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Rabbit Anti-PIN1 Recombinant Antibody (BA0021) (CBMAB-0650CQ)

This product is a rabbit antibody that recognizes PIN1. The antibody BA0021 can be used for immunoassay techniques such as: WB, ICC, FC.
See all PIN1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
BA0021
Antibody Isotype
IgG
Application
WB, ICC, FC

Basic Information

Immunogen
Synthetic peptide from residues near the N terminal of human Pin1
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
peptidylprolyl cis/trans isomerase, NIMA-interacting 1
Introduction
Peptidyl-prolyl cis/trans isomerases (PPIases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds. This gene encodes one of the PPIases, which specifically binds to phosphorylated ser/thr-pro motifs to catalytically regulate the post-phosphorylation conformation of its substrates. It binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs. By inducing conformational changes in a subset of phosphorylated proteins, acts as a molecular switch in multiple cellular processes (PubMed:21497122, PubMed:22033920, Ref. 21). Displays a preference for acidic residues located N-terminally to the proline bond to be isomerized. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK (PubMed:16644721). Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation.
Entrez Gene ID
Human5300
Mouse23988
Rat298696
UniProt ID
HumanQ13526
MouseQ9QUR7
RatB0BNL2
Alternative Names
DOD; UBL5
Function
Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs (PubMed:21497122, PubMed:23623683, PubMed:29686383).
By inducing conformational changes in a subset of phosphorylated proteins, acts as a molecular switch in multiple cellular processes (PubMed:21497122, PubMed:22033920, PubMed:23623683).
Displays a preference for acidic residues located N-terminally to the proline bond to be isomerized. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK (PubMed:16644721).
Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation (PubMed:15664191).
Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner (PubMed:17828269).
Acts as a regulator of JNK cascade by binding to phosphorylated FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation: degradation of FBXW7 leads to subsequent stabilization of JUN (PubMed:22608923).
May facilitate the ubiquitination and proteasomal degradation of RBBP8/CtIP through CUL3/KLHL15 E3 ubiquitin-protein ligase complex, hence favors DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR) (PubMed:23623683, PubMed:27561354).
Upon IL33-induced lung inflammation, catalyzes cis-trans isomerization of phosphorylated IRAK3/IRAK-M, inducing IRAK3 stabilization, nuclear translocation and expression of pro-inflammatory genes in dendritic cells (PubMed:29686383).
Biological Process
Cell cycleIEA:UniProtKB-KW
Negative regulation of amyloid-beta formationManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of cell motilityManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of ERK1 and ERK2 cascadeManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of protein bindingManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Negative regulation of protein catabolic processManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Negative regulation of transforming growth factor beta receptor signaling pathwayManual Assertion Based On ExperimentIDA:BHF-UCL
Neuron differentiationISS:ParkinsonsUK-UCL
Positive regulation of canonical Wnt signaling pathwayManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Positive regulation of GTPase activityManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of protein bindingManual Assertion Based On ExperimentIDA:ARUK-UCL
Positive regulation of protein dephosphorylationManual Assertion Based On ExperimentTAS:ARUK-UCL
Positive regulation of protein phosphorylationManual Assertion Based On ExperimentIGI:MGI
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Positive regulation of ubiquitin-protein transferase activityManual Assertion Based On ExperimentIDA:BHF-UCL
Protein peptidyl-prolyl isomerizationManual Assertion Based On ExperimentIDA:UniProtKB
Protein stabilizationManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Regulation of cytokinesisManual Assertion Based On ExperimentIMP:MGI
Regulation of gene expressionManual Assertion Based On ExperimentIDA:ARUK-UCL
Regulation of mitotic nuclear divisionManual Assertion Based On ExperimentTAS:ProtInc
Regulation of pathway-restricted SMAD protein phosphorylationManual Assertion Based On ExperimentIDA:BHF-UCL
Regulation of protein localization to nucleusManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Regulation of protein phosphorylationManual Assertion Based On ExperimentIDA:ARUK-UCL
Regulation of protein stabilityManual Assertion Based On ExperimentIMP:UniProtKB
Response to hypoxiaManual Assertion Based On ExperimentIDA:UniProtKB
Synapse organizationISS:ParkinsonsUK-UCL
Cellular Location
Nucleus
Nucleus speckle
Cytoplasm
Colocalizes with NEK6 in the nucleus (PubMed:16476580).
Mainly localized in the nucleus but phosphorylation at Ser-71 by DAPK1 results in inhibition of its nuclear localization (PubMed:21497122).
PTM
Phosphorylation at Ser-71 by DAPK1 results in inhibition of its catalytic activity, nuclear localization, and its ability to induce centrosome amplification, chromosome instability and cell transformation (PubMed:21497122).
Ser-71 is dephosphorylated upon IL33-stimulation of dendritic cells (By similarity).
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For research use only. Not intended for any clinical use.

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