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Mouse Anti-NR4A1 Recombinant Antibody (12.14) (CBMAB-N0515-WJ)

This product is a Mouse antibody that recognizes NR4A1. The antibody 12.14 can be used for immunoassay techniques such as: WB.
See all NR4A1 antibodies

Summary

Host Animal
Mouse
Specificity
Mouse
Clone
12.14
Antibody Isotype
IgG1
Application
WB

Basic Information

Specificity
Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Preservative
0.09% sodium azide
Concentration
0.5 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Nuclear Receptor Subfamily 4 Group A Member 1
Introduction
This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
Entrez Gene ID
15370
UniProt ID
P12813
Alternative Names
Nuclear Receptor Subfamily 4 Group A Member 1; Nuclear Hormone Receptor NUR/77; Early Response Protein NAK1; Orphan Nuclear Receptor HMR; Orphan Nuclear Receptor TR3; Testicular Receptor 3; NUR77; GFRP1; ST-59; HMR; Nerve Growth Factor IB Nuclear Receptor Variant 1; Nuclear Receptor Subfamily 4, Group A, Member 1;
Function
Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (By similarity).
May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation. Plays a role in the vascular response to injury (By similarity).
Biological Process
Apoptotic processIEA:Ensembl
Cell migration involved in sprouting angiogenesisManual Assertion Based On ExperimentIDA:BHF-UCL
Cellular response to corticotropin-releasing hormone stimulusISS:UniProtKB
Cellular response to fibroblast growth factor stimulusManual Assertion Based On ExperimentIMP:BHF-UCL
Cellular response to vascular endothelial growth factor stimulusManual Assertion Based On ExperimentIMP:BHF-UCL
Endothelial cell chemotaxisManual Assertion Based On ExperimentIMP:BHF-UCL
Fat cell differentiationISS:UniProtKB
Negative regulation of cell cycleISS:UniProtKB
Negative regulation of cysteine-type endopeptidase activity involved in apoptotic processIEA:Ensembl
Neurotransmitter secretion involved in regulation of skeletal muscle contractionIEA:Ensembl
Positive regulation of apoptotic processIEA:Ensembl
Positive regulation of endothelial cell proliferationManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:MGI
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Regulation of type B pancreatic cell proliferationISS:UniProtKB
Response to amphetamineIEA:Ensembl
Response to electrical stimulusIEA:Ensembl
Response to lipopolysaccharideIEA:Ensembl
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Skeletal muscle cell differentiationIEA:Ensembl
Cellular Location
Nucleus
Cytoplasm
Mitochondrion
Nuclear export to the cytoplasm is XPO1-mediated and positively regulated by IFI27 (PubMed:22427340).
Translocation to the mitochondrion upon interaction with RXRA and upon the presence of 9-cis retinoic acid (PubMed:17761950).
PTM
Phosphorylated at Ser-351 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli.
Acetylated by p300/CBP, acetylation increases stability. Deacetylated by HDAC1.

Deng, S., Chen, B., Huo, J., & Liu, X. (2022). Therapeutic potential of NR4A1 in cancer: Focus on metabolism. Frontiers in oncology, 12, 972984.

Karki, K., Mohankumar, K., Schoeller, A., Martin, G., Shrestha, R., & Safe, S. (2021). NR4A1 ligands as potent inhibitors of breast cancer cell and tumor growth. Cancers, 13(11), 2682.

Safe, S., Shrestha, R., & Mohankumar, K. (2021). Orphan nuclear receptor 4A1 (NR4A1) and novel ligands. Essays in biochemistry, 65(6), 877-886.

Guo, H., Golczer, G., Wittner, B. S., Langenbucher, A., Zachariah, M., Dubash, T. D., ... & Haber, D. A. (2021). NR4A1 regulates expression of immediate early genes, suppressing replication stress in cancer. Molecular cell, 81(19), 4041-4058.

Li, W., Hang, S., Fang, Y., Bae, S., Zhang, Y., Zhang, M., ... & Huh, J. R. (2021). A bacterial bile acid metabolite modulates Treg activity through the nuclear hormone receptor NR4A1. Cell host & microbe, 29(9), 1366-1377.

Pulakazhi Venu, V. K., Alston, L., Iftinca, M., Tsai, Y. C., Stephens, M., Warriyar KV, V., ... & Hirota, S. A. (2021). Nr4A1 modulates inflammation-associated intestinal fibrosis and dampens fibrogenic signaling in myofibroblasts. American Journal of Physiology-Gastrointestinal and Liver Physiology, 321(3), G280-G297.

Jennings, E., Elliot, T. A., Thawait, N., Kanabar, S., Yam-Puc, J. C., Ono, M., ... & Bending, D. (2020). Nr4a1 and Nr4a3 reporter mice are differentially sensitive to T cell receptor signal strength and duration. Cell reports, 33(5).

Zárraga-Granados, G., Muciño-Hernández, G., Sánchez-Carbente, M. R., Villamizar-Gálvez, W., Peñas-Rincón, A., Arredondo, C., ... & Castro-Obregón, S. (2020). The nuclear receptor NR4A1 is regulated by SUMO modification to induce autophagic cell death. PloS one, 15(3), e0222072.

He, L., Yuan, L., Yu, W., Sun, Y., Jiang, D., Wang, X., ... & Zhang, L. (2020). A regulation loop between YAP and NR4A1 balances cell proliferation and apoptosis. Cell reports, 33(3).

Liu, X., Wang, Y., Lu, H., Li, J., Yan, X., Xiao, M., ... & Dong, C. (2019). Genome-wide analysis identifies NR4A1 as a key mediator of T cell dysfunction. Nature, 567(7749), 525-529.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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