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Mouse Anti-OTUD7B Recombinant Antibody (2B4) (CBMAB-A9996-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
2B4
Antibody Isotype
IgG2b, κ
Application
WB, ELISA

Basic Information

Immunogen
ZA20D1 (NP_064590, 759 a.a. ~ 858 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
OTU Deubiquitinase 7B
Entrez Gene ID
56957
UniProt ID
Q6GQQ9
Alternative Names
CEZANNE; ZA20D1
Function
Negative regulator of the non-canonical NF-kappa-B pathway that acts by mediating deubiquitination of TRAF3, an inhibitor of the NF-kappa-B pathway, thereby acting as a negative regulator of B-cell responses. In response to non-canonical NF-kappa-B stimuli, deubiquitinates 'Lys-48'-linked polyubiquitin chains of TRAF3, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B. Negatively regulates mucosal immunity against infections (By similarity).
Deubiquitinates ZAP70, and thereby regulates T cell receptor (TCR) signaling that leads to the activation of NF-kappa-B (PubMed:26903241).
Plays a role in T cell homeostasis and is required for normal T cell responses, including production of IFNG and IL2 (By similarity).
Mediates deubiquitination of EGFR (PubMed:22179831).
Has deubiquitinating activity toward 'Lys-11', 'Lys-48' and 'Lys-63'-linked polyubiquitin chains (PubMed:27732584).
Has a much higher catalytic rate with 'Lys-11'-linked polyubiquitin chains (in vitro); however the physiological significance of these data are unsure (PubMed:27732584).
Hydrolyzes both linear and branched forms of polyubiquitin.
Biological Process
Adaptive immune responseIEA:UniProtKB-KW
In utero embryonic developmentIEA:Ensembl
Mucosal immune responseISS:UniProtKB
Negative regulation of I-kappaB kinase/NF-kappaB signalingManual Assertion Based On ExperimentIDA:HGNC-UCL
Negative regulation of interleukin-8 productionManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Negative regulation of protein localization to nucleusManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Protein deubiquitinationTAS:Reactome
Protein deubiquitination involved in ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIBA:GO_Central
Protein K11-linked deubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Protein K48-linked deubiquitinationManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Protein K63-linked deubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Cytoplasm
Nucleus
Shuttles be cytoplasm and the nucleus in a XPO1/CRM1-dependent manner.
PTM
Phosphorylated by EGFR.
More Infomation

Chen, J., Bolhuis, D. L., Laggner, C., Kong, D., Yu, L., Wang, X., ... & Liu, P. (2023). AtomNet-aided OTUD7B inhibitor discovery and validation. Cancers, 15(2), 517.

Lee, Y., Piao, H. L., & Kim, J. (2023). OTUD7B Activates Wnt Signaling Pathway through the Interaction with LEF1. Biomolecules, 13(6), 1001.

Xie, W., Tian, S., Yang, J., Cai, S., Jin, S., Zhou, T., ... & Cui, J. (2022). OTUD7B deubiquitinates SQSTM1/p62 and promotes IRF3 degradation to regulate antiviral immunity. Autophagy, 18(10), 2288-2302.

Qiu, S., Liu, Y., Gui, A., Xia, Z., Liu, W., Gu, J. J., ... & Zhang, Q. (2022). Deubiquitinase OTUD7B is a potential prognostic biomarker in diffuse large B-cell lymphoma. Journal of Cancer, 13(3), 998.

Tang, J., Wu, Z., Tian, Z., Chen, W., & Wu, G. (2021). OTUD7B stabilizes estrogen receptor α and promotes breast cancer cell proliferation. Cell death & disease, 12(6), 534.

Gong, Z., Li, A., Ding, J., Li, Q., Zhang, L., Li, Y., ... & You, H. (2021). OTUD7B deubiquitinates LSD1 to govern its binding partner specificity, homeostasis, and breast cancer metastasis. Advanced Science, 8(15), 2004504.

Zhang, B., Yang, C., Wang, R., Wu, J., Zhang, Y., Liu, D., ... & Qin, S. (2020). OTUD7B suppresses Smac mimetic-induced lung cancer cell invasion and migration via deubiquitinating TRAF3. Journal of Experimental & Clinical Cancer Research, 39, 1-17.

Luo, Q., Zhu, J., Zhang, Q., Xie, J., Yi, C., & Li, T. (2020). MicroRNA-486-5p promotes acute lung injury via inducing inflammation and apoptosis by targeting OTUD7B. Inflammation, 43, 975-984.

Lei, S., He, Z., Chen, T., Guo, X., Zeng, Z., Shen, Y., & Jiang, J. (2019). Long noncoding RNA 00976 promotes pancreatic cancer progression through OTUD7B by sponging miR-137 involving EGFR/MAPK pathway. Journal of Experimental & Clinical Cancer Research, 38, 1-15.

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For research use only. Not intended for any clinical use.

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