapeutic target of malignancy. Frontiers in Cell and Developmental Biology, 10, 884412.

Szanto, I. (2022). NADPH Oxidase 4 (NOX4) in Cancer: Linking Redox Signals to Oncogenic Metabolic Adaptation. International Journal of Molecular Sciences, 23(5), 2702.

Piera-Velazquez, S., & Jimenez, S. A. (2021). Oxidative stress induced by reactive oxygen species (ROS) and NADPH oxidase 4 (NOX4) in the pathogenesis of the fibrotic process in systemic sclerosis: a promising therapeutic target. Journal of Clinical Medicine, 10(20), 4791.

Herranz-Itúrbide, M., López-Luque, J., Gonzalez-Sanchez, E., Caballero-Díaz, D., Crosas-Molist, E., Martín-Mur, B., ... & Fabregat, I. (2021). NADPH oxidase 4 (Nox4) deletion accelerates liver regeneration in mice. Redox biology, 40, 101841.

Helfinger, V., Palfi, K., Weigert, A., & Schröder, K. (2019). The NADPH oxidase Nox4 controls macrophage polarization in an NFκB-dependent manner. Oxidative Medicine and Cellular Longevity, 2019.

Rajaram, R. D., Dissard, R., Jaquet, V., & de Seigneux, S. (2019). Potential benefits and harms of NADPH oxidase type 4 in the kidneys and cardiovascular system. Nephrology Dialysis Transplantation, 34(4), 567-576.

Canugovi, C., Stevenson, M. D., Vendrov, A. E., Hayami, T., Robidoux, J., Xiao, H., ... & Madamanchi, N. R. (2019). Increased mitochondrial NADPH oxidase 4 (NOX4) expression in aging is a causative factor in aortic stiffening. Redox biology, 26, 101288.

Gray, S. P., Shah, A. M., & Smyrnias, I. (2019). NADPH oxidase 4 and its role in the cardiovascular system. Vascular Biology, 1(1), H59-H66.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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